##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1812004
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.408999097132234	31.0	31.0	34.0	30.0	34.0
2	31.627806009258258	31.0	31.0	34.0	30.0	34.0
3	31.693375952812467	31.0	31.0	34.0	30.0	34.0
4	35.42188207090051	37.0	35.0	37.0	33.0	37.0
5	35.289599802207945	37.0	35.0	37.0	33.0	37.0
6	35.32138891525626	37.0	35.0	37.0	33.0	37.0
7	35.18707740159514	37.0	35.0	37.0	32.0	37.0
8	35.220342780700264	37.0	35.0	37.0	32.0	37.0
9	36.745928265059014	39.0	35.0	39.0	32.0	39.0
10	36.547066673142005	38.0	35.0	39.0	32.0	39.0
11	36.613476570691894	38.0	35.0	39.0	32.0	39.0
12	36.47052379575321	38.0	35.0	39.0	32.0	39.0
13	36.50069922582952	38.0	35.0	39.0	32.0	39.0
14	37.45997139079163	39.0	36.0	41.0	32.0	41.0
15	37.46508396228706	39.0	36.0	41.0	32.0	41.0
16	37.33112509685409	39.0	36.0	41.0	32.0	41.0
17	37.33635301025826	39.0	36.0	41.0	32.0	41.0
18	37.30510914986942	39.0	36.0	40.0	32.0	41.0
19	37.33932209862672	39.0	36.0	40.0	31.0	41.0
20	37.248551327701264	39.0	36.0	40.0	31.0	41.0
21	37.15786278617487	39.0	36.0	40.0	31.0	41.0
22	37.034665486389656	39.0	36.0	40.0	31.0	41.0
23	36.81801530239448	39.0	36.0	40.0	30.0	41.0
24	36.66069225012748	39.0	35.0	40.0	30.0	41.0
25	36.51175990781477	38.0	35.0	40.0	30.0	41.0
26	36.67646318661548	39.0	35.0	40.0	30.0	41.0
27	36.59814934183368	39.0	35.0	40.0	30.0	41.0
28	36.523164408025586	39.0	35.0	40.0	30.0	41.0
29	36.436369897638194	39.0	35.0	40.0	30.0	41.0
30	36.20782404453853	38.0	35.0	40.0	30.0	41.0
31	36.028754351535646	38.0	35.0	40.0	29.0	41.0
32	35.85660296555637	38.0	35.0	40.0	28.0	41.0
33	35.692282136242525	38.0	34.0	40.0	27.0	41.0
34	35.550062251518206	38.0	34.0	40.0	27.0	41.0
35	35.354430233045846	38.0	34.0	40.0	26.0	41.0
36	35.263424363301624	38.0	34.0	40.0	26.0	41.0
37	35.092606859587505	38.0	34.0	40.0	25.0	41.0
38	35.00487636892634	38.0	33.0	40.0	25.0	41.0
39	34.909168522806794	38.0	33.0	40.0	24.0	41.0
40	34.74276215725793	38.0	33.0	40.0	24.0	41.0
41	34.601951761695894	38.0	33.0	40.0	23.0	41.0
42	34.39843510279227	38.0	33.0	40.0	23.0	41.0
43	34.0694970872029	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	3.0
15	14.0
16	22.0
17	60.0
18	187.0
19	417.0
20	902.0
21	1694.0
22	3231.0
23	5289.0
24	8485.0
25	12720.0
26	18203.0
27	25335.0
28	33789.0
29	43682.0
30	54567.0
31	67460.0
32	82090.0
33	100521.0
34	122631.0
35	149921.0
36	190526.0
37	254157.0
38	340756.0
39	295337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6030030838784	22.524177650821965	14.476402921847855	23.396416343451783
2	19.523080523001052	23.65977117048307	34.43524407230889	22.38190423420699
3	19.718223580080398	24.787252125271248	32.598051659930114	22.896472634718247
4	14.495994490078386	17.069498742828383	35.948265014867516	32.486241752225716
5	12.5016280317262	38.85830274105355	35.15803497122523	13.482034255995021
6	32.421396420758455	38.04837075414845	13.440091743726834	16.090141081366266
7	25.992768227884707	33.36135019569493	21.53521736155108	19.11066421486928
8	26.630073664296543	35.37955766102062	18.982187677289897	19.00818099739294
9	24.613577011971277	14.725574557230559	21.69068059452407	38.97016783627409
10	16.26387138218238	28.206449875386593	33.79308213447653	21.73659660795451
11	33.50511367524575	22.710104392705535	23.10265319502606	20.682128737022655
12	20.852602974386368	27.557279123004143	30.696124291116355	20.89399361149313
13	31.109202849441836	21.541674300939732	26.063904936192195	21.28521791342624
14	20.46562811119622	23.06435305882327	27.523283613060457	28.94673521692005
15	22.657014002176595	29.903024496634668	25.28311195781025	22.15684954337849
16	22.191783241096598	28.620632184034918	26.359544460166756	22.828040114701732
17	21.7420601720526	27.33448712033748	26.98664020609226	23.936812501517657
