FastQCFastQC Report
Wed 25 May 2016
SRR2088077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455011
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT572731.6576792374901266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT572151.6560005163514673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT435901.2616457661060991No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194900.5641081895253011No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG161460.46732123284122684No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG155160.44908684805923915No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA153210.4434428718171954No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG136040.393746937419302No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA133180.38546910559763775No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA103830.30052002728790156No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA95160.27542604061173753No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC76460.22130175562393287No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA71290.20633798271553982No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA70710.20465926157688064No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA69630.20153336704282562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68020.196873468709651No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61130.17693141932109624No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA59410.17195314284093452No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG58800.17018759129855157No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54060.15646838751019895No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG53400.15455811862827645No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT50900.1473222516512972No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG49910.14445684832841343No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC47860.13852343740729045No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA46890.1357159210202225No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.12790118468508493No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT43200.12503578136220117No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT41440.11994173101040778No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG39750.11505028493396982No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG39680.1148476806586144No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37880.10963785643518936No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT37660.10900110014121517No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA37570.10874060893004392No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT37010.10711977472720058No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36720.10628041415787098No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA35940.10402282366105346No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA35860.10379127591779014No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG35380.10240198945821012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133950.031.3650631
GTATTGG8400.023.3452381
TGCGACG1500.020.96666722
ATTGGAC9200.020.3097843
GTATCAA226600.018.5489862
ACGTTTA2800.018.526
TTGGACC18600.017.70434
AGCCGTC2300.017.69565212
CTGTGCG2650.017.4528319
CAATGCG1950.017.07692319
GGACCCT18600.017.0080646
GACCCTC18500.016.5999987
GTATTAG4300.015.9186061
TACGTAG700.00259390615.85714214
TTTTTAC23750.015.7347361
GTCGTCA1802.0190782E-1015.41666724
GACGTTA2450.015.102047
TCACGTT3450.015.01449224
TGGACCC21300.014.8521135
TATTGGA12600.014.682542
Produced by FastQC (version 0.11.5)