##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2191492
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35419111728448	31.0	31.0	34.0	30.0	34.0
2	31.55511815694513	31.0	31.0	34.0	30.0	34.0
3	31.616166520343217	31.0	31.0	34.0	30.0	34.0
4	35.33956181450811	37.0	35.0	37.0	33.0	37.0
5	35.22286597441378	37.0	35.0	37.0	33.0	37.0
6	35.278100034131995	37.0	35.0	37.0	32.0	37.0
7	35.12229841587375	37.0	35.0	37.0	32.0	37.0
8	35.176756748370515	37.0	35.0	37.0	32.0	37.0
9	36.66676036234675	39.0	35.0	39.0	32.0	39.0
10	36.487770888508834	38.0	35.0	39.0	32.0	39.0
11	36.55709899922062	38.0	35.0	39.0	32.0	39.0
12	36.42094700779195	38.0	35.0	39.0	32.0	39.0
13	36.46965446371696	38.0	35.0	39.0	32.0	39.0
14	37.38034727026154	39.0	36.0	41.0	32.0	41.0
15	37.38942190982217	39.0	36.0	41.0	32.0	41.0
16	37.24523977272105	39.0	36.0	40.0	32.0	41.0
17	37.297992874261006	39.0	36.0	40.0	32.0	41.0
18	37.29351190878178	39.0	36.0	40.0	32.0	41.0
19	37.340792026619305	39.0	36.0	40.0	31.0	41.0
20	37.267451580932075	39.0	36.0	40.0	31.0	41.0
21	37.188559666200014	39.0	36.0	40.0	31.0	41.0
22	37.072577495149424	39.0	36.0	40.0	31.0	41.0
23	36.874606888822775	39.0	36.0	40.0	31.0	41.0
24	36.728316598919825	39.0	35.0	40.0	30.0	41.0
25	36.578756390623376	38.0	35.0	40.0	30.0	41.0
26	36.71568730344441	39.0	35.0	40.0	30.0	41.0
27	36.62640338180564	39.0	35.0	40.0	30.0	41.0
28	36.541423377315546	39.0	35.0	40.0	30.0	41.0
29	36.450279079275674	39.0	35.0	40.0	30.0	41.0
30	36.20541393717157	38.0	35.0	40.0	30.0	41.0
31	35.96962024045719	38.0	35.0	40.0	29.0	41.0
32	35.82346638728318	38.0	35.0	40.0	29.0	41.0
33	35.627936583843336	38.0	34.0	40.0	27.0	41.0
34	35.49528768528473	38.0	34.0	40.0	27.0	41.0
35	35.26524988455354	38.0	34.0	40.0	26.0	41.0
36	35.156358772927305	38.0	34.0	40.0	25.0	41.0
37	34.96778815528416	38.0	33.0	40.0	25.0	41.0
38	34.84943317155618	38.0	33.0	40.0	24.0	41.0
39	34.73352537905682	38.0	33.0	40.0	24.0	41.0
40	34.52091543113094	38.0	33.0	40.0	23.0	41.0
41	34.3534194968542	38.0	33.0	40.0	22.0	41.0
42	34.114455357354714	38.0	33.0	40.0	21.0	41.0
43	33.77490495059987	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	6.0
14	7.0
15	12.0
16	38.0
17	78.0
18	195.0
19	467.0
20	1000.0
21	2072.0
22	3640.0
23	6406.0
24	10537.0
25	15631.0
26	22835.0
27	31511.0
28	41980.0
29	54422.0
30	68517.0
31	84289.0
32	102340.0
33	123459.0
34	150464.0
35	181425.0
36	230991.0
37	309894.0
38	400600.0
39	348674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.20548192738098	23.17402938272191	15.427708611302254	22.192780078594858
2	19.034201356883802	24.413869637671503	35.11844898361481	21.433480021829876
3	19.68991901407808	25.2390608772471	33.671854608641056	21.399165500033767
4	13.968839493824298	17.29419956814809	36.25237965732934	32.484581280698265
5	11.751126629711631	39.53042037114441	35.208935282446845	13.509517716697117
6	31.36707777167336	38.49728860520595	13.107234705853363	17.028398917267324
7	24.557607328705743	34.47610121323738	21.553763372168365	19.412528085888518
8	26.412234222164628	35.65607357909589	18.88060736703579	19.051084831703697
9	23.871955726965922	14.698707547187032	22.552169937193472	38.877166788653575
10	16.074710745008424	28.59430926510341	33.68718662901804	21.643793360870127
11	32.43566483473359	22.85328899215694	23.830203350046453	20.88084282306301
12	20.545683032381593	28.18185053835469	31.241364330784688	20.03110209847903
13	31.464454353472426	21.686823406154346	27.083968364931287	19.764753875441937
14	19.908126518371958	23.62002690404528	28.524493815172498	27.947352762410265
15	21.739207809109047	30.20882576801558	26.639796084128985	21.41217033874639
16	20.81823707319032	29.215758031514603	27.47607565987008	22.489929235425
17	20.934002953239165	27.311302071830518	27.76656268879832	23.988132286132004
18	22.571836903808	25.60908276188095	30.798241563282	21.02083877102905
19	22.13017432872217	24.660459632068015	32.16055545719537	21.048810582014447
20	23.23658950158157	24.319641595771284	32.09717398010123	20.34659492254592
