##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3228168
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50747637669415	31.0	31.0	34.0	30.0	34.0
2	31.69774125758015	31.0	31.0	34.0	30.0	34.0
3	31.74541783451171	31.0	31.0	34.0	30.0	34.0
4	35.45095050815199	37.0	35.0	37.0	33.0	37.0
5	35.36632696935228	37.0	35.0	37.0	33.0	37.0
6	35.419065240718574	37.0	35.0	37.0	33.0	37.0
7	35.24804347233477	37.0	35.0	37.0	32.0	37.0
8	35.320255947026304	37.0	35.0	37.0	32.0	37.0
9	36.815815967446554	39.0	37.0	39.0	32.0	39.0
10	36.69075896917385	39.0	35.0	39.0	32.0	39.0
11	36.73236213233017	39.0	35.0	39.0	32.0	39.0
12	36.599792823669645	39.0	35.0	39.0	32.0	39.0
13	36.640817020675506	39.0	35.0	39.0	32.0	39.0
14	37.542636256849086	39.0	36.0	41.0	32.0	41.0
15	37.54720819982108	39.0	36.0	41.0	32.0	41.0
16	37.407397632341315	39.0	36.0	41.0	32.0	41.0
17	37.506263924306296	39.0	36.0	41.0	32.0	41.0
18	37.50607744082712	39.0	36.0	41.0	32.0	41.0
19	37.568217019684226	39.0	37.0	41.0	32.0	41.0
20	37.4997605453	39.0	36.0	41.0	32.0	41.0
21	37.42169304695419	39.0	36.0	41.0	32.0	41.0
22	37.31280961833461	39.0	36.0	41.0	31.0	41.0
23	37.09772570696445	39.0	36.0	41.0	31.0	41.0
24	36.93927484567098	39.0	36.0	40.0	31.0	41.0
25	36.77834517906131	39.0	35.0	40.0	30.0	41.0
26	36.91254792191732	39.0	36.0	40.0	31.0	41.0
27	36.84266772980836	39.0	35.0	40.0	31.0	41.0
28	36.73372234654454	39.0	35.0	40.0	30.0	41.0
29	36.614897675709564	39.0	35.0	40.0	30.0	41.0
30	36.344192433603205	38.0	35.0	40.0	30.0	41.0
31	36.064502219215356	38.0	35.0	40.0	30.0	41.0
32	35.91128497649441	38.0	35.0	40.0	29.0	41.0
33	35.68939132040216	38.0	34.0	40.0	29.0	41.0
34	35.5287069941837	38.0	34.0	40.0	27.0	41.0
35	35.279005615568956	38.0	34.0	40.0	26.0	41.0
36	35.129494499666684	38.0	34.0	40.0	25.0	41.0
37	34.92079780234486	38.0	33.0	40.0	25.0	41.0
38	34.763169388953735	38.0	33.0	40.0	24.0	41.0
39	34.59972653220031	38.0	33.0	40.0	23.0	41.0
40	34.3331722512583	38.0	33.0	40.0	22.0	41.0
41	34.11352878784499	38.0	33.0	40.0	21.0	41.0
42	33.807303089554196	38.0	33.0	40.0	18.0	41.0
43	33.44087916118368	37.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	5.0
15	22.0
16	53.0
17	96.0
18	242.0
19	587.0
20	1272.0
21	2731.0
22	4908.0
23	8665.0
24	14325.0
25	22123.0
26	32381.0
27	45264.0
28	60976.0
29	78857.0
30	98280.0
31	119934.0
32	144909.0
33	178909.0
34	216943.0
35	260524.0
36	335209.0
37	459704.0
38	562298.0
39	578946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.250941091046066	22.23976571231733	15.70900275326439	21.800290443372216
2	18.89638333568761	24.192607076211647	35.48672188064562	21.424287707455125
3	19.65396472550375	24.280861466937285	34.789267473068314	21.275906334490646
4	13.546475895926111	16.439478986223765	35.67236897212289	34.341676145727234
5	11.407120075535103	39.50999452320944	34.875694201788754	14.207191199466696
6	31.439844518624803	37.3853529308264	12.133228506075271	19.04157404447352
7	24.159771114762304	35.34131433060485	20.36040255649644	20.138511998136405
8	27.36576906778086	34.681063686896096	18.185856498174815	19.76731074714823
9	23.420156571776936	14.773487625179357	23.03318786382865	38.77316793921506
10	16.63318018145276	28.200050307171125	32.604065215936714	22.562704295439396
11	31.720158306506974	22.29530805088211	24.424317445684363	21.560216196926554
12	21.310972663132773	28.196425960482852	30.830024955330703	19.662576421053675
13	32.99053828673105	21.23430379088077	27.248365016938397	18.526792905449778
14	19.739988749036605	24.12609876561567	28.75287159776071	27.381040887587016
15	21.308866205228476	30.000204450326006	27.283555254869015	21.407374089576503
16	20.400951871154167	29.20548125128556	27.875407971332343	22.518158906227928
17	20.888565898676898	26.682316409802713	27.30062375935825	25.128493932162144
18	23.423966782397944	24.49612287836321	31.294282081973428	20.785628257265422
19	22.291157089717757	23.588982977341946	33.10072462151908	21.019135311421213
20	23.816480431006067	22.701296834613316	33.213451096721116	20.2687716376595
21	22.217957677543424	24.082854423933327	34.318845859323304	19.380342039199945
22	19.59647081564528	24.395105830923296	33.66277715410103	22.345646199330393
23	21.623440911377596	24.693107669737138	35.200894129425734	18.482557289459535
24	21.302515854193462	24.47347845589201	31.732859008577	22.491146681337526
25	20.821902701470307	25.707955719776663	32.99976952872341	20.470372050029614
