FastQCFastQC Report
Wed 25 May 2016
SRR2088080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685095
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140900.8361546381658008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136900.8124171040801854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101000.5993727356617876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52230.30995285132292244No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36350.2157148410030295No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG35590.2112047095267626No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA32410.19233336992869837No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31560.18728914393550514No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA30640.18182951109581358No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG27050.1605250742539738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.030.0431771
GTATTGG4900.019.2551041
GTATCAA57000.018.9543862
TCTAACG500.007036210518.52
GGGTAAG2150.016.3488391
TATACCG1403.475907E-815.8571425
TAATACT6850.015.1240874
GGACCCT7850.015.0828036
CGCCTAC750.004105461814.87
CTAGTAC1252.9605435E-614.7999993
TTGGACC8700.014.4597694
TCTAATA5850.014.230772
CTCTAAT4450.014.1348321
GTCTAAC1051.6563539E-414.0952381
GACCCTC8400.014.0952387
TACCGCA2009.840733E-1013.8750017
TGGACCC8400.013.8755
TATTGCG1203.3021537E-513.8749999
CGAACTA3900.013.75641124
TAGTACC1753.5683115E-813.7428574
Produced by FastQC (version 0.11.5)