##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3492485
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62792939697665	31.0	31.0	34.0	30.0	34.0
2	31.825726094743427	31.0	31.0	34.0	30.0	34.0
3	31.881566277306845	31.0	31.0	34.0	30.0	34.0
4	35.57302436517265	37.0	35.0	37.0	33.0	37.0
5	35.48549299424335	37.0	35.0	37.0	33.0	37.0
6	35.53451195924965	37.0	35.0	37.0	33.0	37.0
7	35.379174141048566	37.0	35.0	37.0	33.0	37.0
8	35.44095479293397	37.0	35.0	37.0	33.0	37.0
9	36.97689925654656	39.0	37.0	39.0	33.0	39.0
10	36.84236839957795	39.0	37.0	39.0	32.0	39.0
11	36.89618623988363	39.0	37.0	39.0	33.0	39.0
12	36.764827050080385	39.0	37.0	39.0	32.0	39.0
13	36.794117369151195	39.0	37.0	39.0	32.0	39.0
14	37.742500826775206	40.0	37.0	41.0	32.0	41.0
15	37.760702766082034	39.0	37.0	41.0	32.0	41.0
16	37.64299660556881	39.0	36.0	41.0	32.0	41.0
17	37.70538857002965	39.0	37.0	41.0	32.0	41.0
18	37.71012932052679	39.0	37.0	41.0	32.0	41.0
19	37.7682146093684	40.0	37.0	41.0	32.0	41.0
20	37.71055108325447	40.0	37.0	41.0	32.0	41.0
21	37.63143692814715	39.0	37.0	41.0	32.0	41.0
22	37.51565547167704	39.0	36.0	41.0	32.0	41.0
23	37.301834939878056	39.0	36.0	41.0	31.0	41.0
24	37.14279259610277	39.0	36.0	41.0	31.0	41.0
25	36.978822242615216	39.0	36.0	41.0	31.0	41.0
26	37.13456722076115	39.0	36.0	41.0	31.0	41.0
27	37.05456143691383	39.0	36.0	41.0	31.0	41.0
28	36.96438839393727	39.0	35.0	41.0	31.0	41.0
29	36.853202232794125	39.0	35.0	41.0	31.0	41.0
30	36.57128634768653	39.0	35.0	40.0	30.0	41.0
31	36.30042018791777	38.0	35.0	40.0	30.0	41.0
32	36.11540751069797	38.0	35.0	40.0	30.0	41.0
33	35.91383670939174	38.0	35.0	40.0	30.0	41.0
34	35.76879356675834	38.0	35.0	40.0	29.0	41.0
35	35.53757596668275	38.0	34.0	40.0	27.0	41.0
36	35.40690854792504	38.0	34.0	40.0	27.0	41.0
37	35.19517134647679	38.0	34.0	40.0	25.0	41.0
38	35.05090329664981	38.0	34.0	40.0	25.0	41.0
39	34.88735069728288	38.0	34.0	40.0	24.0	41.0
40	34.64221177757385	38.0	33.0	40.0	23.0	41.0
41	34.409735188554855	38.0	33.0	40.0	22.0	41.0
42	34.135638377831256	38.0	33.0	40.0	20.0	41.0
43	33.75815558262956	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	1.0
13	3.0
14	7.0
15	12.0
16	52.0
17	100.0
18	211.0
19	587.0
20	1202.0
21	2499.0
22	4821.0
23	8371.0
24	13674.0
25	21382.0
26	31058.0
27	43612.0
28	58676.0
29	76799.0
30	96579.0
31	118876.0
32	147500.0
33	183225.0
34	226966.0
35	276384.0
36	360687.0
37	501620.0
38	626612.0
39	690965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18027278570989	22.175814642009918	15.509357949998353	22.134554622281843
2	18.72154067948753	24.25138547481235	35.452722058935116	21.57435178676501
3	19.362803276177278	24.435953196649375	34.546232839940615	21.65501068723273
4	13.499671437386274	16.739799884609383	36.055645192463246	33.704883485541096
5	11.72780985458778	39.41643271195152	34.87937671886923	13.976380714591471
6	31.283341231243654	37.757957442909564	12.905538606465026	18.053162719381756
7	24.35684047318743	34.963299770793576	21.05137173101674	19.628488025002255
8	27.078656028587094	34.815697132557474	18.537574248708296	19.568072590147132
9	23.904469167369367	14.890973046412512	23.03004880479086	38.174508981427266
10	16.413671068021767	28.800381390328088	32.90024151857489	21.88570602307526
11	31.86178322884708	23.167085900154188	24.017111025530532	20.954019845468196
12	21.06296805856002	28.399492052220698	30.92250933074874	19.615030558470544
13	32.121884560706775	21.774610341919864	26.940072756217994	19.163432341155367
14	19.774229524249925	24.45510861177643	29.03402591564459	26.736635948329056
15	21.639634815897562	30.609809347785315	26.66611309712139	21.08444273919573
16	20.552013823967748	29.335043672342188	27.83662635630504	22.276316147385028
17	20.75719723921506	27.754678974999177	27.59015428842214	23.897969497363626
18	22.702803304810185	25.703675176843994	30.870311540350208	20.72320997799561
19	21.981941225230745	24.999563348160407	32.858294309066466	20.160201117542382
20	23.043534904230082	24.270254560864256	32.63358325089442	20.05262728401124
21	21.667036508388726	25.40159800256837	33.48234852834013	19.449016960702767
22	19.704680191897747	25.797476581860767	32.984622697019454	21.51322052922203
