FastQCFastQC Report
Wed 25 May 2016
SRR2088085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812004
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT179280.9894017894000234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177330.9786402237522653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT131680.7267092125624447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.37130160860572053No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG47230.26065063874031186No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG45070.24873013525356455No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA45050.24861976022127985No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA40040.22097081463396331No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG38450.21219599956732987No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35330.19497749453091714No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA24410.1347127269034726No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.12395116125571468No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC20630.1138518458016649No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20040.11059578234926634No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA19680.1086090317681418No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.10662228118701726No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18470.10193134231491763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.029.9421981
TAGGGCG555.144666E-420.1818185
CAATGCG1205.178663E-918.519
GTATCAA73900.017.5737482
GTATTGG3800.017.5263161
CGTCGAG904.4482327E-516.44444510
TGCGACG1352.2215318E-816.44444322
CCGAGTT1708.54925E-1116.32352813
GACGTCG957.063268E-515.57894836
ATGCGAC1853.0559022E-1015.021
TAATACT4450.014.9662934
GTGGTAT6450.014.6279071
AATGCGA1651.6467311E-814.57575720
GTACTAG2600.014.2307691
GCGACGA1601.7849197E-713.87523
GGCGTCG800.006300469413.8758
GCGTCGG800.006300469413.8759
AAGACCG2405.456968E-1213.8749995
TATACGG1203.302424E-513.8749992
CGAGTTC1907.1322575E-913.63157914
Produced by FastQC (version 0.11.5)