FastQCFastQC Report
Wed 25 May 2016
SRR2088086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661018
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121401.8365611829027346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118531.7931433032080821No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69651.0536778121019397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40180.6078503157251391No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26810.40558653470858586No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG26370.3989301350341443No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG24540.37124556366089884No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17650.2670123960315755No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16640.25173293314251655No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15640.23660475206424028No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.22072016193205027No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.214517607689957No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA12110.1832022728579252No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11270.17049460075217315No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC10410.1574843650248556No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA10030.15173565621511065No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG9380.1419023385142311No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8980.13585106608292058No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC8820.1334305571103964No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT7850.11875622146446844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7600.11497417619489939No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.11467161257333386No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG7450.11270494903315796No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7230.10937674919593718No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7150.10816649470967508No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.10362804038619221No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.1024177858999301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCG200.001841370437.010
GGTATCA25800.031.1201551
GTATTGG1151.3460522E-1020.9130441
GTATCAA39950.020.0513132
TATACCG656.8964604E-519.9230775
GGACCCT2250.019.7333346
TTGGACT1002.870329E-718.54
ATTGGAC1351.1496013E-917.8148143
TAATAGT752.0655918E-417.2666664
TTGGACC2250.017.2666664
CTAATAC752.0655918E-417.2666663
GACCCTC2550.016.6862747
CGACCGC904.4413588E-516.44444510
CTAAACG803.3798462E-416.187526
CGCCGGA803.3798462E-416.187514
TAGTACA803.3798462E-416.18754
TAAGCCG700.002590930815.85714310
GCGTCGG700.002590930815.85714311
CCGACCG1059.33101E-615.8571429
CTAGGCA957.0524235E-515.5789484
Produced by FastQC (version 0.11.5)