FastQCFastQC Report
Wed 25 May 2016
SRR2088087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809378
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188891.0439499098585259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188461.0415734025725967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT153420.8479156925750174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69870.3861548001578443No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG57950.3202758074874349No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG55540.30695631316397126No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA54650.3020374957582108No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG50570.2794883103475338No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50290.27794081723111475No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49860.2755643099451856No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA31390.17348503187283143No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28730.15878384726685082No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28460.1572916217617325No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27470.15182012824296526No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA24620.13606885902227173No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.12921567522098754No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA23090.12761291449326784No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21910.12109133635978774No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG20040.11075629304656075No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19790.10937460276404377No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.10876665903973631No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC19220.10622434891990507No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18390.10163713718194872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.030.06251
GTATTGG6700.020.9850751
ATTGGAC6950.019.1654683
CAATGCG953.6087022E-617.52631619
GTATCAA82150.017.497872
GTACTAG2050.017.1463411
CGACGAG1005.881546E-616.6524
TGGACCC11650.015.87982755
CGCGATA700.002593265815.85714214
GTGCGCG1059.348949E-615.85714211
TCTATAC1751.3278623E-1015.8571423
GGACCCT11000.015.6409096
CTATCCT2250.015.6222224
TTGGACC11750.015.58723454
TGCGCGA855.366265E-415.23529412
TACCGAA1252.9608345E-614.7999997
TTAACGG1904.5656634E-1014.60526435
ACGGTGT1405.9998274E-714.53571429
TACGGCT2059.276846E-1114.4390244
GACCCTC11650.014.2918457
Produced by FastQC (version 0.11.5)