##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5251168
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569329909079276	31.0	31.0	34.0	30.0	34.0
2	31.771961399825717	31.0	31.0	34.0	30.0	34.0
3	31.83799204291312	31.0	31.0	34.0	30.0	34.0
4	35.53640580533702	37.0	35.0	37.0	33.0	37.0
5	35.43573258368424	37.0	35.0	37.0	33.0	37.0
6	35.470433434999606	37.0	35.0	37.0	33.0	37.0
7	35.32289692502697	37.0	35.0	37.0	32.0	37.0
8	35.36902399618523	37.0	35.0	37.0	33.0	37.0
9	36.90286389618462	39.0	37.0	39.0	33.0	39.0
10	36.750974259440945	39.0	35.0	39.0	32.0	39.0
11	36.80009266509851	39.0	37.0	39.0	32.0	39.0
12	36.67580850584098	39.0	35.0	39.0	32.0	39.0
13	36.7008107529601	39.0	35.0	39.0	32.0	39.0
14	37.674879760083854	39.0	37.0	41.0	32.0	41.0
15	37.68293530125107	39.0	37.0	41.0	32.0	41.0
16	37.56230918530887	39.0	36.0	41.0	32.0	41.0
17	37.59193802216954	39.0	36.0	41.0	32.0	41.0
18	37.576965543665715	39.0	36.0	41.0	32.0	41.0
19	37.618680644001486	39.0	37.0	41.0	32.0	41.0
20	37.53737797000591	39.0	36.0	41.0	32.0	41.0
21	37.45403270281964	39.0	36.0	41.0	32.0	41.0
22	37.34090396650802	39.0	36.0	41.0	31.0	41.0
23	37.124637414000084	39.0	36.0	41.0	31.0	41.0
24	36.97505164565293	39.0	36.0	40.0	31.0	41.0
25	36.8255091057837	39.0	35.0	40.0	30.0	41.0
26	36.982491133401176	39.0	36.0	40.0	31.0	41.0
27	36.90674455663959	39.0	36.0	41.0	31.0	41.0
28	36.821545035314045	39.0	35.0	41.0	30.0	41.0
29	36.73038207880609	39.0	35.0	40.0	30.0	41.0
30	36.488923987958486	39.0	35.0	40.0	30.0	41.0
31	36.27342183681802	38.0	35.0	40.0	30.0	41.0
32	36.11377354523793	38.0	35.0	40.0	30.0	41.0
33	35.92681113992163	38.0	35.0	40.0	29.0	41.0
34	35.78239317424238	38.0	35.0	40.0	28.0	41.0
35	35.571002870218585	38.0	34.0	40.0	27.0	41.0
36	35.4668492800078	38.0	34.0	40.0	27.0	41.0
37	35.27845919231683	38.0	34.0	40.0	26.0	41.0
38	35.163956475968774	38.0	34.0	40.0	25.0	41.0
39	35.04709999756245	38.0	34.0	40.0	24.0	41.0
40	34.84356946873534	38.0	33.0	40.0	24.0	41.0
41	34.67360899517974	38.0	33.0	40.0	23.0	41.0
42	34.4299125451709	38.0	33.0	40.0	22.0	41.0
43	34.090646499978675	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	6.0
14	14.0
15	28.0
16	72.0
17	165.0
18	413.0
19	972.0
20	1981.0
21	4094.0
22	7566.0
23	13356.0
24	21592.0
25	32928.0
26	48198.0
27	67530.0
28	90724.0
29	118252.0
30	148386.0
31	182680.0
32	225321.0
33	277260.0
34	340067.0
35	416132.0
36	534239.0
37	729401.0
38	961869.0
39	1027911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.25123553464677	22.093656116125022	14.632306564939457	23.022801784288752
2	18.79437108087191	23.64470152164242	35.342556170360574	22.21837122712509
3	19.324767366041232	24.706027306686817	33.48639769285614	22.48280763441581
4	13.568010012248704	16.58410090859786	36.40313926349338	33.44474981566006
5	12.008261780998057	38.96992059671296	35.26853454317211	13.753283079116873
6	31.81456011310246	37.79191600801955	13.172288527047696	17.2212353518303
7	25.254743325675356	33.96465319715538	21.419025253048467	19.361578224120805
8	26.770387083406966	35.05145902778201	18.78437330513897	19.39378058367205
9	24.119662520795373	14.678448680369776	22.41924082413665	38.7826479746982
10	16.23343606603331	28.087560710302927	33.54011907446115	22.138884149202614
11	32.63365407467443	22.558828816750864	23.89925441349429	20.90826269508041
12	21.0203330002011	27.60067855379984	30.842147880243026	20.536840565756037
13	31.46061980877397	21.40771348393348	26.749153712088436	20.382512995204113
14	20.229137593769615	23.337550807744105	28.125571301470455	28.307740297015826
15	22.024242987464884	29.867279051060642	26.264518674702465	21.843959286772012
16	21.47312750230044	28.80972385572124	27.04891559363555	22.668233048342767
17	21.455131505981146	27.104884094357672	27.0544762612813	24.38550813837988
18	22.95607377253975	25.65848207484506	30.129125558351973	21.256318594263217
19	22.591507260860823	24.553185119958073	31.36108004923857	21.494227569942534
20	23.599511575329526	24.133164278880432	31.38252289776294	20.884801248027106
21	22.29629674769499	25.25527654038111	32.05064092407632	20.39778578784758
22	20.716705312037245	25.522759888847585	31.48451925362129	22.27601554549388
