##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4172273
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692971912432384	31.0	31.0	34.0	30.0	34.0
2	31.89159889585365	31.0	31.0	34.0	30.0	34.0
3	31.967201570942265	31.0	31.0	34.0	30.0	34.0
4	35.64504623738667	37.0	35.0	37.0	33.0	37.0
5	35.54876418681136	37.0	35.0	37.0	33.0	37.0
6	35.58817795479826	37.0	35.0	37.0	33.0	37.0
7	35.46185352684256	37.0	35.0	37.0	33.0	37.0
8	35.500035112755086	37.0	35.0	37.0	33.0	37.0
9	37.06824337717115	39.0	37.0	39.0	33.0	39.0
10	36.92056344347554	39.0	37.0	39.0	33.0	39.0
11	36.96502505948197	39.0	37.0	39.0	33.0	39.0
12	36.846464505079126	39.0	37.0	39.0	32.0	39.0
13	36.87412376898635	39.0	37.0	39.0	33.0	39.0
14	37.90159896056658	40.0	37.0	41.0	33.0	41.0
15	37.89236202904268	40.0	37.0	41.0	33.0	41.0
16	37.784277778563386	40.0	37.0	41.0	32.0	41.0
17	37.80076998796579	40.0	37.0	41.0	32.0	41.0
18	37.79138996896895	40.0	37.0	41.0	32.0	41.0
19	37.83687237148672	40.0	37.0	41.0	32.0	41.0
20	37.75622903870384	40.0	37.0	41.0	32.0	41.0
21	37.678119336869855	39.0	37.0	41.0	32.0	41.0
22	37.566303786928614	39.0	36.0	41.0	32.0	41.0
23	37.37906963422576	39.0	36.0	41.0	31.0	41.0
24	37.237120629450665	39.0	36.0	41.0	31.0	41.0
25	37.07139561577107	39.0	36.0	41.0	31.0	41.0
26	37.268131303967884	39.0	36.0	41.0	31.0	41.0
27	37.21441573933441	39.0	36.0	41.0	31.0	41.0
28	37.1483287407128	39.0	36.0	41.0	31.0	41.0
29	37.06344119859846	39.0	36.0	41.0	31.0	41.0
30	36.83596303501712	39.0	35.0	40.0	30.0	41.0
31	36.63369894539499	39.0	35.0	40.0	30.0	41.0
32	36.489948045106345	38.0	35.0	40.0	30.0	41.0
33	36.333701078524825	38.0	35.0	40.0	30.0	41.0
34	36.21378442877539	38.0	35.0	40.0	30.0	41.0
35	36.00508811384106	38.0	35.0	40.0	29.0	41.0
36	35.902399483447034	38.0	35.0	40.0	29.0	41.0
37	35.74128706343041	38.0	35.0	40.0	27.0	41.0
38	35.647648176425655	38.0	35.0	40.0	27.0	41.0
39	35.54875843455114	38.0	35.0	40.0	26.0	41.0
40	35.36328015928009	38.0	34.0	40.0	25.0	41.0
41	35.20016499399728	38.0	34.0	40.0	24.0	41.0
42	35.000264364292555	38.0	34.0	40.0	24.0	41.0
43	34.66130548025021	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	5.0
14	5.0
15	25.0
16	41.0
17	96.0
18	236.0
19	571.0
20	1214.0
21	2405.0
22	4650.0
23	8243.0
24	13723.0
25	21419.0
26	31222.0
27	44492.0
28	60796.0
29	81245.0
30	104111.0
31	130332.0
32	164043.0
33	205526.0
34	256215.0
35	320236.0
36	419017.0
37	583361.0
38	796230.0
39	922808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09399672552587	22.097475404893206	14.653954810723077	23.154573058857846
2	18.43167980618718	23.53944720299942	35.919821162229795	22.109051828583603
3	18.7424696322604	24.858512374429957	33.58541015892297	22.813607834386676
4	13.272693325676435	16.70358099769598	37.12305019350364	32.90067548312395
5	11.72360964874542	39.05405998121408	35.77805191558654	13.444278454453961
6	31.278945553179287	38.6870657792527	13.485670760278628	16.548317907289384
7	25.07575606869445	34.086288217477616	21.790903902980464	19.047051810847467
8	26.231960372679353	35.438836336931935	19.111069673532867	19.218133616855848
9	24.11687825796634	14.81348416079197	22.42029704192415	38.64934053931754
10	15.95372594266962	28.179148392255254	34.08597663671576	21.781149028359362
11	32.52795298869465	22.896608155794215	23.983138207878536	20.592300647632598
12	20.82183979811484	27.872049599822446	31.003316417693664	20.30279418436905
13	30.714409148202908	21.945376057606968	26.71244187520807	20.62777291898205
14	20.088905975232205	23.477131050628756	28.2101386941842	28.223824279954833
15	21.847132246619527	30.209911959260577	26.341972349364482	21.60098344475541
16	21.345774833046637	29.121632261359697	26.90487894727886	22.627713958314807
17	21.162733119333275	27.464645769823786	27.290759736958726	24.081861373884212
18	22.417852331331147	26.19277309993857	30.300557993208976	21.088816575521303