26	20.732223353927058	26.545768373888844	32.779118063248255	19.942890208935843
27	18.661296438103594	26.82224097382788	32.30339932742039	22.213063260648145
28	19.889578237563843	25.8445966876569	32.27833867382366	21.98748640095559
29	20.066458746880585	25.39316417237269	31.325321358739693	23.215055722007033
30	20.608097224184117	25.831493280399286	33.89758525578594	19.662824239630652
31	21.970603760399086	25.37079854580059	33.421990429246556	19.236607264553765
32	21.323363591981582	25.40533825996664	31.903698940079945	21.36759920797183
33	20.527834982565963	25.28861571021087	32.98288688816691	21.200662419056258
34	18.910261175998276	26.59892545865023	33.975431266278584	20.51538209907291
35	18.47809655507396	29.534863117409003	32.01060167872304	19.97643864879399
36	21.94151605492651	25.854416498769584	31.843881731062325	20.360185715241585
37	20.822584202556992	27.446186196009627	31.45570490755128	20.2755246938821
38	20.541465004299653	24.888853368226187	33.46740318347744	21.10227844399672
39	19.905407649168197	24.629480250098506	34.44495453768205	21.020157563051242
40	17.91139742417371	25.45474708875127	33.814937760364394	22.81891772671063
41	20.54651430780554	24.515421750045228	33.624582115924575	21.313481826224656
42	18.8002916824651	27.5028437181708	32.607875426557726	21.08898917280637
43	19.28443624991017	27.963972135279203	30.882624448293893	21.868967166516736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	378.5
2	520.0
3	1061.5
4	1603.0
5	1603.0
6	3112.5
7	4622.0
8	5657.5
9	6693.0
10	11194.5
11	15696.0
12	15696.0
13	31904.5
14	48113.0
15	79630.0
16	111147.0
17	102521.0
18	93895.0
19	93895.0
20	100850.5
21	107806.0
22	82359.5
23	56913.0
24	61457.0
25	66001.0
26	66001.0
27	69242.0
28	72483.0
29	74648.0
30	76813.0
31	82202.0
32	87591.0
33	87591.0
34	95944.5
35	104298.0
36	114725.5
37	125153.0
38	130261.0
39	135369.0
40	135369.0
41	141920.5
42	148472.0
43	157196.0
44	165920.0
45	192080.5
46	218241.0
47	218241.0
48	328459.5
49	438678.0
50	389537.0
51	340396.0
52	298249.5
53	256103.0
54	256103.0
55	215457.5
56	174812.0
57	140077.0
58	105342.0
59	91909.0
60	78476.0
61	78476.0
62	68240.5
63	58005.0
64	50821.5
65	43638.0
66	37130.0
67	30622.0
68	30622.0
69	25642.0
70	20662.0
71	17340.0
72	14018.0
73	11430.0
74	8842.0
75	8842.0
76	6913.5
77	4985.0
78	3900.5
79	2816.0
80	2173.5
81	1531.0
82	1531.0
83	1192.5
84	854.0
85	633.5
86	413.0
87	315.5
88	218.0
89	218.0
90	158.0
91	98.0
92	69.5
93	41.0
94	30.0
95	19.0
96	19.0
97	14.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3228168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.145861735790206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26746693499032	26.121698678279508
2	13.384448118850662	9.675848222276667
3	5.517994752038076	5.9835802609795286
4	2.8495833510530666	4.120025832470955
5	1.569712830840078	2.8369311474220655
6	0.9668601772513356	2.0968796570861032
7	0.6489003504245257	1.6418543642725503
8	0.4495383774517226	1.299916162904115
9	0.31510448837386984	1.0250750942179931
>10	1.7041372822168692	11.503084254533583
>50	0.17836491457614836	4.521328356049833
>100	0.12656259894185012	8.653233477706737
>500	0.011178858826190205	2.7721031641066793
>1k	0.008083174843548334	6.44748812190135
>5k	0.0012038771043582626	2.8144372132781355
>10k+	8.599122173987589E-4	8.486515992514272
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57318	1.7755581493899946	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	55901	1.7316632839430908	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	44569	1.3806282696563499	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21063	0.6524753358561264	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	17809	0.5516751296710704	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	16833	0.5214412632799781	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16479	0.5104752912487827	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	14876	0.4608186438871831	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14287	0.4425730011573127	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13899	0.43055380017396866	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9205	0.2851462501332025	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7744	0.23988838251292993	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7589	0.23508689758401669	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7523	0.2330423943239633	