23	22.054179184516663	25.675125991017616	32.55797567322165	19.712719151244066
24	21.912896330873437	25.622680516029956	29.814319404749572	22.650103748347032
25	21.718349136801564	26.158009037227526	31.071201683130305	21.052440142840602
26	21.544825836842392	26.852273627505348	30.811278557456166	20.79162197819609
27	20.238297460679224	26.984510874533058	30.340240495067	22.436951169720718
28	20.743004223060467	26.36887640997203	30.778352549375683	22.10976681759182
29	21.202806689864044	26.143555110025048	29.920372001048147	22.73326619906276
30	21.42639504201732	26.422350227606508	31.630029738145875	20.521224992230298
31	22.34820900797689	26.100383762241087	31.29507568601881	20.256331543763217
32	21.721091383859743	26.027580911522925	30.278387589199202	21.972940115418133
33	21.218022352360467	25.913111140226324	31.166323377960865	21.70254312945234
34	20.283201756256894	26.664048836373166	31.89928412117076	21.153465286199186
35	19.911741540167828	28.873995271147297	30.365130195796443	20.84913299288844
36	22.326899463128967	26.265927884996255	30.277359246552386	21.1298134053224
37	21.41317893466749	27.081175083333843	30.22291421641814	21.282731765580536
38	21.169785464871815	25.38865639034973	31.649587291817742	21.791970852960713
39	20.72754099659352	25.273291580082756	32.380586566645746	21.61858085667798
40	19.33480322853887	25.986847116679566	31.896979871906595	22.78136978287497
41	21.126652203852554	24.85873618973912	31.998995271147297	22.015616335261033
42	19.610703752003364	27.331309910480865	31.11825788091335	21.939728456602417
43	20.16536130628462	27.19322634507218	30.11897543556024	22.522436913082956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	633.5
2	867.0
3	1642.5
4	2418.0
5	2418.0
6	4579.0
7	6740.0
8	7804.5
9	8869.0
10	14358.5
11	19848.0
12	19848.0
13	39736.5
14	59625.0
15	94392.0
16	129159.0
17	121019.0
18	112879.0
19	112879.0
20	123561.5
21	134244.0
22	108085.0
23	81926.0
24	91916.0
25	101906.0
26	101906.0
27	110869.5
28	119833.0
29	127246.0
30	134659.0
31	148527.0
32	162395.0
33	162395.0
34	181961.5
35	201528.0
36	223309.5
37	245091.0
38	259758.5
39	274426.0
40	274426.0
41	281555.0
42	288684.0
43	304088.5
44	319493.0
45	341712.0
46	363931.0
47	363931.0
48	478834.0
49	593737.0
50	538814.0
51	483891.0
52	434727.0
53	385563.0
54	385563.0
55	335441.5
56	285320.0
57	238941.0
58	192562.0
59	173183.5
60	153805.0
61	153805.0
62	136584.0
63	119363.0
64	104283.0
65	89203.0
66	76020.5
67	62838.0
68	62838.0
69	53375.5
70	43913.0
71	36017.0
72	28121.0
73	23250.5
74	18380.0
75	18380.0
76	14629.0
77	10878.0
78	8749.5
79	6621.0
80	5172.0
81	3723.0
82	3723.0
83	2842.5
84	1962.0
85	1526.0
86	1090.0
87	846.5
88	603.0
89	603.0
90	506.5
91	410.0
92	284.0
93	158.0
94	114.5
95	71.0
96	71.0
97	44.0
98	17.0
99	17.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5251168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.71749103999486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17165490013875	24.361838002571787
2	13.407980017688036	9.309828522570292
3	5.924217791516025	6.170219342878073
4	3.114084302570174	4.324527754890747
5	1.8492201812389464	3.2100142536570404
6	1.2126745682960949	2.526061107554959
7	0.8149964169515307	1.9806241562199882
8	0.5761468996757487	1.60018998617709
9	0.43751030953505937	1.3670334226069925
>10	2.1301294747648125	13.350828237953374
>50	0.18994009476723217	4.608387239916468
>100	0.14689113256092365	10.12784814277675
>500	0.014996012810396234	3.591027398464
>1k	0.008129705907922427	5.543557490754896
>5k	8.788871251807856E-4	2.119358812954293
>10k+	5.49304453237991E-4	5.808656128053189
