##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4665744
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522304267015077	31.0	31.0	34.0	30.0	34.0
2	31.71436602608287	31.0	31.0	34.0	30.0	34.0
3	31.779450822848403	31.0	31.0	34.0	30.0	34.0
4	35.496833516798176	37.0	35.0	37.0	33.0	37.0
5	35.38114178574735	37.0	35.0	37.0	33.0	37.0
6	35.420923436862374	37.0	35.0	37.0	33.0	37.0
7	35.28099891464255	37.0	35.0	37.0	32.0	37.0
8	35.30055570987178	37.0	35.0	37.0	32.0	37.0
9	36.847532354968465	39.0	37.0	39.0	33.0	39.0
10	36.690666268873734	39.0	35.0	39.0	32.0	39.0
11	36.75147564889973	39.0	35.0	39.0	32.0	39.0
12	36.62720286410913	39.0	35.0	39.0	32.0	39.0
13	36.66648684539915	39.0	35.0	39.0	32.0	39.0
14	37.62660874664362	39.0	36.0	41.0	32.0	41.0
15	37.63424439917835	39.0	36.0	41.0	32.0	41.0
16	37.5009141093039	39.0	36.0	41.0	32.0	41.0
17	37.53559518053284	39.0	36.0	41.0	32.0	41.0
18	37.5276678274676	39.0	36.0	41.0	32.0	41.0
19	37.56240891056174	39.0	36.0	41.0	32.0	41.0
20	37.4869930712015	39.0	36.0	41.0	32.0	41.0
21	37.40382798541883	39.0	36.0	41.0	32.0	41.0
22	37.31067521064165	39.0	36.0	41.0	31.0	41.0
23	37.12735846630248	39.0	36.0	40.0	31.0	41.0
24	36.980272813939216	39.0	36.0	40.0	31.0	41.0
25	36.83345228542329	39.0	35.0	40.0	30.0	41.0
26	36.95256169219743	39.0	36.0	40.0	31.0	41.0
27	36.87588153143422	39.0	35.0	40.0	31.0	41.0
28	36.78881588874143	39.0	35.0	40.0	30.0	41.0
29	36.67708472646592	39.0	35.0	40.0	30.0	41.0
30	36.45941569018789	38.0	35.0	40.0	30.0	41.0
31	36.2723188413252	38.0	35.0	40.0	30.0	41.0
32	36.125082301986566	38.0	35.0	40.0	30.0	41.0
33	35.96269212369989	38.0	35.0	40.0	29.0	41.0
34	35.82008549976167	38.0	35.0	40.0	29.0	41.0
35	35.606006458991324	38.0	34.0	40.0	27.0	41.0
36	35.490072322870695	38.0	34.0	40.0	27.0	41.0
37	35.32178233525028	38.0	34.0	40.0	26.0	41.0
38	35.20269950515931	38.0	34.0	40.0	25.0	41.0
39	35.1084185073163	38.0	34.0	40.0	25.0	41.0
40	34.909609056990696	38.0	33.0	40.0	24.0	41.0
41	34.7610541855704	38.0	33.0	40.0	24.0	41.0
42	34.54174489641952	38.0	33.0	40.0	23.0	41.0
43	34.19103234125147	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	9.0
15	26.0
16	50.0
17	135.0
18	355.0
19	779.0
20	1659.0
21	3286.0
22	6228.0
23	10914.0
24	18334.0
25	28368.0
26	41238.0
27	58482.0
28	79839.0
29	105009.0
30	133987.0
31	166117.0
32	204850.0
33	252674.0
34	309415.0
35	377538.0
36	480535.0
37	642131.0
38	840295.0
39	903484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.403759829086205	21.810047872322187	14.650482323933762	23.135709974657846
2	18.65906059140836	23.450536506074915	35.47905757366885	22.411345328847876
3	19.280654918058087	24.589433110775044	33.246444725642895	22.883467245523974
4	13.570032989379616	16.450795414407647	36.3107148613383	33.66845673487443
5	12.136628156195453	38.803114787266516	35.26481521489392	13.795441841644118
6	31.91782489566509	37.676499182124005	13.316311396424666	17.08936452578624
7	25.38628351662672	33.7342983241258	21.484226309887553	19.39519184935993
8	26.627050262509044	35.005221032272665	18.95899132056967	19.40873738464862
9	24.13062096848863	14.703378496548462	22.33485163352297	38.831148901439946
10	16.235095624620637	27.94383918191825	33.69529061174381	22.1257745817173
11	32.76772150379447	22.53824899094335	23.8068140901001	20.887215415162082
12	20.997572948708715	27.49242135873721	30.90201691305824	20.60798877949583
13	31.297109314184407	21.35325041408187	26.821102915204946	20.528537356528776
14	20.299013404936062	23.18830608794653	28.108164528529638	28.404515978587767
15	22.02883398660535	29.799448919614964	26.185877322030528	21.98583977174916
16	21.557097860491275	28.726158143267185	26.970339564279566	22.746404431961974
17	21.42063516558131	27.061171808826202	27.093771111316865	24.424421914275623
18	22.902885370478963	25.555795603016367	30.156198025438176	21.385121001066494
19	22.644127067408757	24.58476075841281	31.28671440181887	21.484397772359564
20	23.487550967219807	24.249080103837674	31.27394044765422	20.9894284812883
