##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4160700
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59086187420386	31.0	31.0	34.0	30.0	34.0
2	31.785659143894055	31.0	31.0	34.0	30.0	34.0
3	31.858347153123272	31.0	31.0	34.0	30.0	34.0
4	35.55558151272622	37.0	35.0	37.0	33.0	37.0
5	35.44838801163266	37.0	35.0	37.0	33.0	37.0
6	35.48456990410268	37.0	35.0	37.0	33.0	37.0
7	35.346460932054704	37.0	35.0	37.0	33.0	37.0
8	35.38722835099863	37.0	35.0	37.0	33.0	37.0
9	36.93422404883793	39.0	37.0	39.0	33.0	39.0
10	36.78196817843151	39.0	37.0	39.0	32.0	39.0
11	36.822815631985	39.0	37.0	39.0	32.0	39.0
12	36.700578027735716	39.0	35.0	39.0	32.0	39.0
13	36.73710337202875	39.0	35.0	39.0	32.0	39.0
14	37.72478717523494	39.0	37.0	41.0	32.0	41.0
15	37.725865118850194	39.0	37.0	41.0	32.0	41.0
16	37.60657125003004	39.0	36.0	41.0	32.0	41.0
17	37.63204508856683	39.0	36.0	41.0	32.0	41.0
18	37.61893984185353	39.0	36.0	41.0	32.0	41.0
19	37.65888191890787	39.0	37.0	41.0	32.0	41.0
20	37.585974956137186	39.0	36.0	41.0	32.0	41.0
21	37.49818732424832	39.0	36.0	41.0	32.0	41.0
22	37.391055591607184	39.0	36.0	41.0	32.0	41.0
23	37.19723339822626	39.0	36.0	41.0	31.0	41.0
24	37.05208474535535	39.0	36.0	41.0	31.0	41.0
25	36.89827721296897	39.0	36.0	40.0	30.0	41.0
26	37.06915831470666	39.0	36.0	41.0	31.0	41.0
27	37.005021991491816	39.0	36.0	41.0	31.0	41.0
28	36.93323767635254	39.0	36.0	41.0	31.0	41.0
29	36.84345014060134	39.0	35.0	40.0	30.0	41.0
30	36.61634027927993	39.0	35.0	40.0	30.0	41.0
31	36.41064652582498	38.0	35.0	40.0	30.0	41.0
32	36.27142716369842	38.0	35.0	40.0	30.0	41.0
33	36.099441440142286	38.0	35.0	40.0	30.0	41.0
34	35.965994664359364	38.0	35.0	40.0	29.0	41.0
35	35.75672771408657	38.0	35.0	40.0	28.0	41.0
36	35.65265195760329	38.0	35.0	40.0	27.0	41.0
37	35.480475881462255	38.0	34.0	40.0	27.0	41.0
38	35.38912634893167	38.0	34.0	40.0	26.0	41.0
39	35.278960751796575	38.0	34.0	40.0	25.0	41.0
40	35.09530535727161	38.0	34.0	40.0	24.0	41.0
41	34.94143317230274	38.0	34.0	40.0	24.0	41.0
42	34.72751171677843	38.0	33.0	40.0	23.0	41.0
43	34.39768043838777	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	6.0
14	12.0
15	13.0
16	52.0
17	98.0
18	268.0
19	602.0
20	1374.0
21	2773.0
22	5300.0
23	9193.0
24	15465.0
25	24176.0
26	34757.0
27	50188.0
28	67666.0
29	89497.0
30	114113.0
31	141832.0
32	175456.0
33	216083.0
34	265898.0
35	326455.0
36	420292.0
37	574396.0
38	772640.0
39	852089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.216045376979835	22.05145768740837	14.61972744970798	23.112769485903815
2	18.51558631960968	23.561275746869516	35.71514408633163	22.207993847189176
3	19.06984401663182	24.748599995193114	33.397048573557335	22.78450741461773
4	13.449635878578123	16.549402744730454	36.75182060710938	33.24914076958204
5	11.852548849953132	39.01963611892229	35.62537553777009	13.502439493354485
6	31.6995457495133	38.255173408320715	13.35616602975461	16.689114812411372
7	25.279496238613696	33.882736078063786	21.603888768716804	19.233878914605715
8	26.45997067801091	35.47753983704665	18.97644627106016	19.086043213882277
9	24.15845891316365	14.741365635590164	22.233061744418006	38.86711370682818
10	15.997452351767732	28.227750138197898	33.89946403249453	21.875333477539836
11	32.78825678371428	22.70541014733098	23.776191506236934	20.73014156271781
12	20.806907491527866	27.709928617780662	31.03201384382436	20.451150046867113
13	31.095873290552074	21.632682000624897	26.754007739082365	20.517436969740668
14	20.212608455308	23.26437859014108	28.137741245463506	28.385271709087412
15	21.915014300478287	30.019323671497585	26.270074747037757	21.79558728098637
16	21.42187612661331	29.0833513591463	26.857259595741102	22.63751291849929
17	21.211743216285722	27.320547023337422	27.250270387194465	24.217439373182398
18	22.687816953877952	25.901362751460088	30.22385656259764	21.186963732064314
19	22.40339365972072	24.884250246352778	31.276660177373998	21.435695916552504
20	23.306943543153796	24.551974427380006	31.38200302833658	20.759079001129617
21	22.146537842190018	25.489100391761	31.85262095320499	20.511740812843993
22	20.597591751387988	25.841108467325206	31.388660561924674	22.172639219362125
