##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3577641
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.592766294885372	31.0	31.0	34.0	30.0	34.0
2	31.79113639406525	31.0	31.0	34.0	30.0	34.0
3	31.855690942718958	31.0	31.0	34.0	30.0	34.0
4	35.55543303534368	37.0	35.0	37.0	33.0	37.0
5	35.4561944588627	37.0	35.0	37.0	33.0	37.0
6	35.491171137629514	37.0	35.0	37.0	33.0	37.0
7	35.35431363851208	37.0	35.0	37.0	33.0	37.0
8	35.39545695054367	37.0	35.0	37.0	33.0	37.0
9	36.94145360029137	39.0	37.0	39.0	33.0	39.0
10	36.793103612128775	39.0	37.0	39.0	32.0	39.0
11	36.83117534710721	39.0	37.0	39.0	32.0	39.0
12	36.715599748549394	39.0	35.0	39.0	32.0	39.0
13	36.74964424882206	39.0	35.0	39.0	32.0	39.0
14	37.73316411568405	39.0	37.0	41.0	32.0	41.0
15	37.73443646246228	39.0	37.0	41.0	32.0	41.0
16	37.6053746029856	39.0	36.0	41.0	32.0	41.0
17	37.64364255664557	39.0	37.0	41.0	32.0	41.0
18	37.634484566785765	39.0	37.0	41.0	32.0	41.0
19	37.67603457138377	39.0	37.0	41.0	32.0	41.0
20	37.597942610787385	39.0	37.0	41.0	32.0	41.0
21	37.514807103339884	39.0	36.0	41.0	32.0	41.0
22	37.41287932467232	39.0	36.0	41.0	32.0	41.0
23	37.219774426780106	39.0	36.0	41.0	31.0	41.0
24	37.07456170141163	39.0	36.0	41.0	31.0	41.0
25	36.91804152512787	39.0	36.0	40.0	30.0	41.0
26	37.084463756984	39.0	36.0	41.0	31.0	41.0
27	37.022456137997075	39.0	36.0	41.0	31.0	41.0
28	36.93880967933898	39.0	35.0	41.0	31.0	41.0
29	36.85462879031183	39.0	35.0	40.0	30.0	41.0
30	36.62351420950285	39.0	35.0	40.0	30.0	41.0
31	36.40532350786454	38.0	35.0	40.0	30.0	41.0
32	36.26095463463215	38.0	35.0	40.0	30.0	41.0
33	36.083555896189694	38.0	35.0	40.0	30.0	41.0
34	35.95376143106589	38.0	35.0	40.0	29.0	41.0
35	35.74427534791781	38.0	35.0	40.0	28.0	41.0
36	35.63324520263492	38.0	35.0	40.0	27.0	41.0
37	35.45507332904559	38.0	34.0	40.0	27.0	41.0
38	35.349674827630835	38.0	34.0	40.0	26.0	41.0
39	35.23873776044047	38.0	34.0	40.0	25.0	41.0
40	35.03517177939318	38.0	34.0	40.0	24.0	41.0
41	34.870253052220725	38.0	34.0	40.0	24.0	41.0
42	34.63742309527423	38.0	33.0	40.0	23.0	41.0
43	34.30082783599584	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	8.0
15	15.0
16	44.0
17	104.0
18	231.0
19	518.0
20	1202.0
21	2359.0
22	4477.0
23	8075.0
24	13308.0
25	20378.0
26	30262.0
27	42727.0
28	59049.0
29	77140.0
30	97589.0
31	122072.0
32	150976.0
33	186867.0
34	228935.0
35	281019.0
36	361363.0
37	497196.0
38	658607.0
39	733111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16470629669103	22.079521114611556	14.714807885978498	23.040964702718913
2	18.761273140597392	23.457104835281125	35.54895530322914	22.23266672089234
3	19.200724723358213	24.651579071237165	33.46761175869798	22.68008444670664
4	13.56620186318303	16.621902532981927	36.30003681196632	33.51185879186872
5	11.94823628195227	38.93613137819026	35.383371333233264	13.732261006624197
6	31.72143320137487	37.797056775679835	13.10204126126685	17.379468761678435
7	25.14000147024254	34.031279270334835	21.403628815747584	19.425090443675035
8	26.574102879523124	35.14002662648376	18.86117136962596	19.424699124367145
9	24.08626242823134	14.768502485296874	22.40180051603836	38.74343457043342
10	16.3466932540185	27.97452287694601	33.594119700663086	22.0846641683724
11	32.48897807242258	22.59964037755605	23.89669617493762	21.01468537508375
12	20.990507432131956	27.761281805524924	30.751352637114792	20.49685812522833
13	31.393619426879333	21.5065178423436	26.867508506303455	20.23235422447361
14	20.195458404015383	23.47745343929142	28.024527894218565	28.30256026247463
15	21.919834885613174	29.802319461343384	26.446029660326452	21.831815992716987
16	21.372854347319926	28.854683854528723	27.13377334394368	22.638688454207674
17	21.24782782844897	27.125583589857115	27.068982047108697	24.55760653458522
18	22.80539047936895	25.617942102072288	30.27260141529013	21.304066003268634
19	22.583093161108117	24.61320182768478	31.347499651306542	21.456205359900558
20	23.51367842665041	24.153904765738094	31.517360182310078	20.81505662530142