18	22.846693495157847	26.07416981419467	29.397396473738468	21.681740216909013
19	22.787256540272537	25.256732325094205	30.3211251189291	21.63488601570416
20	23.576769146204978	25.023896194489637	30.24275884600696	21.156575813298424
21	22.585270231191544	25.680848386648155	30.653133216041468	21.080748166118838
22	21.429919580751477	26.042712929993534	30.200595583674207	22.326771905580785
23	22.439685563608027	26.167105591378387	30.8400533332156	20.55315551179799
24	22.35867028991106	26.140229271017063	28.759152849552212	22.741947589519672
25	22.178814174803147	26.484599371745315	29.83508866426343	21.501497789188104
26	22.058836514709682	27.015172151937854	29.580894964911774	21.34509636844069
27	21.186377072015294	27.002644585773538	29.308268635168577	22.50270970704259
28	21.601166443341185	26.567491020991124	29.712793128491988	22.118549407175703
29	21.892777278637354	26.28399275056788	29.169361657038284	22.65386831375648
30	21.995922746307404	26.652590170882622	30.248498347685764	21.102988735124207
31	22.648294374626104	26.32610082538449	30.05826697954309	20.967337820446314
32	21.933505665550406	26.41362822598626	29.398334661512887	22.25453144695045
33	21.833340323752047	26.293264253279798	30.000320087593625	21.873075335374537
34	21.048904969304704	26.691552557279124	30.62901627148726	21.630526201928916
35	20.824733278734485	28.27195745704756	29.47278262078892	21.430526643429044
36	22.70811764212441	26.33984251690394	29.314560012008805	21.63747982896285
37	21.83582376197845	26.846187977509985	29.52603857386628	21.791949686645285
38	21.68196096697358	25.69883951691056	30.575760318409895	22.043439197705965
39	21.20249182672886	25.559159913554275	31.284754338290643	21.953593921426222
40	20.230198167332965	26.024887362279554	30.970185496279257	22.774728974108225
41	21.46501884101801	25.15242791958517	31.03033988887442	22.352213350522405
42	20.296478374219927	26.945580694082356	30.363895443939416	22.394045487758305
43	20.788585455661245	26.71760106489831	29.70639137661948	22.787422102820965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	206.5
2	298.0
3	448.5
4	599.0
5	599.0
6	1197.5
7	1796.0
8	2136.0
9	2476.0
10	3938.0
11	5400.0
12	5400.0
13	10942.5
14	16485.0
15	26322.0
16	36159.0
17	34147.5
18	32136.0
19	32136.0
20	36213.5
21	40291.0
22	33785.5
23	27280.0
24	30734.5
25	34189.0
26	34189.0
27	38196.0
28	42203.0
29	45594.0
30	48985.0
31	54102.0
32	59219.0
33	59219.0
34	66921.5
35	74624.0
36	81236.5
37	87849.0
38	93302.0
39	98755.0
40	98755.0
41	101956.5
42	105158.0
43	109286.5
44	113415.0
45	119113.0
46	124811.0
47	124811.0
48	153949.5
49	183088.0
50	168423.0
51	153758.0
52	140522.0
53	127286.0
54	127286.0
55	114530.5
56	101775.0
57	89434.0
58	77093.0
59	70451.0
60	63809.0
61	63809.0
62	56481.5
63	49154.0
64	42737.5
65	36321.0
66	30209.0
67	24097.0
68	24097.0
69	20302.5
70	16508.0
71	13522.5
72	10537.0
73	8729.0
74	6921.0
75	6921.0
76	5469.5
77	4018.0
78	3223.0
79	2428.0
80	1869.5
81	1311.0
82	1311.0
83	1051.0
84	791.0
85	603.0
86	415.0
87	317.0
88	219.0
89	219.0
90	186.5
91	154.0
92	102.5
93	51.0
94	34.5
95	18.0
96	18.0
97	11.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1812004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.35208765465433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04230390979376	39.29592927237108
2	12.331352001673197	12.41818633777292
3	4.090877127699817	6.179526111550851
4	1.8559620091847477	3.7380624708071517
5	0.9214668012328848	2.3198888573266077
6	0.5671258375272874	1.7133581929435937
7	0.36178365358882597	1.2751593566267965
8	0.25779133847925406	1.0384265657374452
9	0.19263454606198022	0.8729596393764638
>10	1.1783642082082197	11.486088603546337
>50	0.11353882151953737	3.9603382313076105