21	21.987668675039654	25.232216225293087	32.78323626095829	19.99687883870897
22	20.127474797991503	25.548986717724727	32.43885900564547	21.884679478638297
23	21.531084758694078	25.792930113365685	33.45104613660465	19.224938991335584
24	21.477012008257386	25.620764301215793	30.776612463107327	22.12561122741949
25	21.234756960098416	26.258092660160294	31.685445349560936	20.82170503018035
26	21.050179512405247	27.058734414727503	31.579216351234685	20.31186972163257
27	19.671940394945544	27.08743632192132	31.329112768835117	21.911510514298023
28	20.424258906717434	26.541917561186622	31.429044687363678	21.604778844732266
29	20.683716846787487	26.077530741613476	30.76931150102305	22.46944091057599
30	21.108404685027367	26.309518811841432	32.356768813210365	20.22530768992084
31	21.8874173394199	26.17408596517806	32.089416707886684	19.849079987515356
32	21.20304340604483	25.999410447311693	31.23666433644294	21.560881810200538
33	20.751296377080088	26.00662014736992	32.00956243508988	21.232521040460107
34	19.821792641725363	26.702949406157995	32.77826248053837	20.696995471578266
35	19.547824039512808	28.79426436418659	31.19792360638323	20.459987989917373
36	21.958966767845833	26.21091019269064	31.06500046543633	20.765122574027192
37	21.000715494284258	27.189923577179382	31.01453256502876	20.7948283635076
38	20.867974877389468	25.419622795793916	32.39477944706164	21.31762287975498
39	20.272444526377463	25.27196083763938	33.198113431397424	21.257481204585734
40	18.853228759219746	25.991698806110175	32.676140273384526	22.478932161285552
41	20.705984781144533	25.050148483316388	32.61960344824439	21.624263287294685
42	19.36379416397596	27.375961217289408	31.759823900794526	21.500420717940106
43	19.924873100152773	27.41346990999739	30.565340872793513	22.096316117056325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	247.0
2	359.0
3	652.0
4	945.0
5	945.0
6	1841.5
7	2738.0
8	3191.0
9	3644.0
10	6036.0
11	8428.0
12	8428.0
13	17093.0
14	25758.0
15	42424.0
16	59090.0
17	55196.0
18	51302.0
19	51302.0
20	56302.5
21	61303.0
22	49631.0
23	37959.0
24	42020.0
25	46081.0
26	46081.0
27	50520.0
28	54959.0
29	58162.5
30	61366.0
31	67542.0
32	73718.0
33	73718.0
34	82083.5
35	90449.0
36	98321.0
37	106193.0
38	110839.0
39	115485.0
40	115485.0
41	119377.5
42	123270.0
43	127540.0
44	131810.0
45	141028.5
46	150247.0
47	150247.0
48	198712.0
49	247177.0
50	222460.0
51	197743.0
52	176248.0
53	154753.0
54	154753.0
55	133415.5
56	112078.0
57	94067.5
58	76057.0
59	67434.0
60	58811.0
61	58811.0
62	51919.5
63	45028.0
64	38647.5
65	32267.0
66	27127.5
67	21988.0
68	21988.0
69	18714.0
70	15440.0
71	12865.5
72	10291.0
73	8259.5
74	6228.0
75	6228.0
76	5012.5
77	3797.0
78	2944.5
79	2092.0
80	1623.0
81	1154.0
82	1154.0
83	886.0
84	618.0
85	461.5
86	305.0
87	255.5
88	206.0
89	206.0
90	175.5
91	145.0
92	95.0
93	45.0
94	35.0
95	25.0
96	25.0
97	14.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2191492.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.91643406169309
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94528617138909	35.330531588466926
2	12.616533161608166	11.586124260042912
3	4.443581374495808	6.121002335394129
4	1.9364848482588677	3.556659153861842
5	1.0463733745443906	2.40228670280894
6	0.6302812698922514	1.7364161021596651
7	0.3998072323612536	1.2850405695472455
8	0.28179441672486844	1.035119580360055
9	0.21133910915125068	0.8733544443000327
>10	1.2578794210525597	11.095123804558058
>50	0.1296840425348407	4.154095752101424
>100	0.08774175838863288	7.751645813695379
>500	0.0066565858125343215	2.122191599724197
>1k	0.00556371351495406	4.6581923138314165
>5k	5.961121623165064E-4	2.0301782623070928
>10k+	3.9740810821100426E-4	4.262037716840769
