FastQCFastQC Report
Wed 25 May 2016
SRR2088081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570235
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126942.2260997658859942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110651.9404280691293943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59821.049041184774698No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.7347847817128026No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36480.6397362490902874No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA28820.5054056660850351No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27690.48558927459731516No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.25217673415346303No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.24761721044832394No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG13580.2381474304453427No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12720.22306592895911334No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11690.20500320043490844No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA10620.18623900672529747No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC9780.1715082378317711No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9680.16975457486825607No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8530.1495874507878331No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.1457293922681No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG8230.14432646189728796No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC8150.1429235315264759No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8070.1415206011556639No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG7420.1301217918928161No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7040.12345787263145896No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.12310714003875595No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA6700.11749541855550782No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA6610.11591712188834427No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA6300.11048076670144764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6160.10802563855252659No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC5950.104342946329145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.030.4354841
CTCGTAT250.00549404929.69
AATCTCG250.00549404929.66
AATCGTA250.00549404929.618
GCCGTCA451.32207E-424.66666613
CGTTTAT701.9193976E-723.7857151
GACGGAT400.001930000723.12522
TAGGACC802.719753E-823.1254
ATCTCGT502.6997883E-422.27
GTATCAA39300.021.5597952
TAATACT705.0944855E-621.1428594
GGTACAC450.003823479420.5555553
ACCGCAC555.1387266E-420.1818188
GCGCAGT555.1387266E-420.18181811
CGCAGTT555.1387266E-420.18181812
GTACACA656.8943344E-519.9230774
TAAGCCG656.8943344E-519.92307710
TCGGGAT902.1483484E-618.523
CGAATTA701.21783036E-418.515
AACTGCG801.6143438E-518.57
Produced by FastQC (version 0.11.5)