19	22.314767034659525	25.14672457914427	31.31935518121657	21.219153204979634
20	23.176455615440315	24.78373299158516	31.31748569664545	20.722325696329076
21	21.868751157941965	25.785297366687175	31.867401773565636	20.47854970180523
22	20.548271889207633	26.06833253720454	31.330404314386907	22.05299125920092
23	21.75936234278054	26.28684652226736	32.30440577594036	19.64938535901174
24	21.695799867362467	26.313738338790394	29.611581025498573	22.378880768348573
25	21.46674007189846	26.58387885931721	30.999889029313277	20.949492039471053
26	21.169875509104987	27.431905822078278	30.74166048098962	20.656558187827116
27	20.071577291322978	27.337760496496756	30.26542127037229	22.325240941807976
28	20.494272546403362	26.830818596961414	30.816727476845358	21.858181379789865
29	21.14931117882267	26.513701284647478	29.95098834616047	22.385999190369375
30	21.176922027873058	26.757669021178625	31.59821996307528	20.467188987873037
31	22.07892436568748	26.547280103674904	31.25411496323467	20.11968056740295
32	21.2225326578582	26.4257396388012	30.455773148113753	21.89595455522685
33	20.91330073559424	26.350552804190908	31.284985426409058	21.451161033805793
34	20.08346529577523	26.76629741150687	32.1587777214003	20.9914595713176
35	19.734135326235844	28.829009990477612	30.571992772285032	20.86486191100151
36	22.069121555564557	26.60027280094088	30.330877198112393	20.999728445382168
37	21.120334167970313	27.239636524263872	30.464257731936524	21.17577157582929
38	20.85469958461491	25.67161832411254	31.85951638351565	21.6141657077569
39	20.29397405203351	25.572775319352303	32.58653017192307	21.546720456691112
40	19.074422982388736	26.244543441908043	32.21788219514878	22.463151380554436
41	20.64469415112578	25.159595261383906	32.35452234309692	21.841188244393404
42	19.349644666108855	27.521281565228357	31.513661737858477	21.61541203080431
43	19.893640708553825	27.247953333830267	30.58076496911875	22.277640988497158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	466.0
2	658.0
3	1224.5
4	1791.0
5	1791.0
6	3319.0
7	4847.0
8	5676.0
9	6505.0
10	10562.0
11	14619.0
12	14619.0
13	28602.5
14	42586.0
15	69541.0
16	96496.0
17	91377.0
18	86258.0
19	86258.0
20	92870.0
21	99482.0
22	81695.5
23	63909.0
24	72495.0
25	81081.0
26	81081.0
27	90251.0
28	99421.0
29	108015.0
30	116609.0
31	129628.5
32	142648.0
33	142648.0
34	160599.0
35	178550.0
36	196153.0
37	213756.0
38	227777.0
39	241798.0
40	241798.0
41	248355.0
42	254912.0
43	266225.0
44	277538.0
45	287566.5
46	297595.0
47	297595.0
48	377692.0
49	457789.0
50	412590.0
51	367391.0
52	328870.0
53	290349.0
54	290349.0
55	253259.0
56	216169.0
57	179580.0
58	142991.0
59	127409.5
60	111828.0
61	111828.0
62	98808.0
63	85788.0
64	73807.5
65	61827.0
66	51681.0
67	41535.0
68	41535.0
69	35001.5
70	28468.0
71	23514.5
72	18561.0
73	15275.0
74	11989.0
75	11989.0
76	9477.0
77	6965.0
78	5680.0
79	4395.0
80	3349.5
81	2304.0
82	2304.0
83	1771.0
84	1238.0
85	950.5
86	663.0
87	498.5
88	334.0
89	334.0
90	274.5
91	215.0
92	151.0
93	87.0
94	58.5
95	30.0
96	30.0
97	21.5
98	13.0
99	12.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4172273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.91296738619468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.93923454134084	27.993690874950055
2	13.878939724217862	10.80141457686903
3	5.609249513293742	6.548176301154833
4	2.823527709244702	4.394873666554243
5	1.5788090790677989	3.071807310139666
6	0.9389589275793663	2.1922606875523325
7	0.6341362314404343	1.7273285744712215
8	0.4308257250690867	1.3411765910997635
9	0.32109867912621737	1.124541218572982
>10	1.5316434729498927	11.152734522078537
>50	0.1633397619835666	4.437037125305841
>100	0.1287509873440146	9.777497181346744
>500	0.013150697953311702	3.481536051754345
>1k	0.007347103535740082	5.671367514047001
