##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661018
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93538148734225	31.0	31.0	31.0	28.0	34.0
2	31.14236979930955	31.0	31.0	34.0	28.0	34.0
3	31.0969035638969	31.0	31.0	34.0	28.0	34.0
4	34.88688356444151	35.0	35.0	37.0	32.0	37.0
5	34.70958430784033	35.0	35.0	37.0	32.0	37.0
6	34.862953807611895	35.0	35.0	37.0	32.0	37.0
7	34.70538018631868	35.0	35.0	37.0	31.0	37.0
8	34.806282128474564	35.0	35.0	37.0	32.0	37.0
9	36.199226042256036	38.0	35.0	39.0	32.0	39.0
10	35.9427549627998	37.0	35.0	39.0	30.0	39.0
11	36.08705663083305	37.0	35.0	39.0	31.0	39.0
12	35.874619148041354	37.0	35.0	39.0	30.0	39.0
13	35.97164978865931	37.0	35.0	39.0	30.0	39.0
14	36.69374510225138	38.0	36.0	40.0	31.0	41.0
15	36.77754312288016	38.0	36.0	40.0	31.0	41.0
16	36.549295178043565	38.0	35.0	40.0	30.0	41.0
17	36.661991050168076	38.0	36.0	40.0	31.0	41.0
18	36.64960560831929	38.0	36.0	40.0	31.0	41.0
19	36.70496113570281	38.0	35.0	40.0	30.0	41.0
20	36.63214617453685	38.0	35.0	40.0	30.0	41.0
21	36.54966279284376	38.0	35.0	40.0	30.0	41.0
22	36.425251657292236	38.0	35.0	40.0	30.0	41.0
23	36.18399498954643	38.0	34.0	40.0	29.0	41.0
24	36.00594688798187	38.0	34.0	40.0	29.0	41.0
25	35.841800072010145	38.0	34.0	40.0	27.0	41.0
26	35.92062092106418	38.0	34.0	40.0	29.0	41.0
27	35.79173940800402	38.0	34.0	40.0	29.0	41.0
28	35.662998587027886	38.0	34.0	40.0	27.0	41.0
29	35.55830098423946	38.0	34.0	40.0	27.0	41.0
30	35.246005403786285	38.0	34.0	40.0	27.0	41.0
31	34.954586713221126	37.0	33.0	40.0	26.0	41.0
32	34.773989513144876	37.0	33.0	40.0	25.0	41.0
33	34.54286418826719	37.0	33.0	40.0	25.0	41.0
34	34.32994260368099	37.0	33.0	40.0	24.0	41.0
35	34.09409577348877	37.0	33.0	40.0	24.0	41.0
36	33.9612310103507	37.0	33.0	40.0	23.0	41.0
37	33.711865637546936	36.0	32.0	40.0	22.0	41.0
38	33.52629882998647	36.0	32.0	40.0	21.0	41.0
39	33.341061816773525	36.0	31.0	40.0	20.0	41.0
40	33.103674937747535	36.0	31.0	40.0	18.0	41.0
41	32.874995839750206	36.0	31.0	40.0	16.0	41.0
42	32.572004090660165	36.0	31.0	39.0	15.0	41.0
43	32.17344157042622	36.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	5.0
14	2.0
15	3.0
16	25.0
17	65.0
18	118.0
19	280.0
20	599.0
21	1102.0
22	1939.0
23	3103.0
24	4851.0
25	6928.0
26	9575.0
27	13130.0
28	16896.0
29	21067.0
30	25926.0
31	31044.0
32	36835.0
33	44339.0
34	52394.0
35	62478.0
36	79432.0
37	97415.0
38	102887.0
39	48578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.76311688940392	25.05483965640875	17.04356008459679	20.13848336959054
2	21.862944730703248	26.036507326578096	31.729695711765793	20.370852230952863
3	22.171710906510867	24.70855559152701	33.56413894931757	19.555594552644557
4	16.856727048280078	19.06438251303293	33.121639652777986	30.957250785909007
5	11.659440438838278	41.12369103413221	33.99589723729157	13.220971289737948
6	31.677503487045737	38.095483027693646	12.500567306790437	17.72644617847018
7	23.868790259871894	35.65621511063239	20.817738700005144	19.657255929490574
8	27.478828110580956	35.962106932035134	18.173030083900894	18.38603487348302
9	23.951843973991632	14.809279021146171	22.57714615940867	38.661730845453526
10	16.958085861504525	29.941695990124323	32.21712570610785	20.883092442263298
11	32.84660932077493	22.71239209824846	23.26003225328206	21.180966327694556
12	19.863301755776696	29.534899200929477	30.895679088920424	19.706119954373406
13	32.689276237560854	21.532091410521346	27.06492107627931	18.713711275638484
14	19.211579714924557	24.680417174721413	29.119328066709226	26.9886750436448
15	22.009536805351747	30.913530342592786	26.603208989770323	20.47372386228514
16	20.318962569854378	29.17152029142928	28.538859758735768	21.970657379980576
17	20.84996172570187	28.207855156743083	28.30891140634597	22.633271711209073
18	22.053862375911095	25.632584891788117	31.160876103222606	21.15267662907818
19	21.780042298394296	25.05847041986754	33.303480389338866	19.8580068923993
20	22.547494924495247	24.429591932443596	33.39288793951148	19.630025203549675
