FastQCFastQC Report
Wed 25 May 2016
SRR2088084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577641
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT446841.2489794252693325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT442971.238162241544079No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT352740.9859569476087735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175620.4908821203692601No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA130660.3652127197782002No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG130350.3643462270250145No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG127650.3567993546585585No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG115860.32384467865836736No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA114250.31934450661762875No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA106380.2973467712383663No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA70470.1969733687645015No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA63330.17701608406209568No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60980.17044750996536545No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC57960.16200619346658873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53760.1502666142298794No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53680.15004300319679922No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51200.14311106117131372No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG50320.14065133980743177No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49160.1374089798277692No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG47420.13254543985827533No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG43840.12253884612793739No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.11513173065715648No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41140.11499197376148138No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38690.10814388587340093No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38570.10780846932378067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110250.030.1201821
GCCGTAT603.7291193E-521.58333430
TGCGACG1600.020.812522
GTATTGG13100.020.3358761
GTCTAAC2150.018.0697671
GTATCAA186500.017.805632
ATTGGAC15300.017.290853
TTAACGG4000.016.6535
GGACCCT21200.016.405666
CTAATAC8400.016.2976193
GTATGCG803.385064E-416.187533
TTGGACC23350.016.162744
CAATGCG2100.015.85714219
TCTAACG1557.223207E-915.5161292
TGGACCC24150.014.8612845
CCATGCG1252.9627936E-614.7999999
TAATACT9050.014.7182324
GACCCTC22700.014.5881057
CTAGTAC4600.014.478263
GTATTAG7350.014.3469381
Produced by FastQC (version 0.11.5)