>5k	3.704421950793319E-4	0.879171158340581
>10k+	6.174036584655532E-4	5.405386645762894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49464	1.185540831100937	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45586	1.0925938930650032	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39045	0.9358208343509641	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17827	0.42727309550453674	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	13930	0.3338707702012788	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12877	0.3086327284911606	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12748	0.3055408886235393	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11974	0.28698984941781136	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11370	0.27251332786708826	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10169	0.24372805902202468	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7580	0.18167555191139217	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6142	0.14720992610023362	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6124	0.14677850658382136	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5858	0.14040308484128436	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5647	0.135345889398896	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5243	0.12566291803053156	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4966	0.11902385102796485	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4725	0.1132476230582227	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4397	0.10538620075915454	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4211	0.10092819908956101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3967750911793164E-5	0.0
3	0.0	0.0	0.0	2.3967750911793164E-5	0.0
4	0.0	0.0	0.0	2.3967750911793164E-5	0.0
5	0.0	0.0	0.0	4.793550182358633E-5	0.0
6	0.0	0.0	0.0	4.793550182358633E-5	0.0
7	0.0	0.0	0.0	4.793550182358633E-5	0.0
8	0.0	0.0	0.0	4.793550182358633E-5	0.0
9	0.0	0.0	0.0	1.917420072943453E-4	0.0
10	0.0	0.0	0.0	2.636452600297248E-4	0.0
11	0.0	0.0	0.0	5.033227691476564E-4	0.0
12	0.0	0.0	0.0	9.347422855599334E-4	0.0
13	0.0	0.0	0.0	0.0010545810401188993	0.0
14	0.0	0.0	2.3967750911793164E-5	0.0012942585492368309	0.0
15	0.0	0.0	2.3967750911793164E-5	0.001725678065649108	0.0
16	0.0	0.0	2.3967750911793164E-5	0.0023967750911793166	0.0
17	0.0	0.0	2.3967750911793164E-5	0.0034273883803864224	0.0
18	0.0	0.0	4.793550182358633E-5	0.004194356409563804	0.0
19	0.0	0.0	4.793550182358633E-5	0.005033227691476565	0.0
20	0.0	0.0	4.793550182358633E-5	0.008963938841010644	0.0
21	0.0	0.0	4.793550182358633E-5	0.015938554356342453	0.0
22	0.0	0.0	4.793550182358633E-5	0.02562152572470689	0.0
23	0.0	0.0	7.190325273537949E-5	0.031014269679860353	0.0
24	0.0	0.0	7.190325273537949E-5	0.03765333668242706	0.0
25	0.0	0.0	7.190325273537949E-5	0.0417278543374319	0.0
26	0.0	0.0	7.190325273537949E-5	0.049733083141970814	0.0
27	0.0	0.0	7.190325273537949E-5	0.10121581210050253	0.0
28	0.0	0.0	7.190325273537949E-5	0.2110839822801624	0.0
29	0.0	0.0	7.190325273537949E-5	0.3457348069026164	0.0
30	0.0	0.0	7.190325273537949E-5	0.5644165662218172	0.0
31	0.0	0.0	7.190325273537949E-5	0.8733369077239194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10975	0.0	29.296583	1
GTATTGG	1225	0.0	19.632652	1
CGTTCGT	70	1.2201734E-4	18.5	24
GTATTAG	935	0.0	16.818182	1
GTATCAA	19280	0.0	16.686462	2
ATTGGAC	1565	0.0	15.840256	3
CGAATTA	230	0.0	15.282607	15
GCCGTAT	85	5.368353E-4	15.235293	30
GGACCCT	2120	0.0	15.183963	6
TATACTG	665	0.0	14.74436	5
TAGGTCG	215	1.2732926E-11	14.627907	21
ATTAGAG	835	0.0	14.622754	3
GTACTAG	355	0.0	14.59155	1
TTAACGG	660	0.0	14.575759	35
GACCCTC	2135	0.0	14.384075	7
TGGACCC	2260	0.0	14.325221	5
TTGGACC	2365	0.0	14.31501	4
TATACCG	260	0.0	14.230769	5
TTATGCG	395	0.0	14.050632	4
GACGCGA	145	8.925381E-7	14.034483	33
>>END_MODULE