##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3642805
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273131282075212	31.0	31.0	33.0	30.0	34.0
2	31.4716741631792	31.0	31.0	34.0	30.0	34.0
3	31.52256214647778	31.0	31.0	34.0	30.0	34.0
4	35.27861332132793	37.0	35.0	37.0	33.0	37.0
5	35.14982904657263	35.0	35.0	37.0	32.0	37.0
6	35.19691117147363	37.0	35.0	37.0	32.0	37.0
7	35.04079603492364	37.0	35.0	37.0	32.0	37.0
8	35.0798263975151	37.0	35.0	37.0	32.0	37.0
9	36.57510874175258	39.0	35.0	39.0	32.0	39.0
10	36.40233062159517	38.0	35.0	39.0	32.0	39.0
11	36.47239723235254	38.0	35.0	39.0	32.0	39.0
12	36.33457678904031	38.0	35.0	39.0	32.0	39.0
13	36.38221562779232	38.0	35.0	39.0	32.0	39.0
14	37.25372425919038	39.0	36.0	40.0	32.0	41.0
15	37.2710035261289	39.0	36.0	40.0	32.0	41.0
16	37.11753332939864	39.0	36.0	40.0	31.0	41.0
17	37.19713737078982	39.0	36.0	40.0	31.0	41.0
18	37.19626716225546	39.0	36.0	40.0	31.0	41.0
19	37.242003620836144	39.0	36.0	40.0	31.0	41.0
20	37.178821265480856	39.0	36.0	40.0	31.0	41.0
21	37.08101998322721	39.0	36.0	40.0	31.0	41.0
22	36.99892527873438	39.0	36.0	40.0	31.0	41.0
23	36.80109970201534	39.0	35.0	40.0	30.0	41.0
24	36.642788730113196	39.0	35.0	40.0	30.0	41.0
25	36.5041227845026	38.0	35.0	40.0	30.0	41.0
26	36.57270674658677	38.0	35.0	40.0	30.0	41.0
27	36.4786932048243	38.0	35.0	40.0	30.0	41.0
28	36.37623205194898	38.0	35.0	40.0	30.0	41.0
29	36.259931838240036	38.0	35.0	40.0	30.0	41.0
30	36.02683399193753	38.0	35.0	40.0	29.0	41.0
31	35.81233719619908	38.0	34.0	40.0	29.0	41.0
32	35.6615396651756	38.0	34.0	40.0	28.0	41.0
33	35.47490354273698	38.0	34.0	40.0	27.0	41.0
34	35.32832693487573	38.0	34.0	40.0	27.0	41.0
35	35.09972397644123	38.0	34.0	40.0	26.0	41.0
36	34.97339879570825	38.0	33.0	40.0	25.0	41.0
37	34.7917313718412	38.0	33.0	40.0	25.0	41.0
38	34.65290483569667	38.0	33.0	40.0	24.0	41.0
39	34.54195324756609	38.0	33.0	40.0	24.0	41.0
40	34.3256133666227	38.0	33.0	40.0	23.0	41.0
41	34.162723780163915	38.0	33.0	40.0	22.0	41.0
42	33.933408184077926	38.0	33.0	40.0	20.0	41.0
43	33.57359919073352	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	21.0
15	16.0
16	44.0
17	142.0
18	357.0
19	841.0
20	1778.0
21	3544.0
22	6445.0
23	11207.0
24	18024.0
25	27656.0
26	40388.0
27	55492.0
28	74012.0
29	96266.0
30	120090.0
31	147051.0
32	177523.0
33	215699.0
34	258400.0
35	307552.0
36	385113.0
37	502333.0
38	629545.0
39	563261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.775008544239945	22.56991521643349	15.36524738491355	22.289828854413013
2	19.052762912096586	24.110678446966006	35.03566619679066	21.80089244414675
3	19.689003391617174	24.831276996709953	33.7081452342357	21.771574377437165
4	14.044424557449547	17.02355739601763	35.914384656878426	33.0176333896544
5	11.905331193956306	39.33004923403806	34.97900656225079	13.785613009754845
6	31.614017220246488	37.812839281817176	13.055873152694147	17.517270345242196
7	24.704753617061577	34.48027001170801	21.35041540790682	19.464560963323592
8	26.714880428680647	35.25006142244781	18.76095481366694	19.2741033352046
9	23.98593940658366	14.703779093308592	22.627755260026273	38.68252624008148
10	16.312649181056905	28.31894652609733	33.378207178259615	21.99019711458615
11	32.46034305981242	22.638735809355705	23.939985807639992	20.960935323191883
12	20.814811662990472	27.963149276450427	31.063232865882195	20.158806194676902
13	31.704524398094325	21.550563370809034	27.072983593686732	19.67192863740991
14	20.020368919006096	23.6602288620994	28.50048245788616	27.818919761008342
15	21.87237582028135	29.991915570556205	26.74628479976282	21.389423809399624
16	21.027916674101412	28.976050049343844	27.569386777497012	22.426646499057732
17	21.07384282167176	27.109960593553595	27.521868450273896	24.29432813450075
18	22.706870117944824	25.506580780469996	30.784793586261138	21.001755515324042
19	22.299107418596385	24.571806616055486	32.170291849275486	20.958794116072642
20	23.29951781662757	23.965872452684124	32.20990418098141	20.524705549706887