21	22.266995360225508	25.246798795647596	31.883253774746322	20.602952069380574
22	20.77405018363631	25.47786590948839	31.391692300306236	22.35639160656907
23	21.99490584995662	25.670139639037203	32.451416108556316	19.883538402449854
24	21.92267728362293	25.703081866471884	29.75392563329664	22.620315216608542
25	21.715293423728347	26.111226848279717	30.982411379621343	21.191068348370592
26	21.518990326087327	26.822667510262026	30.742814007798113	20.915528155852527
27	20.26941469570555	26.941555301791098	30.328282048907955	22.460747953595398
28	20.81175049466923	26.24280286273743	30.720159528683954	22.225287113909378
29	21.21217966523667	26.15098470897675	29.9021335075392	22.73470211824738
30	21.39639465860107	26.292891337372986	31.594510971883583	20.716203032142356
31	22.29648690541101	26.067889708479502	31.33937481353456	20.29624857257492
32	21.617281188166345	25.954402984818714	30.37048324983111	22.05783257718383
33	21.16457739644524	25.91119444187251	31.19648227592427	21.727745885757983
34	20.319203111015092	26.51787153345747	31.914395646224914	21.248529709302524
35	19.900320291897714	28.648357046593215	30.370676145112117	21.080646516396957
36	22.26410193101036	26.14907718897565	30.302584110915642	21.284236769098346
37	21.338761835197133	26.903362036151147	30.355523149148343	21.402352979503377
38	21.18382405892822	25.303617172309494	31.66088838135997	21.851670387402308
39	20.656148301321288	25.190366209547715	32.340522754784665	21.812962734346332
40	19.33322959853777	25.84400258565408	32.03636976225014	22.786398053558017
41	20.96471645251004	24.918619624222845	32.028846846290755	22.087817076976364
42	19.57111234564091	27.18155989698535	31.263074013490666	21.984253743883077
43	20.061023493787914	27.062136285231254	30.340991704645603	22.53584851633523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	287.0
1	542.0
2	797.0
3	1555.0
4	2313.0
5	2313.0
6	4218.5
7	6124.0
8	6987.0
9	7850.0
10	12460.0
11	17070.0
12	17070.0
13	33806.5
14	50543.0
15	81898.0
16	113253.0
17	106236.5
18	99220.0
19	99220.0
20	107385.5
21	115551.0
22	92967.5
23	70384.0
24	79130.0
25	87876.0
26	87876.0
27	95732.0
28	103588.0
29	111170.5
30	118753.0
31	130906.5
32	143060.0
33	143060.0
34	160778.5
35	178497.0
36	197227.5
37	215958.0
38	229182.0
39	242406.0
40	242406.0
41	251368.5
42	260331.0
43	273252.0
44	286173.0
45	307932.0
46	329691.0
47	329691.0
48	428805.0
49	527919.0
50	480465.0
51	433011.0
52	389957.5
53	346904.0
54	346904.0
55	302799.0
56	258694.0
57	217771.0
58	176848.0
59	158393.5
60	139939.0
61	139939.0
62	123692.5
63	107446.0
64	92307.5
65	77169.0
66	65204.5
67	53240.0
68	53240.0
69	45146.0
70	37052.0
71	30378.0
72	23704.0
73	19218.0
74	14732.0
75	14732.0
76	11719.0
77	8706.0
78	6828.5
79	4951.0
80	3842.5
81	2734.0
82	2734.0
83	2071.0
84	1408.0
85	1076.0
86	744.0
87	579.0
88	414.0
89	414.0
90	325.5
91	237.0
92	169.0
93	101.0
94	74.5
95	48.0
96	48.0
97	29.0
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4665744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.189108236093425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.77910772646453	27.029910058200706
2	13.858671788040294	10.585006338439305
3	5.910004296472264	6.770933812612695
4	3.055523233373822	4.667508299088443
5	1.6781148595229227	3.2042855001458808
6	1.0518036814466314	2.410046678033216
7	0.7125901577829113	1.904922786448255
8	0.5095166605676151	1.5566389518807626
9	0.3569617026225476	1.22688441878334
>10	1.7848707897277276	12.38905760091113
>50	0.16664726600667443	4.4240053124251855
>100	0.11744489540207602	8.72241857023032
>500	0.011088167314022365	2.9272884809835786
>1k	0.006472787900367844	5.056174676768593
>5k	6.191362339482273E-4	1.5203044650856419