23	21.91883577282669	25.944144014228378	32.370851058716084	19.766169154228855
24	21.788665368808132	25.98514673011753	29.738769918523328	22.487417982551015
25	21.573821712692578	26.36231884057971	30.946186939697647	21.117672507030065
26	21.442329415723314	27.095272430119934	30.707861657894103	20.75453649626265
27	20.225659143894056	27.190809238830006	30.344893888047686	22.238637729228255
28	20.75263777729709	26.45494748479823	30.80938303650828	21.9830317013964
29	21.23137933520802	26.253659240031723	29.961232484918405	22.553728939841854
30	21.34599947124282	26.371019299637084	31.639171293291994	20.643809935828106
31	22.16612589227774	26.28682673588579	31.31946547455957	20.2275818972769
32	21.465426490734732	26.138991035162352	30.3892854567741	22.006297017328816
33	21.156247746773378	26.059365010695316	31.28687480472036	21.497512437810943
34	20.181748263513352	26.73105487057466	31.97296128055375	21.11423558535823
35	19.900425409185953	28.728507222342394	30.435143124954934	20.935924243516716
36	22.263104766024945	26.303746965654817	30.32179681303627	21.111351455283966
37	21.26740692671906	27.046386425361117	30.433124233902948	21.253082414016873
38	21.02675030643882	25.505107313673182	31.672771408657198	21.795370971230803
39	20.52930997187973	25.395846852693055	32.47443939721681	21.6004037782104
40	19.30497272093638	26.119042468815344	32.0176412622876	22.55834354796068
41	20.913476097772012	24.985435143124953	32.0864998678107	22.01458889129233
42	19.544043069675777	27.338500733049724	31.28884562693777	21.828610570336725
43	20.09878145504362	27.08657197106256	30.365587521330546	22.44905905256327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	479.0
2	680.0
3	1321.5
4	1963.0
5	1963.0
6	3485.5
7	5008.0
8	5760.0
9	6512.0
10	10686.0
11	14860.0
12	14860.0
13	29220.0
14	43580.0
15	70682.0
16	97784.0
17	92338.5
18	86893.0
19	86893.0
20	94917.0
21	102941.0
22	83553.0
23	64165.0
24	72249.0
25	80333.0
26	80333.0
27	88989.5
28	97646.0
29	105925.5
30	114205.0
31	125511.0
32	136817.0
33	136817.0
34	153174.0
35	169531.0
36	185749.0
37	201967.0
38	213931.0
39	225895.0
40	225895.0
41	232735.0
42	239575.0
43	251355.5
44	263136.0
45	278692.0
46	294248.0
47	294248.0
48	376719.5
49	459191.0
50	416596.0
51	374001.0
52	337319.5
53	300638.0
54	300638.0
55	261224.0
56	221810.0
57	186863.5
58	151917.0
59	136218.5
60	120520.0
61	120520.0
62	106021.0
63	91522.0
64	79437.5
65	67353.0
66	56376.5
67	45400.0
68	45400.0
69	38226.0
70	31052.0
71	25496.5
72	19941.0
73	16300.5
74	12660.0
75	12660.0
76	10123.5
77	7587.0
78	5968.5
79	4350.0
80	3329.5
81	2309.0
82	2309.0
83	1758.0
84	1207.0
85	905.5
86	604.0
87	450.5
88	297.0
89	297.0
90	240.0
91	183.0
92	137.0
93	91.0
94	65.0
95	39.0
96	39.0
97	23.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4160700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.38066129072535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18366026950389	29.148239360676246
2	13.850256211269308	11.185650097140616
3	5.5963485786830764	6.779527692619006
4	2.767714219377573	4.4704852176884025
5	1.5650907892918133	3.159970052581336
6	0.90875933508594	2.2017781742934077
7	0.6174196784376565	1.7452270436453698
8	0.4176082133420991	1.3490636652153818
9	0.2951604705003763	1.0726897487118143
>10	1.524091267435863	11.344368766039336
>50	0.14802119626027474	4.18402026056775
>100	0.10752545134260709	8.620682258830538
>500	0.010839825159465287	2.9251434513079
>1k	0.00649198317290649	5.250178524583944
>5k	4.1691635055362777E-4	1.02616316736032
>10k+	5.955947865051826E-4	5.536812518738684
