FastQCFastQC Report
Wed 25 May 2016
SRR2088078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492485
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT572621.6395775500825345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT539301.5441727022449632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT420871.2050731785533797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205080.5872036673027944No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG138150.3955636173097379No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA130370.37328721526363035No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG128960.36924997530411724No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG116060.3323135246106998No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA113550.3251266648246163No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86460.24756011836843966No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA80150.22949275372693081No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66920.19161141708554225No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC64130.18362283588905895No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60470.17314319173883352No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59670.17085255913769137No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA59060.16910595177932045No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58540.16761704058857804No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48330.13838284201650114No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47920.13720889280841578No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT46740.13383020972173107No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.13299985540381706No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA44690.1279604636813043No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43620.12489674257727663No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC42060.12043000900504941No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41330.11833980675650718No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA38940.11149654186059497No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT36820.10542636546756823No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA36540.10462464405716847No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36300.10393745427682581No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG36090.103336163219026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152400.026.9124031
GTATTGG8900.020.7865181
ATTGGAC9350.019.7860953
TTGGACC15400.018.6201294
CGCGAAT609.2419545E-418.535
AGCCGTC2800.017.17857212
GTATCAA246800.016.6559982
TATTGGA12050.015.6597512
GCCGTCA3000.015.41666713
CTAGTAC4100.015.3414633
TGGACCC18600.015.31720355
TTTTTAC24850.015.1871221
GGACCCT17950.015.0473546
GACCCTC17650.014.9886687
TATACCG2600.014.94230655
GTACTAG3600.014.9027781
TCACGTT3000.014.824
TATACTG4300.014.6279085
TAGGACG1651.6483682E-814.5757574
CTACGTT908.279973E-414.3888884
Produced by FastQC (version 0.11.5)