21	21.971667437592735	25.054209599470738	33.04714361597725	19.926979346959282
22	20.17052793108607	25.293063998759198	32.51236341226061	22.024044657894123
23	21.736134654476427	25.509874945268823	33.55554304992993	19.19844735032482
24	21.47899764055446	25.53801260292549	30.7342281565991	22.24876159992094
25	21.25104143647546	26.13120932907471	31.860612906812197	20.75713632763763
26	20.945562554130674	26.997080546447037	31.73878371200215	20.31857318742013
27	19.63695558779567	27.061646176504095	31.319326727617863	21.98207150808237
28	20.32153793573908	26.363091079538982	31.566883212249902	21.748487772472036
29	20.666107573696642	25.99944273712153	30.72791434073468	22.606535348447142
30	21.03900702892414	26.305800063412672	32.54162657622354	20.113566331439646
31	21.980040106456425	25.967901109172743	32.19609614019966	19.855962644171182
32	21.296693070312575	26.008584044438283	31.2255253849712	21.469197500277946
33	20.811874366044847	25.881401831830143	32.024607411047256	21.282116391077754
34	19.68219545103293	26.574576459623835	32.90845927794653	20.83476881139671
35	19.399995333266535	28.783368859985643	31.184979706572268	20.63165610017555
36	21.993216765651745	26.157095974118842	31.025844095415483	20.823843164813926
37	21.050783668079955	27.090964243213676	31.03095005085367	20.8273020378527
38	20.80605467490025	25.25853017111814	32.508959441968486	21.42645571201313
39	20.26874894483784	25.131183250270052	33.24589155883996	21.354176246052152
40	18.823818458577936	25.780710194479255	32.746962848683914	22.648508498258895
41	20.65770745345963	24.814559110355894	32.840324969357404	21.687408466827073
42	19.2852760441473	27.303053553511646	31.82942814671661	21.582242255624443
43	19.811271808400395	27.327567629889604	30.66831740924919	22.19284315246081
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	275.0
1	409.0
2	543.0
3	1153.5
4	1764.0
5	1764.0
6	3271.0
7	4778.0
8	5729.5
9	6681.0
10	10884.0
11	15087.0
12	15087.0
13	29904.0
14	44721.0
15	73138.5
16	101556.0
17	95313.0
18	89070.0
19	89070.0
20	96969.0
21	104868.0
22	83597.5
23	62327.0
24	67722.5
25	73118.0
26	73118.0
27	78996.0
28	84874.0
29	89829.5
30	94785.0
31	104203.0
32	113621.0
33	113621.0
34	126235.5
35	138850.0
36	153050.5
37	167251.0
38	175935.5
39	184620.0
40	184620.0
41	192396.5
42	200173.0
43	208777.0
44	217381.0
45	234861.0
46	252341.0
47	252341.0
48	341194.0
49	430047.0
50	385876.0
51	341705.0
52	302189.0
53	262673.0
54	262673.0
55	225643.0
56	188613.0
57	157373.0
58	126133.0
59	112157.0
60	98181.0
61	98181.0
62	86405.0
63	74629.0
64	64983.5
65	55338.0
66	46512.5
67	37687.0
68	37687.0
69	31947.0
70	26207.0
71	21696.5
72	17186.0
73	14038.5
74	10891.0
75	10891.0
76	8712.5
77	6534.0
78	5127.0
79	3720.0
80	2919.5
81	2119.0
82	2119.0
83	1643.0
84	1167.0
85	884.0
86	601.0
87	485.0
88	369.0
89	369.0
90	277.5
91	186.0
92	136.0
93	86.0
94	61.0
95	36.0
96	36.0
97	21.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3642805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.599633453243506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75079914108635	27.695545468232314
2	13.739521109316815	10.606809572854612
3	5.649181338888598	6.541689869760098
4	2.8850911861165693	4.454538490531324
5	1.6303320403090744	3.146510958150444
6	0.9604126410644598	2.22429455453698
7	0.6505215023774694	1.7576924081656515
8	0.4329721707882932	1.337005367030661
9	0.32157133409665317	1.1171282062681185
>10	1.6804260655619332	11.89130139749244
>50	0.16073633602814227	4.301533594435555
>100	0.12036912201167467	9.15136976936818
>500	0.010282631537979425	2.7033971238264174
>1k	0.006498051874084953	5.298995732254392
>5k	5.71257307611864E-4	1.3150812447130134
>10k+	7.140716345148299E-4	6.457106242379868
