##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1685095
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298830629727107	31.0	31.0	33.0	30.0	34.0
2	31.52344645257389	31.0	31.0	34.0	30.0	34.0
3	31.58053937611826	31.0	31.0	34.0	30.0	34.0
4	35.32491165186533	37.0	35.0	37.0	33.0	37.0
5	35.1782350549969	37.0	35.0	37.0	32.0	37.0
6	35.23583062082553	37.0	35.0	37.0	32.0	37.0
7	35.11299540975435	37.0	35.0	37.0	32.0	37.0
8	35.14242995202051	37.0	35.0	37.0	32.0	37.0
9	36.65960494808898	39.0	35.0	39.0	32.0	39.0
10	36.42427222204089	38.0	35.0	39.0	32.0	39.0
11	36.510152246609245	38.0	35.0	39.0	32.0	39.0
12	36.353158130550504	38.0	35.0	39.0	32.0	39.0
13	36.39551954044134	38.0	35.0	39.0	32.0	39.0
14	37.35356285550666	39.0	36.0	40.0	32.0	41.0
15	37.35593008109335	39.0	36.0	40.0	32.0	41.0
16	37.21592017067287	39.0	36.0	40.0	32.0	41.0
17	37.19093166854094	39.0	36.0	40.0	31.0	41.0
18	37.15852103293879	39.0	36.0	40.0	31.0	41.0
19	37.19173340375468	39.0	36.0	40.0	31.0	41.0
20	37.095070604327944	39.0	36.0	40.0	31.0	41.0
21	36.99311908230693	39.0	36.0	40.0	31.0	41.0
22	36.8613716140633	39.0	36.0	40.0	31.0	41.0
23	36.649702242306816	39.0	35.0	40.0	30.0	41.0
24	36.51258712416807	38.0	35.0	40.0	30.0	41.0
25	36.33782665072296	38.0	35.0	40.0	30.0	41.0
26	36.52077894718102	38.0	35.0	40.0	30.0	41.0
27	36.44694394084606	38.0	35.0	40.0	30.0	41.0
28	36.37413558286031	38.0	35.0	40.0	30.0	41.0
29	36.2898584352811	38.0	35.0	40.0	30.0	41.0
30	36.062775689204464	38.0	35.0	40.0	29.0	41.0
31	35.89593108993855	38.0	35.0	40.0	29.0	41.0
32	35.74605111284527	38.0	34.0	40.0	27.0	41.0
33	35.58764461350844	38.0	34.0	40.0	27.0	41.0
34	35.45501766962693	38.0	34.0	40.0	27.0	41.0
35	35.255275815310114	38.0	34.0	40.0	26.0	41.0
36	35.18650046436551	38.0	34.0	40.0	26.0	41.0
37	35.019273097362465	38.0	33.0	40.0	25.0	41.0
38	34.94985030517567	38.0	33.0	40.0	25.0	41.0
39	34.885821274171484	38.0	33.0	40.0	24.0	41.0
40	34.72051724086773	38.0	33.0	40.0	24.0	41.0
41	34.59360451487898	38.0	33.0	40.0	24.0	41.0
42	34.416002658603816	38.0	33.0	40.0	23.0	41.0
43	34.09164231096763	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	7.0
15	10.0
16	41.0
17	75.0
18	193.0
19	418.0
20	886.0
21	1585.0
22	3064.0
23	5229.0
24	8005.0
25	12251.0
26	17550.0
27	24209.0
28	31812.0
29	41566.0
30	52177.0
31	64754.0
32	78643.0
33	96293.0
34	116966.0
35	144034.0
36	183775.0
37	241842.0
38	325102.0
39	234602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.07221848026373	23.15003011699637	14.564638788911012	23.213112613828894
2	19.699542162311324	23.97674908536314	34.16038858343298	22.163320168892557
3	19.82819959705536	25.59244434290055	32.19029194199734	22.389064118046758
4	14.61223254475267	17.74534966871304	36.65128672270703	30.99113106382726
5	12.236402101958642	39.20123197801904	35.69009462374525	12.872271296277066
6	31.755301629878435	39.11856601556589	13.802189194081047	15.323943160474633
7	25.904830291467245	33.17195766410796	22.243137627255436	18.680074417169358
8	26.23507873443337	35.95791335206621	19.14538942908263	18.66161848441779
9	24.737240333631043	14.542444194540959	21.364196083900314	39.356119387927684
10	15.94978324664188	28.17734311715363	34.32305003575466	21.549823600449827
11	33.80141772422326	22.836516635560606	23.110091715897322	20.251973924318804
12	20.503947848637612	27.563312454193976	30.84876520314878	21.083974494019625
13	30.268619870096348	21.802272275450346	26.299585483311027	21.62952237114228
14	20.49403742815687	22.582703052350166	27.34160388583433	29.581655633658638
15	22.669285707927447	29.918313210827876	25.225758785113005	22.186642296131673
16	22.17637581263964	28.70882650533056	26.165053008880808	22.94974467314899
17	21.81853248629899	27.562719015841836	26.984769404692315	23.633979093166854
