FastQCFastQC Report
Wed 25 May 2016
SRR2088082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191492
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT301761.3769614490949544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT290781.3268585967915922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230971.0539395078786506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110760.50540910028419No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG85590.3905558404958813No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA81260.37079761185530224No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG75350.343829683156498No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69500.31713554053585413No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA66690.3043132258753397No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66640.30408507081020597No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38470.17554250711387492No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.16285708549244077No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA33770.15409593099130636No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC33130.1511755461575949No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32210.1469774929591347No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA31140.14209497456527334No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31110.14195808152619313No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30050.1371211941453585No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27840.1270367402664486No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG27730.12653479912315446No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.1132105433193459No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG24320.11097462368103556No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC24200.11042705152471467No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT23990.1094688002511531No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23590.10764355973008344No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT23200.10586395022204051No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC22490.10262414829714185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.031.5274161
TAAGCCG1254.129106E-1019.2436
GTATTGG6600.019.0606081
GTATCAA116750.018.7297652
ATTGGAC7400.017.253
TTGGACC11950.017.18414
TACTAGG1851.8189894E-1217.02
TCACGTT1504.6766218E-916.03333324
GGACCCT12200.015.4672146
TGGACCC12250.015.4040815
GTACGAT800.006301024513.8756
TTTGGAC7300.013.4315073
GACCCTC13550.013.38007457
TCTATAC2501.0913936E-1113.323
TTTTTAC15650.013.2396161
TATCAAC186700.013.1788961
GCGATTG850.0094082913.0588239
TATTGGA9950.013.0150762
CGTACTT1000.001833402412.9499995
ATCAACG190850.012.9020182
Produced by FastQC (version 0.11.5)