21	22.275264622694117	25.12513133654271	32.199625395616835	20.39997864514634
22	20.443918213146596	25.488387459781457	31.644678714270103	22.423015612801844
23	21.926738876259524	25.656487053899486	32.84728680155443	19.56948726828656
24	21.83939081646258	25.600724052525116	29.943641634250056	22.61624349676225
25	21.492961423463115	26.17339190824345	31.22241722967732	21.111229438616117
26	21.36119862222062	26.930622720390335	31.010685532729525	20.697493124659516
27	20.077699243719536	27.079296106009522	30.46043468307748	22.382569967193465
28	20.663336539356518	26.332407304142592	30.958584162021847	22.045671994479044
29	21.08987458495696	26.070139513718676	30.02514785580778	22.814838045516588
30	21.241538768143588	26.30786599326204	31.86585797736553	20.584737261228838
31	22.254384942480254	25.90969859748365	31.625615873700013	20.21030058633608
32	21.552386055504172	26.02670307054285	30.482069050527986	21.93884182342499
33	21.147845745282996	25.9264973763438	31.392864739642683	21.53279213873052
34	20.057378591088373	26.581509994993908	32.266596900024346	21.094514513893373
35	19.710222462231396	28.70257244927593	30.668979922803878	20.918225165688785
36	22.325073980312727	26.045262786288507	30.472761241276025	21.15690199212274
37	21.196145728428313	27.027641957367997	30.49160047081303	21.284611843390657
38	21.064438829944088	25.32828196009605	31.950382947869837	21.65689626209002
39	20.525620094358267	25.153194521194273	32.69154171701409	21.629643667433374
40	19.128330651398507	25.895722907916134	32.16574832410519	22.81019811658017
41	20.927672731836424	24.808414259563776	32.3370343754446	21.9268786331552
42	19.498015591838307	27.32244515310508	31.37366214217693	21.80587711287969
43	20.01232096792272	27.271881108249822	30.383652244593573	22.332145679233886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	184.0
1	411.5
2	639.0
3	1139.0
4	1639.0
5	1639.0
6	3070.0
7	4501.0
8	5156.5
9	5812.0
10	9809.5
11	13807.0
12	13807.0
13	27478.0
14	41149.0
15	64808.5
16	88468.0
17	83064.5
18	77661.0
19	77661.0
20	84880.5
21	92100.0
22	73593.0
23	55086.0
24	61824.5
25	68563.0
26	68563.0
27	75385.5
28	82208.0
29	87260.5
30	92313.0
31	100855.5
32	109398.0
33	109398.0
34	123797.0
35	138196.0
36	152015.5
37	165835.0
38	175375.0
39	184915.0
40	184915.0
41	191269.5
42	197624.0
43	208146.0
44	218668.0
45	234986.0
46	251304.0
47	251304.0
48	333648.0
49	415992.0
50	375785.0
51	335578.0
52	299445.0
53	263312.0
54	263312.0
55	228479.0
56	193646.0
57	161853.5
58	130061.0
59	116348.0
60	102635.0
61	102635.0
62	91054.0
63	79473.0
64	68838.0
65	58203.0
66	48920.0
67	39637.0
68	39637.0
69	33232.5
70	26828.0
71	22096.5
72	17365.0
73	14077.0
74	10789.0
75	10789.0
76	8678.5
77	6568.0
78	5027.0
79	3486.0
80	2699.0
81	1912.0
82	1912.0
83	1435.0
84	958.0
85	739.5
86	521.0
87	418.0
88	315.0
89	315.0
90	244.0
91	173.0
92	122.5
93	72.0
94	50.5
95	29.0
96	29.0
97	19.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3577641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.80363546334606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25107649430377	28.036044341192945
2	13.83231872321835	10.734885066971625
3	5.4513501928088575	6.345966169943885
4	2.6917418311403223	4.177974751080347
5	1.5306533423220474	2.9697457158108485
6	0.9291928567443141	2.16336365329509
7	0.6160016984252564	1.673217374534698
8	0.42983706289735585	1.3343392557843476
9	0.30433570513635166	1.0628398584532228
>10	1.650905978626744	11.985731036701932
>50	0.17216970438342988	4.670853109406422
>100	0.1207083331791681	9.231271236892324
>500	0.011550521210008134	2.977519361065183
>1k	0.006858121967959182	5.338900740175158
>5k	5.775260604597206E-4	1.2933435149661685
>10k+	7.219075755746507E-4	6.004004813725987