21	21.55206060954467	25.282821345258373	34.27107885110542	18.89403919409154
22	19.4560511211495	25.827587145887097	33.634484991331554	21.08187674163185
23	20.979307673921134	25.888704997443334	34.512827184736274	18.619160143899258
24	20.577805748103685	25.832276882021365	32.38353569796889	21.206381671906062
25	20.488549479741852	26.803808670868268	32.915593826491865	19.792048022898015
26	20.469639253394007	27.577766414832876	32.59381741495693	19.358776916816183
27	19.238205313622323	27.351146262280302	32.44586380401139	20.96478462008599
28	19.830171039215273	27.12422354610616	32.67626600183354	20.369339412845036
29	20.030922002123997	26.060561134492556	31.924092838621643	21.984424024761807
30	20.47084950788027	26.814549679433842	33.70180539712988	19.012795415556006
31	21.203507317501188	26.787016389871383	33.04115772944156	18.968318563185875
32	20.79519771019851	26.46569382376879	32.34011781827424	20.39899064775846
33	20.452544408775555	26.659485823381512	32.62150198633017	20.266467781512755
34	19.39644608770109	27.19714137890345	33.500751870599586	19.905660662795867
35	18.659098541945905	29.116302430493572	32.58126102466196	19.64333800289856
36	21.508491448039237	26.320614567228123	32.15222580928205	20.018668175450593
37	20.417900874106305	27.4494794392891	31.986572226474923	20.14604746012968
38	20.447854672641288	25.61775927433141	33.50438263405838	20.430003418968923
39	19.610661131769483	25.65709254513493	34.26911218756524	20.463134135530346
40	18.284978623880136	26.30034280458323	33.31966754309262	22.095011028444006
41	20.119573143242693	25.30233669884935	33.40741099334662	21.170679164561328
42	19.099631174945312	27.52254855389717	32.172951417359286	21.20486885379823
43	19.25272836745747	27.573833087752526	31.418509026985646	21.754929517804356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	82.0
2	92.0
3	156.0
4	220.0
5	220.0
6	476.0
7	732.0
8	957.0
9	1182.0
10	2002.5
11	2823.0
12	2823.0
13	6257.0
14	9691.0
15	16526.0
16	23361.0
17	20923.5
18	18486.0
19	18486.0
20	21461.5
21	24437.0
22	19190.5
23	13944.0
24	14870.5
25	15797.0
26	15797.0
27	16547.5
28	17298.0
29	17837.5
30	18377.0
31	19735.0
32	21093.0
33	21093.0
34	23329.5
35	25566.0
36	27871.0
37	30176.0
38	31274.0
39	32372.0
40	32372.0
41	33357.0
42	34342.0
43	35835.0
44	37328.0
45	40485.0
46	43642.0
47	43642.0
48	59554.5
49	75467.0
50	67627.0
51	59787.0
52	51978.0
53	44169.0
54	44169.0
55	38065.0
56	31961.0
57	27064.0
58	22167.0
59	19604.0
60	17041.0
61	17041.0
62	14852.0
63	12663.0
64	10985.5
65	9308.0
66	7693.0
67	6078.0
68	6078.0
69	5161.5
70	4245.0
71	3554.0
72	2863.0
73	2358.5
74	1854.0
75	1854.0
76	1435.0
77	1016.0
78	813.5
79	611.0
80	479.5
81	348.0
82	348.0
83	260.5
84	173.0
85	139.0
86	105.0
87	81.0
88	57.0
89	57.0
90	53.0
91	49.0
92	32.0
93	15.0
94	12.5
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	661018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.626467663310926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.622010857726	44.30726593539568
2	10.153286782249346	10.889698106092336
3	2.953762041479939	4.751994738076176
4	1.244434619120909	2.6693853144556834
5	0.6808613005683993	1.8256093259065547
6	0.41534062723184406	1.3363950429304658
7	0.2999177615537854	1.125847109913167
8	0.22226997315694094	0.9535642822420545
9	0.15696749461542903	0.7575851046766714
>10	1.0928881641149504	11.292391213309049
>50	0.09269537361603614	3.481769359767887
>100	0.05369547787760122	5.680796490613528
>500	0.007347802235880957	2.592306690106053
>1k	0.0036739011179404784	3.6436268601888178
>5k	2.8260777830311373E-4	1.0555636420104448
>10k+	5.652155566062275E-4	3.636200784315377