18	22.47345105172112	26.60716458122539	29.19954067871544	21.71984368833805
19	22.7629302798952	25.536957856975423	29.88941276307864	21.81069910005074
20	23.257086395722496	25.532032318652657	29.864488352288742	21.3463929333361
21	22.315715137722204	26.24890584803824	30.12542319572487	21.309955818514684
22	21.575044730415794	26.464917408217342	29.72675131075696	22.233286550609908
23	22.281770463979775	26.581824763588997	30.4954320082844	20.640972764146827
24	22.381290075633718	26.79688682240467	28.156216711817432	22.665606390144173
25	22.30170999261169	26.727751254380316	29.327842050448194	21.642696702559796
26	22.13323284443904	27.33881472557927	29.121384847738558	21.406567582243138
27	21.58234402214712	27.220305086656836	28.6650307549426	22.532320136253446
28	21.59765473163234	26.80519495933464	29.585453639112337	22.011696669920685
29	22.23880552728481	26.590845026541533	28.8370685332281	22.33328091294556
30	22.031220791706104	26.618677285256915	29.850720582519084	21.499381340517893
31	22.548046252585166	26.675291304051107	29.794225251395325	20.982437191968405
32	21.90725151994398	26.375545592384995	29.186307003462712	22.530895884208306
33	21.784706500226992	26.503787620282537	29.73838270245891	21.97312317703156
34	21.33399007177637	26.748462252870013	30.420540088244287	21.497007587109334
35	21.057981894195876	28.12488316682442	29.225058527857477	21.59207641112222
36	22.70536675973758	26.52503271328916	28.962343369364934	21.807257157608326
37	21.851527658678	26.714814298303658	29.346357327034973	22.08730071598337
38	21.744234004611016	25.606983582527988	30.341909506585687	22.306872906275313
39	21.329954690981815	25.836881600147173	30.8183811595192	22.014782549351818
40	20.505550132188393	26.32071188864723	30.45626507704313	22.717472902121244
41	21.50033084188132	25.25810117530466	30.704619027413884	22.536948955400142
42	20.467095326969694	27.097225972422923	29.994866758253984	22.4408119423534
43	21.046943940846067	26.442010687824723	29.557977443408234	22.953067927920976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	138.0
2	179.0
3	296.5
4	414.0
5	414.0
6	895.0
7	1376.0
8	1514.5
9	1653.0
10	2861.5
11	4070.0
12	4070.0
13	8273.5
14	12477.0
15	20278.5
16	28080.0
17	26308.5
18	24537.0
19	24537.0
20	27973.0
21	31409.0
22	26668.0
23	21927.0
24	25746.5
25	29566.0
26	29566.0
27	33261.0
28	36956.0
29	41217.0
30	45478.0
31	52229.0
32	58980.0
33	58980.0
34	67569.5
35	76159.0
36	84331.0
37	92503.0
38	99283.0
39	106063.0
40	106063.0
41	108993.5
42	111924.0
43	116288.5
44	120653.0
45	123164.5
46	125676.0
47	125676.0
48	144539.0
49	163402.0
50	150268.5
51	137135.0
52	125785.0
53	114435.0
54	114435.0
55	101846.0
56	89257.0
57	77561.0
58	65865.0
59	59764.5
60	53664.0
61	53664.0
62	47687.0
63	41710.0
64	36058.5
65	30407.0
66	25515.0
67	20623.0
68	20623.0
69	17622.0
70	14621.0
71	12189.5
72	9758.0
73	7878.5
74	5999.0
75	5999.0
76	4756.5
77	3514.0
78	2803.5
79	2093.0
80	1609.0
81	1125.0
82	1125.0
83	860.5
84	596.0
85	461.0
86	326.0
87	264.5
88	203.0
89	203.0
90	158.0
91	113.0
92	85.0
93	57.0
94	33.0
95	9.0
96	9.0
97	6.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1685095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.095548188652195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64157356351248	40.693298598345415
2	11.523313738340708	11.775800648206909
3	3.7004380647511326	5.672277343698432
4	1.6181794874963566	3.3072707192503445
5	0.8568956282616894	2.1891775933245277
6	0.5096359350006188	1.5624076485295761
7	0.3450854847583459	1.2342632410972127
8	0.2401227573393546	0.9815363135060025
9	0.18896844864964113	0.8689901826701328
>10	1.1646181939749292	11.479494940699954
>50	0.11785163173014086	4.150353504535166
>100	0.08201673567194767	8.121580362926217
