FastQCFastQC Report
Wed 25 May 2016
SRR2088091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642805
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT505471.387584567386945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT501181.3758079282311295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT390451.07183887141914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181030.49695221127674966No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG146260.4015037862306657No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA140140.38470354575663535No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG136490.37468379449352907No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG126300.3467108450768021No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA112430.30863579027699806No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA102930.2825569856195981No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA77400.21247362952450102No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC62210.17077499344598462No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60750.1667670929407421No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58380.16026111746305388No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58250.15990424960984737No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA54930.15079039366641914No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53920.1480178049607377No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51280.14077064240331283No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG47200.12957048208729263No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45580.12512335960887283No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG45300.12435472115581263No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT44060.12095075086368884No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43960.12067623713045304No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC42230.11592714954547388No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.112660436119968No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38210.10489169746939514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120400.030.3467621
GTATTGG9500.018.6947361
GTATCAA205200.017.8148142
ACGTTTA3250.016.50769226
ATTGGAC12200.016.2254093
TTAACGG3600.015.93055635
TTGGACC19600.015.3852054
TTTTTAC23900.015.2489551
TCACGTT3650.014.6986324
GGACCCT19150.014.5874676
GTATTAG6500.014.2307691
TGGACCC19950.014.0952385
TAAACGT3200.013.8754
TGCGACG1601.7865932E-713.87522
GATACTG14850.013.70370436
TAACGGC4200.013.65476336
TATACTG3650.013.1780825
TGATACT15500.013.12903335
CGCCGGA3250.013.09230714
CAATGCG1703.7376958E-713.05882419
Produced by FastQC (version 0.11.5)