23	21.385660926245926	25.90018282111448	33.7451986193212	18.968957633318396
24	20.815407940191584	25.99063417595208	31.351888411832835	21.8420694720235
25	20.74436969664866	26.743822808115137	32.44288808684934	20.068919408386865
26	20.332141727165613	27.605444261034766	32.26361745290245	19.798796558897173
27	18.96669563362477	27.518371589283845	31.91813279083518	21.596799986256205
28	19.76019939956793	27.005298519535515	31.834381536355917	21.400120544540634
29	19.94811717158413	26.657666389404678	31.089925940984713	22.30429049802648
30	20.389436175101682	27.187203380973717	33.083578025388796	19.3397824185358
31	21.665547597197985	26.859413855750276	32.3979057891444	19.077132757907336
32	20.932373367387406	26.84063066842091	31.42327025026593	20.803725713925758
33	20.225970906102674	26.838683630709937	32.144361393105484	20.790984070081905
34	19.153095861542713	27.284211671632093	33.12598336141745	20.436709105407754
35	18.67638658433751	29.693928535126133	31.73725871406749	19.89242616646886
36	21.49870364511229	26.86840458870976	31.50676380857756	20.12612795760039
37	20.46674502539023	27.892718222125506	31.435009742346782	20.20552701013748
38	20.089477835982116	26.22525222012407	32.982704292216006	20.70256565167782
39	19.648846022244907	25.71080477081505	33.80867777528035	20.831671431659693
40	18.148023541976556	26.217320904742614	33.34173231953752	22.29292323374331
41	19.95653524639333	25.414396912227254	33.378239276618224	21.250828564761196
42	18.584016824696455	27.760262391964464	32.40460588950275	21.251114893836338
43	18.816716464064985	27.970628363471857	31.411530758185073	21.801124414278085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	521.0
1	648.0
2	775.0
3	1403.5
4	2032.0
5	2032.0
6	3667.0
7	5302.0
8	6298.5
9	7295.0
10	12262.0
11	17229.0
12	17229.0
13	33584.0
14	49939.0
15	81885.0
16	113831.0
17	104753.5
18	95676.0
19	95676.0
20	104484.0
21	113292.0
22	89759.0
23	66226.0
24	73642.0
25	81058.0
26	81058.0
27	86502.5
28	91947.0
29	94597.0
30	97247.0
31	104154.0
32	111061.0
33	111061.0
34	122295.0
35	133529.0
36	144554.0
37	155579.0
38	160755.0
39	165931.0
40	165931.0
41	170191.0
42	174451.0
43	184319.0
44	194187.0
45	213463.5
46	232740.0
47	232740.0
48	321534.5
49	410329.0
50	368883.5
51	327438.0
52	288577.0
53	249716.0
54	249716.0
55	215228.0
56	180740.0
57	149297.5
58	117855.0
59	104748.5
60	91642.0
61	91642.0
62	79616.0
63	67590.0
64	58228.5
65	48867.0
66	40579.0
67	32291.0
68	32291.0
69	27326.5
70	22362.0
71	18208.0
72	14054.0
73	11380.0
74	8706.0
75	8706.0
76	6859.5
77	5013.0
78	3920.5
79	2828.0
80	2185.5
81	1543.0
82	1543.0
83	1167.0
84	791.0
85	588.5
86	386.0
87	307.0
88	228.0
89	228.0
90	189.5
91	151.0
92	105.5
93	60.0
94	43.5
95	27.0
96	27.0
97	19.5
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3492485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.03092898288015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0033800745564	24.522834359533526
2	13.244082368794668	9.279050178093222
3	5.9578399117589855	6.261260005205939
4	3.2685791739533427	4.580054596707224
5	1.9176610924508748	3.3588724771439473
6	1.2186696156862238	2.5614677256418767
7	0.882023438184576	2.162867029699576
8	0.5968297131242317	1.6725999436262158
9	0.4353357412806706	1.3725193892861391
>10	2.138809482692843	13.41343704366558
>50	0.17960891403803703	4.412752573974699
>100	0.13609516153525397	9.16396675431704
>500	0.011322074117768626	2.7350014370011726
>1k	0.008368489565206753	6.167455400419211
>5k	6.563521227613138E-4	1.5387814013693366
>10k+	7.383961381064781E-4	6.797079684315327