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30176	1.3769614490949544	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29078	1.3268585967915922	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23097	1.0539395078786506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11076	0.50540910028419	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8559	0.3905558404958813	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8126	0.37079761185530224	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7535	0.343829683156498	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6950	0.31713554053585413	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6669	0.3043132258753397	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6664	0.30408507081020597	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3847	0.17554250711387492	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3569	0.16285708549244077	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3377	0.15409593099130636	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3313	0.1511755461575949	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3221	0.1469774929591347	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3114	0.14209497456527334	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3111	0.14195808152619313	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3005	0.1371211941453585	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2784	0.1270367402664486	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2773	0.12653479912315446	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2481	0.1132105433193459	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2432	0.11097462368103556	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2420	0.11042705152471467	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2399	0.1094688002511531	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2359	0.10764355973008344	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2320	0.10586395022204051	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2249	0.10262414829714185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.56310130267416E-5	0.0
5	0.0	0.0	0.0	4.56310130267416E-5	0.0
6	0.0	0.0	0.0	4.56310130267416E-5	0.0
7	0.0	0.0	0.0	1.825240521069664E-4	0.0
8	0.0	0.0	0.0	1.825240521069664E-4	0.0
9	0.0	0.0	0.0	1.825240521069664E-4	0.0
10	0.0	0.0	0.0	4.1067911724067437E-4	0.0
11	0.0	0.0	0.0	7.300962084278656E-4	0.0
12	0.0	0.0	0.0	0.00114077532566854	0.0
13	4.56310130267416E-5	0.0	0.0	0.0014145614038289895	0.0
14	9.12620260534832E-5	0.0	0.0	0.0018708715340964056	0.0
15	9.12620260534832E-5	0.0	0.0	0.002372812677390563	0.0
16	1.368930390802248E-4	0.0	0.0	0.0032398019248986534	0.0
17	1.368930390802248E-4	0.0	0.0	0.004517470289647418	0.0
18	1.368930390802248E-4	0.0	0.0	0.005247566498075284	0.0
19	1.368930390802248E-4	0.0	0.0	0.006935913980064723	0.0
20	1.368930390802248E-4	0.0	0.0	0.011362122243658658	0.0
21	1.368930390802248E-4	0.0	0.0	0.01907376344517799	0.0
22	1.368930390802248E-4	0.0	0.0	0.029705789480408782	0.0
23	1.368930390802248E-4	0.0	0.0	0.03499898699151081	0.0
24	1.368930390802248E-4	0.0	0.0	0.04407955858383238	0.0
25	1.368930390802248E-4	0.0	0.0	0.04896207697769374	0.0
26	1.368930390802248E-4	0.0	0.0	0.05653682514013284	0.0
27	1.368930390802248E-4	0.0	0.0	0.10486006793545219	0.0
28	1.368930390802248E-4	0.0	0.0	0.2083055744670754	0.0
29	1.368930390802248E-4	0.0	0.0	0.3295015450661011	0.0
30	1.368930390802248E-4	0.0	0.0	0.5365750821814544	0.0
31	1.368930390802248E-4	0.0	0.0	0.8125514489671877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6930	0.0	31.527416	1
TAAGCCG	125	4.129106E-10	19.24	36
GTATTGG	660	0.0	19.060608	1
GTATCAA	11675	0.0	18.729765	2
ATTGGAC	740	0.0	17.25	3
TTGGACC	1195	0.0	17.1841	4
TACTAGG	185	1.8189894E-12	17.0	2
TCACGTT	150	4.6766218E-9	16.033333	24
GGACCCT	1220	0.0	15.467214	6
TGGACCC	1225	0.0	15.404081	5
GTACGAT	80	0.0063010245	13.875	6
TTTGGAC	730	0.0	13.431507	3
GACCCTC	1355	0.0	13.3800745	7
TCTATAC	250	1.0913936E-11	13.32	3
TTTTTAC	1565	0.0	13.239616	1
TATCAAC	18670	0.0	13.178896	1
GCGATTG	85	0.00940829	13.058823	9
TATTGGA	995	0.0	13.015076	2
CGTACTT	100	0.0018334024	12.949999	5
ATCAACG	19085	0.0	12.902018	2
>>END_MODULE