##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1809378
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.618172101130884	31.0	31.0	34.0	30.0	34.0
2	31.82230412882217	31.0	31.0	34.0	30.0	34.0
3	31.893909951375555	31.0	31.0	34.0	30.0	34.0
4	35.596234175501195	37.0	35.0	37.0	33.0	37.0
5	35.48336168561793	37.0	35.0	37.0	33.0	37.0
6	35.51625420448353	37.0	35.0	37.0	33.0	37.0
7	35.38428399151532	37.0	35.0	37.0	33.0	37.0
8	35.398803345680115	37.0	35.0	37.0	33.0	37.0
9	36.973411304879356	39.0	37.0	39.0	33.0	39.0
10	36.81631533046163	39.0	37.0	39.0	32.0	39.0
11	36.879932772477616	39.0	37.0	39.0	33.0	39.0
12	36.75481850669125	39.0	37.0	39.0	32.0	39.0
13	36.79105637406888	39.0	37.0	39.0	32.0	39.0
14	37.795010771657445	40.0	37.0	41.0	32.0	41.0
15	37.792587286901906	40.0	37.0	41.0	32.0	41.0
16	37.67021650534051	39.0	37.0	41.0	32.0	41.0
17	37.68996141215379	39.0	37.0	41.0	32.0	41.0
18	37.67592012282674	39.0	37.0	41.0	32.0	41.0
19	37.702526503583	39.0	37.0	41.0	32.0	41.0
20	37.6230931292411	39.0	37.0	41.0	32.0	41.0
21	37.53666066460408	39.0	36.0	41.0	32.0	41.0
22	37.44408741567544	39.0	36.0	41.0	32.0	41.0
23	37.25035177834593	39.0	36.0	41.0	31.0	41.0
24	37.12046515432375	39.0	36.0	41.0	31.0	41.0
25	36.97586408146888	39.0	36.0	40.0	31.0	41.0
26	37.11680146437063	39.0	36.0	41.0	31.0	41.0
27	37.05780881606828	39.0	36.0	41.0	31.0	41.0
28	36.965447794767044	39.0	36.0	41.0	31.0	41.0
29	36.85948928305749	39.0	35.0	40.0	30.0	41.0
30	36.65978419103139	39.0	35.0	40.0	30.0	41.0
31	36.491694936049846	39.0	35.0	40.0	30.0	41.0
32	36.35931076867299	38.0	35.0	40.0	30.0	41.0
33	36.19451546332497	38.0	35.0	40.0	30.0	41.0
34	36.06082753299753	38.0	35.0	40.0	30.0	41.0
35	35.86370122771472	38.0	35.0	40.0	29.0	41.0
36	35.74272153192976	38.0	35.0	40.0	27.0	41.0
37	35.58168331879795	38.0	34.0	40.0	27.0	41.0
38	35.48792236890246	38.0	34.0	40.0	26.0	41.0
39	35.398441342826096	38.0	34.0	40.0	26.0	41.0
40	35.21951853067739	38.0	34.0	40.0	25.0	41.0
41	35.09200399253224	38.0	34.0	40.0	24.0	41.0
42	34.89817937434853	38.0	34.0	40.0	24.0	41.0
43	34.55840349556588	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	2.0
15	11.0
16	26.0
17	55.0
18	115.0
19	235.0
20	547.0
21	1072.0
22	2083.0
23	3683.0
24	6151.0
25	9438.0
26	14262.0
27	20716.0
28	28130.0
29	37138.0
30	48062.0
31	60036.0
32	74714.0
33	93488.0
34	115752.0
35	143297.0
36	184550.0
37	250790.0
38	336729.0
39	378293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27859297504446	21.798209108323412	14.433910437730535	23.489287478901588
2	18.467561780899292	23.21173353494958	35.96882464581751	22.351880038333615
3	19.08926714042063	24.615199256319023	33.195274840304236	23.10025876295611
4	13.554934347604537	16.501250706043734	36.730633399986075	33.213181546365654
5	12.21137871688503	38.511024230426145	35.592120607192086	13.68547644549674
6	31.839283997042077	37.81139153897085	13.577372997792612	16.77195146619446
7	25.46344655456184	33.5988389380218	21.766430231825524	19.17128427559084
8	26.269856271050052	35.38221421947211	19.20168146180621	19.14624804767163
9	24.219704229851363	14.808072166236133	22.271078790612023	38.70114481330048
10	16.008705754132084	27.84669648906972	34.11879662513858	22.02580113165961
11	32.747994062047844	22.51746180179045	24.013003363586822	20.721540772574883
12	20.819530247410988	27.34320855012054	31.063437269603146	20.773823932865326
13	31.030387238045336	21.583328635586373	26.715092147688324	20.671191978679968
14	20.24015987814597	23.28076278146413	27.820389106090605	28.6586882342993
15	22.06023285349993	29.74889713481649	26.123507636325854	22.067362375357717
16	21.57923883235012	29.065734191528804	26.76129587073569	22.593731105385388
17	21.25763660219147	27.114455906947025	27.204984254257543	24.42292323660396
18	22.86780318982545	25.954886154247482	29.975218003092774	21.20209265283429