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57262	1.6395775500825345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	53930	1.5441727022449632	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	42087	1.2050731785533797	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20508	0.5872036673027944	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	13815	0.3955636173097379	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	13037	0.37328721526363035	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12896	0.36924997530411724	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11606	0.3323135246106998	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11355	0.3251266648246163	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8646	0.24756011836843966	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	8015	0.22949275372693081	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6692	0.19161141708554225	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6413	0.18362283588905895	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6047	0.17314319173883352	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5967	0.17085255913769137	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5906	0.16910595177932045	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5854	0.16761704058857804	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4833	0.13838284201650114	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4792	0.13720889280841578	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4674	0.13383020972173107	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4645	0.13299985540381706	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4469	0.1279604636813043	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4362	0.12489674257727663	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4206	0.12043000900504941	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4133	0.11833980675650718	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3894	0.11149654186059497	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3682	0.10542636546756823	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3654	0.10462464405716847	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3630	0.10393745427682581	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3609	0.103336163219026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8632907514277085E-5	0.0
3	0.0	0.0	0.0	5.726581502855417E-5	0.0
4	0.0	0.0	0.0	5.726581502855417E-5	0.0
5	2.8632907514277085E-5	0.0	0.0	5.726581502855417E-5	0.0
6	2.8632907514277085E-5	0.0	0.0	5.726581502855417E-5	0.0
7	2.8632907514277085E-5	0.0	0.0	5.726581502855417E-5	0.0
8	5.726581502855417E-5	0.0	0.0	5.726581502855417E-5	0.0
9	5.726581502855417E-5	0.0	0.0	1.1453163005710834E-4	0.0
10	5.726581502855417E-5	0.0	0.0	2.2906326011421668E-4	0.0
11	5.726581502855417E-5	0.0	0.0	4.867594277427104E-4	0.0
12	5.726581502855417E-5	0.0	0.0	7.730885028854812E-4	0.0
13	5.726581502855417E-5	0.0	0.0	8.589872254283125E-4	0.0
14	5.726581502855417E-5	0.0	0.0	0.001030784670513975	0.0
15	5.726581502855417E-5	0.0	0.0	0.001317113745656746	0.0
16	5.726581502855417E-5	0.0	0.0	0.0018325060809137334	0.0
17	5.726581502855417E-5	0.0	0.0	0.0028060249363991542	0.0
18	5.726581502855417E-5	0.0	0.0	0.003521847624256081	0.0
19	8.589872254283125E-5	0.0	0.0	0.004352201942170117	0.0
20	8.589872254283125E-5	0.0	0.0	0.008017214103997583	0.0
21	8.589872254283125E-5	0.0	0.0	0.013428833624195953	0.0
22	8.589872254283125E-5	0.0	0.0	0.022047338785993353	0.0
23	8.589872254283125E-5	0.0	0.0	0.026370907820649193	0.0
24	8.589872254283125E-5	0.0	0.0	0.033987261219446896	0.0
25	8.589872254283125E-5	0.0	0.0	0.03962794399975948	0.0
26	8.589872254283125E-5	0.0	0.0	0.049792626167327846	0.0
27	8.589872254283125E-5	0.0	0.0	0.11765261697616454	0.0
28	8.589872254283125E-5	0.0	0.0	0.24916356118923919	0.0
29	8.589872254283125E-5	0.0	0.0	0.41821224715353106	0.0
30	8.589872254283125E-5	0.0	0.0	0.7005613481518174	0.0
31	8.589872254283125E-5	0.0	0.0	1.0285799366353756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15240	0.0	26.912403	1
GTATTGG	890	0.0	20.786518	1
ATTGGAC	935	0.0	19.786095	3
TTGGACC	1540	0.0	18.620129	4
CGCGAAT	60	9.2419545E-4	18.5	35
AGCCGTC	280	0.0	17.178572	12
GTATCAA	24680	0.0	16.655998	2
TATTGGA	1205	0.0	15.659751	2
GCCGTCA	300	0.0	15.416667	13
CTAGTAC	410	0.0	15.341463	3
TGGACCC	1860	0.0	15.3172035	5
TTTTTAC	2485	0.0	15.187122	1
GGACCCT	1795	0.0	15.047354	6
GACCCTC	1765	0.0	14.988668	7
TATACCG	260	0.0	14.9423065	5
GTACTAG	360	0.0	14.902778	1
TCACGTT	300	0.0	14.8	24
TATACTG	430	0.0	14.627908	5
TAGGACG	165	1.6483682E-8	14.575757	4
CTACGTT	90	8.279973E-4	14.388888	4
>>END_MODULE