FastQCFastQC Report
Wed 25 May 2016
SRR2088079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078626
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102510.9503757558226855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101960.9452766760675154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73440.6808662131267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37580.34840621308961583No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG21090.19552653097551884No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA20740.19228166204041067No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG18660.17299786951176774No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16600.153899498065131No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16170.1499129448019981No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.1221924930420739No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA12620.1170007027459008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.11431209705681117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.025.9333321
GCGAACT652.6825928E-622.76922823
ACGTTTA957.1431714E-921.42105326
CGGTATA957.1431714E-921.42105326
CGAACTA801.6165199E-518.524
ACGCCTA801.6165199E-518.56
TAACGCC609.2363125E-418.54
TCTAACG609.2363125E-418.52
AGCGAAC801.6165199E-518.522
GTACTAG1306.9667294E-1018.4999981
GTATTGG1502.5102054E-1017.2666661
GTATACG650.001579849217.0769211
TACTATA3400.016.8676452
ACGAGTC904.4455475E-516.44444526
ACTGCGT700.002592354515.8571436
CTAACGC700.002592354515.8571433
GCTTACG700.002592354515.8571432
ATTGGAC1751.3096724E-1015.8571433
GTATCAA45650.015.683462
GTCTAAC957.059032E-515.5789481
Produced by FastQC (version 0.11.5)