>100	0.07770377107023739	7.283240335867126
>500	0.005479502385741479	2.016585145804297
>1k	0.003178111383730058	3.336566901797856
>5k	1.0959004771482959E-4	0.3712569396900071
>10k+	3.2877014314448876E-4	2.694427037473745
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17928	0.9894017894000234	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17733	0.9786402237522653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13168	0.7267092125624447	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6728	0.37130160860572053	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4723	0.26065063874031186	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4507	0.24873013525356455	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4505	0.24861976022127985	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4004	0.22097081463396331	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3845	0.21219599956732987	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3533	0.19497749453091714	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2441	0.1347127269034726	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2246	0.12395116125571468	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2063	0.1138518458016649	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2004	0.11059578234926634	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1968	0.1086090317681418	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1932	0.10662228118701726	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1847	0.10193134231491763	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.518751614234847E-5	0.0	0.0	0.0	0.0
7	5.518751614234847E-5	0.0	0.0	0.0	0.0
8	5.518751614234847E-5	0.0	0.0	0.0	0.0
9	5.518751614234847E-5	0.0	0.0	1.1037503228469694E-4	0.0
10	5.518751614234847E-5	0.0	0.0	2.2075006456939388E-4	0.0
11	5.518751614234847E-5	0.0	0.0	3.311250968540908E-4	0.0
12	5.518751614234847E-5	0.0	0.0	5.518751614234847E-4	0.0
13	5.518751614234847E-5	0.0	0.0	6.622501937081816E-4	0.0
14	5.518751614234847E-5	0.0	0.0	8.278127421352271E-4	0.0
15	5.518751614234847E-5	0.0	0.0	9.933752905622725E-4	0.0
16	5.518751614234847E-5	0.0	0.0	0.0014900629358434087	0.0
17	5.518751614234847E-5	0.0	0.0	0.002317875677978636	0.0
18	5.518751614234847E-5	0.0	0.0	0.002759375807117424	0.0
19	5.518751614234847E-5	0.0	0.0	0.0038079386138220447	0.0
20	5.518751614234847E-5	0.0	0.0	0.005629126646519544	0.0
21	5.518751614234847E-5	0.0	0.0	0.010099315454049771	0.0
22	5.518751614234847E-5	0.0	0.0	0.017880755230120903	0.0
23	5.518751614234847E-5	0.0	0.0	0.02168869384394295	0.0
24	5.518751614234847E-5	0.0	0.0	0.02781450813574363	0.0
25	5.518751614234847E-5	0.0	0.0	0.032339884459416206	0.0
26	5.518751614234847E-5	0.0	0.0	0.0398453866547756	0.0
27	5.518751614234847E-5	0.0	0.0	0.08377464950408497	0.0
28	5.518751614234847E-5	0.0	0.0	0.1744477385259635	0.0
29	5.518751614234847E-5	0.0	0.0	0.2800766444224185	0.0
30	5.518751614234847E-5	0.0	0.0	0.44861931872115074	0.0
31	5.518751614234847E-5	0.0	0.0	0.6832214498422741	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4325	0.0	29.942198	1
TAGGGCG	55	5.144666E-4	20.181818	5
CAATGCG	120	5.178663E-9	18.5	19
GTATCAA	7390	0.0	17.573748	2
GTATTGG	380	0.0	17.526316	1
CGTCGAG	90	4.4482327E-5	16.444445	10
TGCGACG	135	2.2215318E-8	16.444443	22
CCGAGTT	170	8.54925E-11	16.323528	13
GACGTCG	95	7.063268E-5	15.578948	36
ATGCGAC	185	3.0559022E-10	15.0	21
TAATACT	445	0.0	14.966293	4
GTGGTAT	645	0.0	14.627907	1
AATGCGA	165	1.6467311E-8	14.575757	20
GTACTAG	260	0.0	14.230769	1
GCGACGA	160	1.7849197E-7	13.875	23
GGCGTCG	80	0.0063004694	13.875	8
GCGTCGG	80	0.0063004694	13.875	9
AAGACCG	240	5.456968E-12	13.874999	5
TATACGG	120	3.302424E-5	13.874999	2
CGAGTTC	190	7.1322575E-9	13.631579	14
>>END_MODULE