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12140	1.8365611829027346	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11853	1.7931433032080821	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6965	1.0536778121019397	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4018	0.6078503157251391	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2681	0.40558653470858586	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2637	0.3989301350341443	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2454	0.37124556366089884	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1765	0.2670123960315755	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1664	0.25173293314251655	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1564	0.23660475206424028	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1459	0.22072016193205027	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.214517607689957	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1211	0.1832022728579252	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1127	0.17049460075217315	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1041	0.1574843650248556	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1003	0.15173565621511065	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	938	0.1419023385142311	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	898	0.13585106608292058	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	882	0.1334305571103964	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	785	0.11875622146446844	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	760	0.11497417619489939	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	758	0.11467161257333386	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	745	0.11270494903315796	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	723	0.10937674919593718	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	715	0.10816649470967508	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	685	0.10362804038619221	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	677	0.1024177858999301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.5128181078276234E-4	0.0	0.0	0.0	0.0
7	1.5128181078276234E-4	0.0	0.0	0.0	0.0
8	3.025636215655247E-4	0.0	0.0	0.0	0.0
9	3.025636215655247E-4	0.0	0.0	0.0	0.0
10	3.025636215655247E-4	0.0	0.0	3.025636215655247E-4	0.0
11	3.025636215655247E-4	0.0	0.0	3.025636215655247E-4	0.0
12	3.025636215655247E-4	0.0	0.0	0.0010589726754793363	0.0
13	3.025636215655247E-4	0.0	0.0	0.0012102544862620987	0.0
14	3.025636215655247E-4	0.0	0.0	0.0013615362970448611	0.0
15	3.025636215655247E-4	0.0	0.0	0.0018153817293931481	0.0
16	3.025636215655247E-4	0.0	0.0	0.0024205089725241975	0.0
17	3.025636215655247E-4	0.0	0.0	0.003933327080351821	0.0
18	3.025636215655247E-4	0.0	0.0	0.004689736134265632	0.0
19	3.025636215655247E-4	0.0	0.0	0.005748708809744969	0.0
20	3.025636215655247E-4	0.0	0.0	0.009379472268531265	0.0
21	3.025636215655247E-4	0.0	0.0	0.013312799348883086	0.0
22	3.025636215655247E-4	0.0	0.0	0.023297398860545403	0.0
23	3.025636215655247E-4	0.0	0.0	0.0269281623193317	0.0
24	3.025636215655247E-4	0.0	0.0	0.034340971047687054	0.0
25	3.025636215655247E-4	0.0	0.0	0.0397871162358665	0.0
26	3.025636215655247E-4	0.0	0.0	0.04886402488283224	0.0
27	3.025636215655247E-4	0.0	0.0	0.1084690583312406	0.0
28	3.025636215655247E-4	0.0	0.0	0.21360991682526043	0.0
29	3.025636215655247E-4	0.0	0.0	0.3464353466925258	0.0
30	3.025636215655247E-4	0.0	0.0	0.5668529450030105	0.0
31	3.025636215655247E-4	0.0	0.0	0.8290243230895377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCCG	20	0.0018413704	37.0	10
GGTATCA	2580	0.0	31.120155	1
GTATTGG	115	1.3460522E-10	20.913044	1
GTATCAA	3995	0.0	20.051313	2
TATACCG	65	6.8964604E-5	19.923077	5
GGACCCT	225	0.0	19.733334	6
TTGGACT	100	2.870329E-7	18.5	4
ATTGGAC	135	1.1496013E-9	17.814814	3
TAATAGT	75	2.0655918E-4	17.266666	4
TTGGACC	225	0.0	17.266666	4
CTAATAC	75	2.0655918E-4	17.266666	3
GACCCTC	255	0.0	16.686274	7
CGACCGC	90	4.4413588E-5	16.444445	10
CTAAACG	80	3.3798462E-4	16.1875	26
CGCCGGA	80	3.3798462E-4	16.1875	14
TAGTACA	80	3.3798462E-4	16.1875	4
TAAGCCG	70	0.0025909308	15.857143	10
GCGTCGG	70	0.0025909308	15.857143	11
CCGACCG	105	9.33101E-6	15.857142	9
CTAGGCA	95	7.0524235E-5	15.578948	4
>>END_MODULE