FastQCFastQC Report
Wed 25 May 2016
SRR2088088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4665744
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT569431.2204484429492917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT541871.161379621342277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT440830.9448225191952239No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208260.44635968025678224No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG157400.33735241367721847No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG151130.32391404243353256No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA145620.31210456467392983No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG136460.2924721116289278No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA133450.2860208361195985No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA122980.2635806850954532No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA84910.18198598122828855No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA73560.15765974301204694No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72880.15620231199997256No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC71630.15352321087483584No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66670.14289253761029325No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA64330.13787726030403724No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA57180.1225528018682551No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.11916641804608226No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG55240.11839483692204288No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG52880.1133366939977847No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG52410.11232935197473329No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA48890.10478500320634822No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47310.10139861938417538No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC47020.10077706792314366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144950.028.7295631
GTATTGG17050.018.2287391
ATTGGAC17650.017.2946173
GTATCAA244550.017.021062
TTAACGG5400.016.78703735
GGACCCT27450.015.3661216
GTACTAG4950.015.3232311
TTGGACC28350.015.2698414
GTATTAG9600.015.2239591
TATACCG3050.015.1639345
TGCGACG2650.014.660377522
ATTAGAG9150.014.5573783
ACGTTTA2550.014.50980426
TGGACCC28550.014.4500885
AACGGCC6700.014.35820937
TACTCCG10800.014.2175925
TAACGGC6400.014.164062536
CTAATAC9200.014.0760873
TCCGGTC9100.014.02747258
CGAATTA3450.013.40579715
Produced by FastQC (version 0.11.5)