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6934	0.214796751594093	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6812	0.21101751829520643	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	6401	0.19828583890305584	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	6088	0.1885899370788633	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5797	0.17957553634135523	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5711	0.17691148663886144	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5371	0.1663791971173743	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5338	0.16535694548734764	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5019	0.15547517973042294	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5016	0.15538224776405687	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4962	0.15370947236946778	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4751	0.14717325740172135	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4725	0.1463678470265488	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3992	0.12366146991110748	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3979	0.12325876472352121	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3888	0.12043982841041731	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	3824	0.11845727979460798	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3768	0.11672254975577478	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3717	0.11514270632755172	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3592	0.11127054106229912	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3481	0.10783205830675478	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3464	0.10730544383068044	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3428	0.10619026023428767	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3410	0.1056326684360913	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3303	0.10231809496903507	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	3253	0.10076922886293402	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3246	0.10055238760807987	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3231	0.10008772777624955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.097732212202091E-5	0.0	0.0	0.0	0.0
6	3.097732212202091E-5	0.0	0.0	0.0	0.0
7	3.097732212202091E-5	0.0	0.0	3.097732212202091E-5	0.0
8	3.097732212202091E-5	0.0	0.0	6.195464424404182E-5	0.0
9	3.097732212202091E-5	0.0	0.0	1.5488661061010455E-4	0.0
10	3.097732212202091E-5	0.0	0.0	5.266144760743555E-4	0.0
11	3.097732212202091E-5	0.0	0.0	9.293196636606273E-4	0.0
12	3.097732212202091E-5	0.0	0.0	0.0016108207503450873	0.0
13	3.097732212202091E-5	0.0	0.0	0.0018896166494432756	0.0
14	3.097732212202091E-5	0.0	0.0	0.002416231125517631	0.0
15	3.097732212202091E-5	0.0	0.0	0.0032526188228121956	0.0
16	3.097732212202091E-5	0.0	0.0	0.00477050760679122	0.0
17	6.195464424404182E-5	0.0	0.0	0.0067220789004785375	0.0
18	6.195464424404182E-5	0.0	0.0	0.008116058395969478	0.0
19	9.293196636606273E-5	0.0	0.0	0.010408380232999026	0.0
20	9.293196636606273E-5	0.0	0.0	0.018245642729870316	0.0
21	9.293196636606273E-5	0.0	0.0	0.03131807266536314	0.0
22	9.293196636606273E-5	0.0	0.0	0.050152284515551857	0.0
23	9.293196636606273E-5	0.0	0.0	0.061118256546747254	0.0
24	1.2390928848808364E-4	0.0	0.0	0.07493414221316859	0.0
25	1.2390928848808364E-4	0.0	0.0	0.08128449324818288	0.0
26	1.5488661061010455E-4	0.0	0.0	0.09299392101030678	0.0
27	1.8586393273212546E-4	0.0	0.0	0.16417980724671083	0.0
28	1.8586393273212546E-4	0.0	0.0	0.30466196307007565	0.0
29	1.8586393273212546E-4	0.0	0.0	0.477019783357	0.0
30	2.1684125485414636E-4	0.0	0.0	0.7805665628306829	0.0
31	2.478185769761673E-4	0.0	0.0	1.1385404972727566	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13710	0.0	30.442013	1
ATTGGAC	1385	0.0	21.906137	3
GTATTGG	1410	0.0	21.648935	1
TTGGACC	2305	0.0	18.62039	4
TGCGACG	180	0.0	18.5	22
GTATCAA	23425	0.0	17.832657	2
TTTTTAC	2715	0.0	17.64825	1
TATTGGA	1735	0.0	17.59366	2
TGGACCC	2470	0.0	17.076923	5
GGACCCT	2475	0.0	16.967676	6
CGACGAG	210	0.0	16.738094	24
TATACTG	320	0.0	15.609375	5
GACCCTC	2645	0.0	15.31758	7
CTAATAC	305	0.0	15.163935	3
CAATGCG	260	0.0	14.942307	19
CGCCCTT	175	2.242814E-9	14.8	10
GATACCT	1395	0.0	14.587814	36
ACGGGTA	165	1.6481863E-8	14.575757	5
GTATTAG	385	0.0	14.415584	1
TTAGACG	155	1.2122109E-7	14.322581	15
>>END_MODULE