FastQCFastQC Report
Wed 25 May 2016
SRR2088092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5251168
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT649801.2374389850029555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT637481.2139775379496522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT503950.9596912534506609No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250990.4779698535640071No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG189210.36031983741521884No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG183030.34855102712387037No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA172670.3288220830108654No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA164230.3127494683087648No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG154260.29376321610734984No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA140270.26712152420185376No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA97660.18597767201506407No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA88920.1693337558425097No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA83250.15853615805093269No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC83070.15819337716866036No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT78660.14979524555298937No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA76870.14638648011261493No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69560.1324657676158904No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG68540.13052334261634746No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG65020.12382007202968941No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA62980.11993522203060349No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG62130.11831653453098434No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57070.1086805830626634No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55970.10658581100433276No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC55710.1060906830632728No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT53230.10136792424085461No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC52690.10033958159403773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166350.029.4598731
GTATTGG19000.022.0052621
ATTGGAC22600.019.2367273
GTATCAA278650.017.6136722
TTGGACC33450.016.9237674
GGACCCT34450.015.5732956
TGGACCC34950.015.2975665
ACGTTTA3150.015.26984126
TAATACT13100.015.2519074
GACCCTC35450.014.6643157
TATTGGA29700.014.5757572
CTAGTAC7000.014.5357143
TAGTACT8000.014.3374994
CAATGCG4150.014.2650619
GTACTAG5900.014.1101691
CTAATAC11700.013.9145293
TGCGACG4300.013.76744222
GGGTAAG5650.013.7522131
CTCTAAT10300.013.4708731
CGTTTAG3300.013.45454526
Produced by FastQC (version 0.11.5)