FastQCFastQC Report
Wed 25 May 2016
SRR2088083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3228168
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT573181.7755581493899946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT559011.7316632839430908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT445691.3806282696563499No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210630.6524753358561264No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG178090.5516751296710704No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA168330.5214412632799781No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG164790.5104752912487827No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG148760.4608186438871831No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA142870.4425730011573127No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA138990.43055380017396866No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA92050.2851462501332025No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA77440.23988838251292993No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC75890.23508689758401669No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA75230.2330423943239633No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA69340.214796751594093No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68120.21101751829520643No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA64010.19828583890305584No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG60880.1885899370788633No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57970.17957553634135523No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG57110.17691148663886144No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG53710.1663791971173743No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53380.16535694548734764No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC50190.15547517973042294No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT50160.15538224776405687No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT49620.15370947236946778No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC47510.14717325740172135No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.1463678470265488No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT39920.12366146991110748No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT39790.12325876472352121No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG38880.12043982841041731No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA38240.11845727979460798No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA37680.11672254975577478No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG37170.11514270632755172No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG35920.11127054106229912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC34810.10783205830675478No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC34640.10730544383068044No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA34280.10619026023428767No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT34100.1056326684360913No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33030.10231809496903507No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC32530.10076922886293402No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC32460.10055238760807987No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA32310.10008772777624955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137100.030.4420131
ATTGGAC13850.021.9061373
GTATTGG14100.021.6489351
TTGGACC23050.018.620394
TGCGACG1800.018.522
GTATCAA234250.017.8326572
TTTTTAC27150.017.648251
TATTGGA17350.017.593662
TGGACCC24700.017.0769235
GGACCCT24750.016.9676766
CGACGAG2100.016.73809424
TATACTG3200.015.6093755
GACCCTC26450.015.317587
CTAATAC3050.015.1639353
CAATGCG2600.014.94230719
CGCCCTT1752.242814E-914.810
GATACCT13950.014.58781436
ACGGGTA1651.6481863E-814.5757575
GTATTAG3850.014.4155841
TTAGACG1551.2122109E-714.32258115
Produced by FastQC (version 0.11.5)