FastQCFastQC Report
Wed 25 May 2016
SRR2088093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848122
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80630.9506886980882469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78770.9287578909638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64060.7553158625763746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.3729416286807794No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG21790.25692058453854516No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21700.2558594164518784No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG21300.2511431138444705No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20150.2375837438481728No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20020.2360509455007652No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18840.22213785280891193No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA11380.13417880918075467No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11350.13382508648519906No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA10650.12557155692223526No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC9920.11696430466371582No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.11484196849038228No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8910.1050556405800109No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA8900.1049377330148257No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.10446610275408491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC251.2333533E-436.9999961
GGTATCA21850.028.9565241
CCGTGTA400.001930772323.1259
ATAGTAC603.7248043E-521.5833323
ACTATCC450.003824993720.5555553
TTTCACG450.003824993720.55555529
GTATTGG2750.020.1818181
GATAGCA656.899406E-519.9230772
TAGGACC851.2445198E-619.5882364
ACTACCC500.007033400718.4999988
ATGCGAA500.007033400718.49999822
TTGGACC4050.018.2716054
GTATCAA35150.018.02
ATTGGAC2800.017.8392873
CAATCTA752.0664615E-417.26666610
TCGAACC1107.801664E-716.81818231
CCAGTAC1251.6560989E-716.2799993
CCTAGAC700.00259174215.8571433
TTATGCG957.056189E-515.5789484
ACGGGCG855.3620915E-415.2352945
Produced by FastQC (version 0.11.5)