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50547	1.387584567386945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	50118	1.3758079282311295	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39045	1.07183887141914	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18103	0.49695221127674966	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	14626	0.4015037862306657	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	14014	0.38470354575663535	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13649	0.37468379449352907	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	12630	0.3467108450768021	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11243	0.30863579027699806	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10293	0.2825569856195981	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7740	0.21247362952450102	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6221	0.17077499344598462	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6075	0.1667670929407421	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5838	0.16026111746305388	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5825	0.15990424960984737	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5493	0.15079039366641914	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5392	0.1480178049607377	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5128	0.14077064240331283	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4720	0.12957048208729263	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4558	0.12512335960887283	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4530	0.12435472115581263	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4406	0.12095075086368884	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4396	0.12067623713045304	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4223	0.11592714954547388	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4104	0.112660436119968	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3821	0.10489169746939514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.7451373323578945E-5	0.0
5	0.0	0.0	0.0	2.7451373323578945E-5	0.0
6	2.7451373323578945E-5	0.0	0.0	2.7451373323578945E-5	0.0
7	5.490274664715789E-5	0.0	0.0	2.7451373323578945E-5	0.0
8	8.235411997073684E-5	0.0	0.0	2.7451373323578945E-5	0.0
9	8.235411997073684E-5	0.0	0.0	1.3725686661789473E-4	0.0
10	8.235411997073684E-5	0.0	0.0	4.392219731772631E-4	0.0
11	8.235411997073684E-5	0.0	0.0	6.862843330894737E-4	0.0
12	8.235411997073684E-5	0.0	0.0	9.607980663252631E-4	0.0
13	1.0980549329431578E-4	0.0	0.0	0.0011255063062667367	0.0
14	1.0980549329431578E-4	0.0	0.0	0.0012353117995610526	0.0
15	1.0980549329431578E-4	0.0	0.0	0.0017019851460618946	0.0
16	1.0980549329431578E-4	0.0	0.0	0.002552977719092842	0.0
17	1.0980549329431578E-4	0.0	0.0	0.0037882895186538944	0.0
18	1.0980549329431578E-4	0.0	0.0	0.00441967110509621	0.0
19	1.0980549329431578E-4	0.0	0.0	0.005682434277980842	0.0
20	1.0980549329431578E-4	0.0	0.0	0.01015700812972421	0.0
21	1.0980549329431578E-4	0.0	0.0	0.01737671931382547	0.0
22	1.0980549329431578E-4	0.0	0.0	0.028714136496463576	0.0
23	1.0980549329431578E-4	0.0	0.0	0.034478924894415154	0.0
24	1.0980549329431578E-4	0.0	0.0	0.042522177278223786	0.0
25	1.0980549329431578E-4	0.0	0.0	0.04765558408973305	0.0
26	1.0980549329431578E-4	0.0	0.0	0.05608315570007179	0.0
27	1.0980549329431578E-4	0.0	0.0	0.11392319929285262	0.0
28	1.0980549329431578E-4	0.0	0.0	0.23514846388977725	0.0
29	1.0980549329431578E-4	0.0	0.0	0.37800541066568205	0.0
30	1.0980549329431578E-4	0.0	0.0	0.6130989718088121	0.0
31	1.0980549329431578E-4	0.0	0.0	0.9203347420463077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12040	0.0	30.346762	1
GTATTGG	950	0.0	18.694736	1
GTATCAA	20520	0.0	17.814814	2
ACGTTTA	325	0.0	16.507692	26
ATTGGAC	1220	0.0	16.225409	3
TTAACGG	360	0.0	15.930556	35
TTGGACC	1960	0.0	15.385205	4
TTTTTAC	2390	0.0	15.248955	1
TCACGTT	365	0.0	14.69863	24
GGACCCT	1915	0.0	14.587467	6
GTATTAG	650	0.0	14.230769	1
TGGACCC	1995	0.0	14.095238	5
TAAACGT	320	0.0	13.875	4
TGCGACG	160	1.7865932E-7	13.875	22
GATACTG	1485	0.0	13.703704	36
TAACGGC	420	0.0	13.654763	36
TATACTG	365	0.0	13.178082	5
TGATACT	1550	0.0	13.129033	35
CGCCGGA	325	0.0	13.092307	14
CAATGCG	170	3.7376958E-7	13.058824	19
>>END_MODULE