FastQCFastQC Report
Wed 25 May 2016
SRR2088089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160700
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT500641.203259066983921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT470901.1317807099766866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT391210.9402504386281155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184830.44422813468887445No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG140710.338188285625015No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG136830.3288629317182205No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA127800.3071598529093662No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG119970.28834090417477826No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA116680.2804335808878314No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA112590.27060350421804025No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA72410.17403321556468862No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA63450.15249837767683322No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA62820.15098420938784338No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC62210.14951810993342468No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57900.13915927608335138No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56520.13584252649794507No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51360.12344076717859975No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47860.11502872112865623No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG45550.10947677073569351No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG44750.10755401735284928No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG44480.10690508808613935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.10591967697743168No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42180.10137717211046218No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC41680.10017545124618454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119800.028.8464091
TATGTCG502.703752E-422.25
GTATTGG14300.019.9230771
TGCGACG2300.017.69565222
TTGGACC25800.017.3527134
ATTGGAC17750.016.9887313
GTATCAA205800.016.7920322
TGGACCC26250.016.1390485
CAATGCG2300.016.08695619
TAATACT12850.015.9805444
GGACCCT26200.015.8167946
CTAATAC11000.015.6409093
CGTATTA957.066783E-515.57894815
GACCCTC26350.015.1650867
AATACTG12950.014.8571425
CGCGTAT908.280441E-414.38888824
CGAATTA2353.6379788E-1214.17021415
CGACGAG3150.014.09523924
ATACTGG11050.013.8959266
ACCGTCG2151.9826984E-1013.76744223
Produced by FastQC (version 0.11.5)