>10k+	5.628511217711157E-4	5.604614049962894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	56943	1.2204484429492917	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	54187	1.161379621342277	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	44083	0.9448225191952239	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20826	0.44635968025678224	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	15740	0.33735241367721847	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15113	0.32391404243353256	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	14562	0.31210456467392983	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	13646	0.2924721116289278	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13345	0.2860208361195985	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12298	0.2635806850954532	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	8491	0.18198598122828855	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7356	0.15765974301204694	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7288	0.15620231199997256	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7163	0.15352321087483584	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6667	0.14289253761029325	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6433	0.13787726030403724	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5718	0.1225528018682551	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5560	0.11916641804608226	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5524	0.11839483692204288	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5288	0.1133366939977847	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5241	0.11232935197473329	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4889	0.10478500320634822	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4731	0.10139861938417538	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4702	0.10077706792314366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.143280900109393E-5	0.0
2	0.0	0.0	0.0	4.286561800218786E-5	0.0
3	0.0	0.0	0.0	4.286561800218786E-5	0.0
4	0.0	0.0	0.0	4.286561800218786E-5	0.0
5	0.0	0.0	0.0	4.286561800218786E-5	0.0
6	0.0	0.0	0.0	4.286561800218786E-5	0.0
7	0.0	0.0	0.0	4.286561800218786E-5	0.0
8	2.143280900109393E-5	0.0	0.0	6.429842700328179E-5	0.0
9	4.286561800218786E-5	0.0	0.0	2.3576089901203323E-4	0.0
10	4.286561800218786E-5	0.0	0.0	4.7152179802406645E-4	0.0
11	4.286561800218786E-5	0.0	0.0	9.001779780459451E-4	0.0
12	4.286561800218786E-5	0.0	0.0	0.0013502669670689177	0.0
13	6.429842700328179E-5	0.0	0.0	0.001543162248078763	0.0
14	6.429842700328179E-5	0.0	0.0	0.0018003559560918901	0.0
15	6.429842700328179E-5	0.0	0.0	0.0023576089901203323	0.0
16	6.429842700328179E-5	0.0	0.0	0.003043458878155338	0.0
17	6.429842700328179E-5	0.0	0.0	0.004500889890229725	0.0
18	6.429842700328179E-5	0.0	0.0	0.005208172587265825	0.0
19	8.573123600437572E-5	0.0	0.0	0.006408409891327085	0.0
20	8.573123600437572E-5	0.0	0.0	0.010566374837539307	0.0
21	8.573123600437572E-5	0.0	0.0	0.01772493304390468	0.0
22	8.573123600437572E-5	0.0	0.0	0.028055546982431956	0.0
23	8.573123600437572E-5	0.0	0.0	0.03412103192974154	0.0
24	8.573123600437572E-5	0.0	0.0	0.04260842429417473	0.0
25	8.573123600437572E-5	0.0	0.0	0.046980717330397895	0.0
26	8.573123600437572E-5	0.0	0.0	0.056454018908881416	0.0
27	8.573123600437572E-5	0.0	0.0	0.11271514253675298	0.0
28	8.573123600437572E-5	0.0	0.0	0.22581607563552566	0.0
29	8.573123600437572E-5	0.0	0.0	0.3636504703215607	0.0
30	8.573123600437572E-5	0.0	0.0	0.5912454691041772	0.0
31	8.573123600437572E-5	0.0	0.0	0.8929765542215775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14495	0.0	28.729563	1
GTATTGG	1705	0.0	18.228739	1
ATTGGAC	1765	0.0	17.294617	3
GTATCAA	24455	0.0	17.02106	2
TTAACGG	540	0.0	16.787037	35
GGACCCT	2745	0.0	15.366121	6
GTACTAG	495	0.0	15.323231	1
TTGGACC	2835	0.0	15.269841	4
GTATTAG	960	0.0	15.223959	1
TATACCG	305	0.0	15.163934	5
TGCGACG	265	0.0	14.6603775	22
ATTAGAG	915	0.0	14.557378	3
ACGTTTA	255	0.0	14.509804	26
TGGACCC	2855	0.0	14.450088	5
AACGGCC	670	0.0	14.358209	37
TACTCCG	1080	0.0	14.217592	5
TAACGGC	640	0.0	14.1640625	36
CTAATAC	920	0.0	14.076087	3
TCCGGTC	910	0.0	14.0274725	8
CGAATTA	345	0.0	13.405797	15
>>END_MODULE