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	64980	1.2374389850029555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	63748	1.2139775379496522	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	50395	0.9596912534506609	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25099	0.4779698535640071	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18921	0.36031983741521884	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	18303	0.34855102712387037	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	17267	0.3288220830108654	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	16423	0.3127494683087648	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	15426	0.29376321610734984	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	14027	0.26712152420185376	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9766	0.18597767201506407	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8892	0.1693337558425097	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8325	0.15853615805093269	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8307	0.15819337716866036	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7866	0.14979524555298937	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7687	0.14638648011261493	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6956	0.1324657676158904	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	6854	0.13052334261634746	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	6502	0.12382007202968941	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	6298	0.11993522203060349	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	6213	0.11831653453098434	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5707	0.1086805830626634	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5597	0.10658581100433276	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	5571	0.1060906830632728	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5323	0.10136792424085461	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5269	0.10033958159403773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.904338234846038E-5
2	0.0	0.0	0.0	0.0	1.904338234846038E-5
3	0.0	0.0	0.0	0.0	1.904338234846038E-5
4	0.0	0.0	0.0	1.904338234846038E-5	1.904338234846038E-5
5	0.0	0.0	0.0	1.904338234846038E-5	1.904338234846038E-5
6	0.0	0.0	0.0	1.904338234846038E-5	1.904338234846038E-5
7	0.0	0.0	0.0	5.7130147045381144E-5	1.1426029409076229E-4
8	0.0	0.0	0.0	9.52169117423019E-5	1.5234705878768305E-4
9	0.0	0.0	0.0	2.856507352269057E-4	1.7139044113614343E-4
10	0.0	0.0	0.0	4.1895441166612835E-4	1.7139044113614343E-4
11	0.0	0.0	0.0	6.855617645445737E-4	1.7139044113614343E-4
12	0.0	0.0	0.0	0.0010664294115137814	2.6660735287844535E-4
13	1.904338234846038E-5	0.0	0.0	0.0011616463232560833	2.6660735287844535E-4
14	1.904338234846038E-5	0.0	0.0	0.0014853838231799097	2.856507352269057E-4
15	1.904338234846038E-5	0.0	0.0	0.001942424999542959	2.856507352269057E-4
16	1.904338234846038E-5	0.0	0.0	0.002647030146435993	3.046941175753661E-4
17	1.904338234846038E-5	0.0	0.0	0.003808676469692076	3.2373749992382645E-4
18	1.904338234846038E-5	0.0	0.0	0.004570411763630491	3.2373749992382645E-4
19	1.904338234846038E-5	0.0	0.0	0.005808231616280416	3.2373749992382645E-4
20	1.904338234846038E-5	0.0	0.0	0.008969433086124839	3.2373749992382645E-4
21	1.904338234846038E-5	0.0	0.0	0.015177575731722924	3.2373749992382645E-4
22	1.904338234846038E-5	0.0	0.0	0.02443265955307467	3.2373749992382645E-4
23	1.904338234846038E-5	0.0	0.0	0.030107587492915863	3.2373749992382645E-4
24	1.904338234846038E-5	0.0	0.0	0.0381629382263146	3.2373749992382645E-4
25	1.904338234846038E-5	0.0	0.0	0.04277143675464201	3.2373749992382645E-4
26	1.904338234846038E-5	0.0	0.0	0.051264785282055345	3.2373749992382645E-4
27	1.904338234846038E-5	0.0	0.0	0.10426251835782058	3.808676469692076E-4
28	1.904338234846038E-5	0.0	0.0	0.20776330142170274	4.3799779401458875E-4
29	1.904338234846038E-5	0.0	0.0	0.33051694403987836	4.5704117636304915E-4
30	3.808676469692076E-5	0.0	0.0	0.5427744836958178	4.5704117636304915E-4
31	3.808676469692076E-5	0.0	0.0	0.8106006130445645	4.5704117636304915E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16635	0.0	29.459873	1
GTATTGG	1900	0.0	22.005262	1
ATTGGAC	2260	0.0	19.236727	3
GTATCAA	27865	0.0	17.613672	2
TTGGACC	3345	0.0	16.923767	4
GGACCCT	3445	0.0	15.573295	6
TGGACCC	3495	0.0	15.297566	5
ACGTTTA	315	0.0	15.269841	26
TAATACT	1310	0.0	15.251907	4
GACCCTC	3545	0.0	14.664315	7
TATTGGA	2970	0.0	14.575757	2
CTAGTAC	700	0.0	14.535714	3
TAGTACT	800	0.0	14.337499	4
CAATGCG	415	0.0	14.26506	19
GTACTAG	590	0.0	14.110169	1
CTAATAC	1170	0.0	13.914529	3
TGCGACG	430	0.0	13.767442	22
GGGTAAG	565	0.0	13.752213	1
CTCTAAT	1030	0.0	13.470873	1
CGTTTAG	330	0.0	13.454545	26
>>END_MODULE