FastQCFastQC Report
Wed 25 May 2016
SRR2088090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4172273
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT494641.185540831100937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT455861.0925938930650032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT390450.9358208343509641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178270.42727309550453674No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG139300.3338707702012788No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA128770.3086327284911606No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG127480.3055408886235393No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG119740.28698984941781136No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA113700.27251332786708826No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA101690.24372805902202468No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA75800.18167555191139217No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC61420.14720992610023362No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA61240.14677850658382136No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58580.14040308484128436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.135345889398896No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52430.12566291803053156No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA49660.11902385102796485No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.1132476230582227No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43970.10538620075915454No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG42110.10092819908956101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109750.029.2965831
GTATTGG12250.019.6326521
CGTTCGT701.2201734E-418.524
GTATTAG9350.016.8181821
GTATCAA192800.016.6864622
ATTGGAC15650.015.8402563
CGAATTA2300.015.28260715
GCCGTAT855.368353E-415.23529330
GGACCCT21200.015.1839636
TATACTG6650.014.744365
TAGGTCG2151.2732926E-1114.62790721
ATTAGAG8350.014.6227543
GTACTAG3550.014.591551
TTAACGG6600.014.57575935
GACCCTC21350.014.3840757
TGGACCC22600.014.3252215
TTGGACC23650.014.315014
TATACCG2600.014.2307695
TTATGCG3950.014.0506324
GACGCGA1458.925381E-714.03448333
Produced by FastQC (version 0.11.5)