19	22.486954080352476	25.028822059293304	30.886249307773166	21.597974552581054
20	23.641881353702765	24.57181418144799	30.94732001826042	20.838984446588828
21	22.3750924350799	25.493401599886813	31.47794435435824	20.65356161067505
22	20.858936054268373	25.781622192819853	30.96279494942461	22.396646803487165
23	22.077586883448344	26.03601900763688	31.916271779583923	19.970122329330852
24	21.983853014682396	25.892102147809908	29.499363869793925	22.62468096771377
25	21.76228516097797	26.438698823573624	30.494567746485256	21.304448268963146
26	21.45698687615302	27.05587223896831	30.348661252651464	21.13847963222721
27	20.266025120234687	27.158448925542366	30.095811930950855	22.479714023272084
28	21.02794440962585	26.503251393572818	30.22801205718208	22.240792139619252
29	21.22447603541106	26.348446814319615	29.62869008023752	22.7983870700318
30	21.504406486649003	26.58405264129441	31.189613226202596	20.72192764585399
31	22.30423935739243	26.186070572318222	30.97694345791758	20.532746612371767
32	21.670540926218845	26.15915524561479	30.0829898451291	22.087313983037266
33	21.358555260426513	26.069013771583382	30.808432511061813	21.763998456928295
34	20.35500597442878	26.74068105172054	31.499001314263797	21.405311659586886
35	20.137251585904107	28.660512065472222	30.03087248767256	21.17136386095111
36	22.370007814840235	26.190823586890083	30.062927702226954	21.376240896042727
37	21.470251102865184	26.926932901803823	30.06198815283484	21.540827842496153
38	21.230279134597634	25.460351568329003	31.454731957611955	21.854637339461405
39	20.740165957583216	25.28576118423016	32.19675490693487	21.777317951251757
40	19.455028192008523	25.95311759068586	31.79407509099812	22.797779126307493
41	21.098576416868116	24.916297202685122	31.816182135518396	22.16894424492837
42	19.65570488864129	27.27257654287827	31.070843129517435	22.000875438963003
43	20.119013274174883	27.113184751887115	30.17180489648929	22.595997077448715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	207.5
2	293.0
3	520.5
4	748.0
5	748.0
6	1400.5
7	2053.0
8	2315.5
9	2578.0
10	4267.5
11	5957.0
12	5957.0
13	11571.5
14	17186.0
15	27814.0
16	38442.0
17	36985.5
18	35529.0
19	35529.0
20	39433.0
21	43337.0
22	36233.0
23	29129.0
24	33042.5
25	36956.0
26	36956.0
27	40622.0
28	44288.0
29	47331.5
30	50375.0
31	55377.5
32	60380.0
33	60380.0
34	67112.0
35	73844.0
36	81139.5
37	88435.0
38	92828.0
39	97221.0
40	97221.0
41	99060.5
42	100900.0
43	105099.0
44	109298.0
45	116495.5
46	123693.0
47	123693.0
48	160956.5
49	198220.0
50	180162.5
51	162105.0
52	146758.5
53	131412.0
54	131412.0
55	115573.5
56	99735.0
57	84437.0
58	69139.0
59	61992.5
60	54846.0
61	54846.0
62	48221.0
63	41596.0
64	36148.0
65	30700.0
66	26060.5
67	21421.0
68	21421.0
69	18179.0
70	14937.0
71	12476.5
72	10016.0
73	8153.5
74	6291.0
75	6291.0
76	5010.0
77	3729.0
78	2952.0
79	2175.0
80	1601.5
81	1028.0
82	1028.0
83	808.0
84	588.0
85	445.0
86	302.0
87	242.0
88	182.0
89	182.0
90	150.5
91	119.0
92	86.0
93	53.0
94	33.0
95	13.0
96	13.0
97	8.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1809378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.40692553515413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5018999426322	37.51628699355964
2	12.723596033194697	12.318203314364766
3	4.184963044247642	6.0774358335080265
4	1.8554740596946795	3.5927117856020194
5	0.9562235903376382	2.314392206621589
6	0.5727040840511423	1.663370637020538
7	0.3714785868884386	1.2587495405389053
8	0.2528606176841022	0.9792164072805932
9	0.19308333208173964	0.8411913430344188
>10	1.1674887456416174	10.740479016785638
>50	0.12408909295524959	4.191800805374676
>100	0.08399299604564969	7.688372599671009
>500	0.006645855883180343	2.2700202209776412
>1k	0.004468765162828161	3.7241843252969207