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	44684	1.2489794252693325	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44297	1.238162241544079	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35274	0.9859569476087735	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17562	0.4908821203692601	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	13066	0.3652127197782002	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	13035	0.3643462270250145	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12765	0.3567993546585585	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11586	0.32384467865836736	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11425	0.31934450661762875	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10638	0.2973467712383663	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7047	0.1969733687645015	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6333	0.17701608406209568	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6098	0.17044750996536545	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5796	0.16200619346658873	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5376	0.1502666142298794	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5368	0.15004300319679922	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5120	0.14311106117131372	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5032	0.14065133980743177	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4916	0.1374089798277692	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4742	0.13254543985827533	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4384	0.12253884612793739	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4119	0.11513173065715648	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4114	0.11499197376148138	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3869	0.10814388587340093	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3857	0.10780846932378067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.7951379135022213E-5	0.0	0.0	0.0	0.0
6	2.7951379135022213E-5	0.0	0.0	2.7951379135022213E-5	0.0
7	2.7951379135022213E-5	0.0	0.0	5.5902758270044425E-5	0.0
8	5.5902758270044425E-5	0.0	0.0	5.5902758270044425E-5	0.0
9	5.5902758270044425E-5	0.0	0.0	1.3975689567511106E-4	0.0
10	5.5902758270044425E-5	0.0	0.0	3.6336792875528873E-4	0.0
11	5.5902758270044425E-5	0.0	0.0	7.267358575105775E-4	0.0
12	5.5902758270044425E-5	0.0	0.0	0.0011460065445359107	0.0
13	5.5902758270044425E-5	0.0	0.0	0.0014814230941561772	0.0
14	8.385413740506664E-5	0.0	0.0	0.002012499297721599	0.0
15	8.385413740506664E-5	0.0	0.0	0.002543575501287021	0.0
16	8.385413740506664E-5	0.0	0.0	0.003074651704852443	0.0
17	8.385413740506664E-5	0.0	0.0	0.004695831694683732	0.0
18	8.385413740506664E-5	0.0	0.0	0.005674129964409509	0.0
19	8.385413740506664E-5	0.0	0.0	0.007407115470780886	0.0
20	1.1180551654008885E-4	0.0	0.0	0.012466315094219907	0.0
21	1.1180551654008885E-4	0.0	0.0	0.020152944356351014	0.0
22	1.1180551654008885E-4	0.0	0.0	0.0325913080714359	0.0
23	1.1180551654008885E-4	0.0	0.0	0.03818158389844034	0.0
24	1.1180551654008885E-4	0.0	0.0	0.04729373349645758	0.0
25	1.1180551654008885E-4	0.0	0.0	0.0526044955321118	0.0
26	1.1180551654008885E-4	0.0	0.0	0.06163279099272398	0.0
27	1.1180551654008885E-4	0.0	0.0	0.1162777372016924	0.0
28	1.1180551654008885E-4	0.0	0.0	0.2330585992278152	0.0
29	1.1180551654008885E-4	0.0	0.0	0.3730391059360064	0.0
30	1.3975689567511106E-4	0.0	0.0	0.6150700978661637	0.0
31	1.3975689567511106E-4	0.0	0.0	0.9308368279545097	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11025	0.0	30.120182	1
GCCGTAT	60	3.7291193E-5	21.583334	30
TGCGACG	160	0.0	20.8125	22
GTATTGG	1310	0.0	20.335876	1
GTCTAAC	215	0.0	18.069767	1
GTATCAA	18650	0.0	17.80563	2
ATTGGAC	1530	0.0	17.29085	3
TTAACGG	400	0.0	16.65	35
GGACCCT	2120	0.0	16.40566	6
CTAATAC	840	0.0	16.297619	3
GTATGCG	80	3.385064E-4	16.1875	33
TTGGACC	2335	0.0	16.16274	4
CAATGCG	210	0.0	15.857142	19
TCTAACG	155	7.223207E-9	15.516129	2
TGGACCC	2415	0.0	14.861284	5
CCATGCG	125	2.9627936E-6	14.799999	9
TAATACT	905	0.0	14.718232	4
GACCCTC	2270	0.0	14.588105	7
CTAGTAC	460	0.0	14.47826	3
GTATTAG	735	0.0	14.346938	1
>>END_MODULE