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50064	1.203259066983921	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	47090	1.1317807099766866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39121	0.9402504386281155	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18483	0.44422813468887445	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	14071	0.338188285625015	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13683	0.3288629317182205	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12780	0.3071598529093662	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11997	0.28834090417477826	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11668	0.2804335808878314	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11259	0.27060350421804025	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7241	0.17403321556468862	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6345	0.15249837767683322	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6282	0.15098420938784338	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6221	0.14951810993342468	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5790	0.13915927608335138	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5652	0.13584252649794507	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5136	0.12344076717859975	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4786	0.11502872112865623	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4555	0.10947677073569351	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4475	0.10755401735284928	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4448	0.10690508808613935	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4407	0.10591967697743168	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4218	0.10137717211046218	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4168	0.10017545124618454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.403441728555291E-5	0.0	0.0	0.0	0.0
3	2.403441728555291E-5	2.403441728555291E-5	0.0	2.403441728555291E-5	0.0
4	2.403441728555291E-5	2.403441728555291E-5	0.0	2.403441728555291E-5	0.0
5	2.403441728555291E-5	2.403441728555291E-5	0.0	2.403441728555291E-5	0.0
6	7.210325185665874E-5	2.403441728555291E-5	0.0	2.403441728555291E-5	0.0
7	7.210325185665874E-5	2.403441728555291E-5	0.0	2.403441728555291E-5	0.0
8	7.210325185665874E-5	2.403441728555291E-5	0.0	2.403441728555291E-5	0.0
9	7.210325185665874E-5	2.403441728555291E-5	0.0	7.210325185665874E-5	0.0
10	7.210325185665874E-5	4.806883457110582E-5	0.0	2.1630975556997622E-4	0.0
11	7.210325185665874E-5	4.806883457110582E-5	0.0	4.3261951113995244E-4	0.0
12	7.210325185665874E-5	4.806883457110582E-5	0.0	7.210325185665874E-4	0.0
13	7.210325185665874E-5	4.806883457110582E-5	0.0	8.17170187708799E-4	0.0
14	7.210325185665874E-5	4.806883457110582E-5	0.0	0.0011776864469920927	0.0
15	7.210325185665874E-5	9.613766914221164E-5	0.0	0.0016824092099887039	0.0
16	9.613766914221164E-5	9.613766914221164E-5	0.0	0.0024034417285552913	0.0
17	9.613766914221164E-5	9.613766914221164E-5	0.0	0.0037493690965462543	0.0
18	9.613766914221164E-5	9.613766914221164E-5	0.0	0.004662676953397265	0.0
19	9.613766914221164E-5	9.613766914221164E-5	0.0	0.006104741990530439	0.0
20	9.613766914221164E-5	9.613766914221164E-5	0.0	0.009589732496935613	0.0
21	9.613766914221164E-5	9.613766914221164E-5	0.0	0.017304780445598095	0.0
22	9.613766914221164E-5	9.613766914221164E-5	0.0	0.02824044031052467	0.0
23	9.613766914221164E-5	9.613766914221164E-5	0.0	0.03367221861705963	0.0
24	9.613766914221164E-5	9.613766914221164E-5	0.0	0.042036195832432045	0.0
25	9.613766914221164E-5	9.613766914221164E-5	0.0	0.04643449419568822	0.0
26	9.613766914221164E-5	9.613766914221164E-5	0.0	0.054582161655490666	0.0
27	9.613766914221164E-5	9.613766914221164E-5	0.0	0.10649650299228496	0.0
28	9.613766914221164E-5	9.613766914221164E-5	0.0	0.2133535222438532	0.0
29	9.613766914221164E-5	9.613766914221164E-5	0.0	0.3441728555291177	0.0
30	1.2017208642776456E-4	9.613766914221164E-5	0.0	0.5551469704617011	0.0
31	1.4420650371331747E-4	9.613766914221164E-5	0.0	0.8318071478357008	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11980	0.0	28.846409	1
TATGTCG	50	2.703752E-4	22.2	5
GTATTGG	1430	0.0	19.923077	1
TGCGACG	230	0.0	17.695652	22
TTGGACC	2580	0.0	17.352713	4
ATTGGAC	1775	0.0	16.988731	3
GTATCAA	20580	0.0	16.792032	2
TGGACCC	2625	0.0	16.139048	5
CAATGCG	230	0.0	16.086956	19
TAATACT	1285	0.0	15.980544	4
GGACCCT	2620	0.0	15.816794	6
CTAATAC	1100	0.0	15.640909	3
CGTATTA	95	7.066783E-5	15.578948	15
GACCCTC	2635	0.0	15.165086	7
AATACTG	1295	0.0	14.857142	5
CGCGTAT	90	8.280441E-4	14.388888	24
CGAATTA	235	3.6379788E-12	14.170214	15
CGACGAG	315	0.0	14.095239	24
ATACTGG	1105	0.0	13.895926	6
ACCGTCG	215	1.9826984E-10	13.767442	23
>>END_MODULE