>5k	6.875023327427941E-4	1.8795918298458032
>10k+	3.4375116637139707E-4	2.9439931405177706
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18889	1.0439499098585259	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18846	1.0415734025725967	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15342	0.8479156925750174	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6987	0.3861548001578443	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5795	0.3202758074874349	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5554	0.30695631316397126	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5465	0.3020374957582108	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5057	0.2794883103475338	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5029	0.27794081723111475	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4986	0.2755643099451856	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3139	0.17348503187283143	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2873	0.15878384726685082	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2846	0.1572916217617325	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2747	0.15182012824296526	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2462	0.13606885902227173	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2338	0.12921567522098754	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2309	0.12761291449326784	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2191	0.12109133635978774	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2004	0.11075629304656075	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1979	0.10937460276404377	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.10876665903973631	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1922	0.10622434891990507	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1839	0.10163713718194872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.526761130067902E-5	0.0
5	0.0	0.0	0.0	5.526761130067902E-5	0.0
6	0.0	0.0	0.0	5.526761130067902E-5	0.0
7	0.0	0.0	0.0	1.1053522260135804E-4	0.0
8	0.0	0.0	0.0	1.1053522260135804E-4	0.0
9	0.0	0.0	0.0	3.316056678040741E-4	0.0
10	0.0	0.0	0.0	4.974085017061112E-4	0.0
11	0.0	0.0	0.0	0.0010500846147129014	0.0
12	0.0	0.0	0.0	0.0015474931164190125	0.0
13	0.0	0.0	0.0	0.0016027607277196915	0.0
14	0.0	0.0	0.0	0.0018238311729224077	0.0
15	0.0	0.0	0.0	0.0023765072859291978	0.0
16	0.0	0.0	0.0	0.003316056678040741	0.0
17	0.0	0.0	0.0	0.004310873681452963	0.0
18	0.0	0.0	0.0	0.005139887850963149	0.0
19	0.0	0.0	0.0	0.006466310522179445	0.0
20	0.0	0.0	0.0	0.011661465984443273	0.0
21	0.0	0.0	0.0	0.02138856557336278	0.0
22	0.0	0.0	0.0	0.03343690483691081	0.0
23	0.0	0.0	0.0	0.04045589147209704	0.0
24	0.0	0.0	0.0	0.050348793894918586	0.0
25	0.0	0.0	0.0	0.054991273244175624	0.0
26	0.0	0.0	0.0	0.06488417566699717	0.0
27	0.0	0.0	0.0	0.12650756226725426	0.0
28	0.0	0.0	0.0	0.2524071808102011	0.0
29	5.526761130067902E-5	0.0	0.0	0.407045957229501	0.0
30	5.526761130067902E-5	0.0	0.0	0.6412700939217787	0.0
31	1.1053522260135804E-4	0.0	0.0	0.9419258993974725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4800	0.0	30.0625	1
GTATTGG	670	0.0	20.985075	1
ATTGGAC	695	0.0	19.165468	3
CAATGCG	95	3.6087022E-6	17.526316	19
GTATCAA	8215	0.0	17.49787	2
GTACTAG	205	0.0	17.146341	1
CGACGAG	100	5.881546E-6	16.65	24
TGGACCC	1165	0.0	15.8798275	5
CGCGATA	70	0.0025932658	15.857142	14
GTGCGCG	105	9.348949E-6	15.857142	11
TCTATAC	175	1.3278623E-10	15.857142	3
GGACCCT	1100	0.0	15.640909	6
CTATCCT	225	0.0	15.622222	4
TTGGACC	1175	0.0	15.5872345	4
TGCGCGA	85	5.366265E-4	15.235294	12
TACCGAA	125	2.9608345E-6	14.799999	7
TTAACGG	190	4.5656634E-10	14.605264	35
ACGGTGT	140	5.9998274E-7	14.535714	29
TACGGCT	205	9.276846E-11	14.439024	4
GACCCTC	1165	0.0	14.291845	7
>>END_MODULE