>500	0.007572386426050987	2.570010045267724
>1k	0.0032619510758373485	2.827814642975993
>5k	1.1649825270847674E-4	0.31090127310789395
>10k+	3.494947581254302E-4	2.254822941858515
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14090	0.8361546381658008	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13690	0.8124171040801854	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10100	0.5993727356617876	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5223	0.30995285132292244	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3635	0.2157148410030295	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3559	0.2112047095267626	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3241	0.19233336992869837	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3156	0.18728914393550514	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3064	0.18182951109581358	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2705	0.1605250742539738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.934383521403838E-5	0.0
5	0.0	5.934383521403838E-5	0.0	5.934383521403838E-5	0.0
6	0.0	5.934383521403838E-5	0.0	5.934383521403838E-5	0.0
7	0.0	5.934383521403838E-5	0.0	1.1868767042807675E-4	0.0
8	5.934383521403838E-5	5.934383521403838E-5	0.0	1.1868767042807675E-4	0.0
9	5.934383521403838E-5	1.1868767042807675E-4	0.0	2.373753408561535E-4	0.0
10	5.934383521403838E-5	1.1868767042807675E-4	0.0	2.373753408561535E-4	0.0
11	5.934383521403838E-5	1.7803150564211514E-4	0.0	3.560630112842303E-4	0.0
12	5.934383521403838E-5	1.7803150564211514E-4	0.0	5.340945169263454E-4	0.0
13	5.934383521403838E-5	1.7803150564211514E-4	0.0	6.527821873544222E-4	0.0
14	5.934383521403838E-5	2.373753408561535E-4	0.0	7.714698577824989E-4	0.0
15	5.934383521403838E-5	2.373753408561535E-4	0.0	9.49501363424614E-4	0.0
16	5.934383521403838E-5	2.373753408561535E-4	0.0	0.0011275328690667293	0.0
17	5.934383521403838E-5	2.373753408561535E-4	0.0	0.001720971221207113	0.0
18	5.934383521403838E-5	2.373753408561535E-4	0.0	0.0022550657381334585	0.0
19	5.934383521403838E-5	2.373753408561535E-4	0.0	0.002670472584631727	0.0
20	5.934383521403838E-5	2.373753408561535E-4	0.0	0.005044225993193262	0.0
21	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.009613701304674217	0.0
22	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.016319554683860555	0.0
23	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.02053296698405728	0.0
24	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.026111287494176887	0.0
25	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.02961257377180515	0.0
26	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.03614039564534937	0.0
27	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.07180604060898643	0.0
28	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.14473961408703961	0.0
29	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.23310258472074274	0.0
30	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.36484589889590796	0.0
31	1.1868767042807675E-4	2.373753408561535E-4	0.0	0.5712437577703334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3590	0.0	30.043177	1
GTATTGG	490	0.0	19.255104	1
GTATCAA	5700	0.0	18.954386	2
TCTAACG	50	0.0070362105	18.5	2
GGGTAAG	215	0.0	16.348839	1
TATACCG	140	3.475907E-8	15.857142	5
TAATACT	685	0.0	15.124087	4
GGACCCT	785	0.0	15.082803	6
CGCCTAC	75	0.0041054618	14.8	7
CTAGTAC	125	2.9605435E-6	14.799999	3
TTGGACC	870	0.0	14.459769	4
TCTAATA	585	0.0	14.23077	2
CTCTAAT	445	0.0	14.134832	1
GTCTAAC	105	1.6563539E-4	14.095238	1
GACCCTC	840	0.0	14.095238	7
TACCGCA	200	9.840733E-10	13.875001	7
TGGACCC	840	0.0	13.875	5
TATTGCG	120	3.3021537E-5	13.874999	9
CGAACTA	390	0.0	13.756411	24
TAGTACC	175	3.5683115E-8	13.742857	4
>>END_MODULE