FastQCFastQC Report
Wed 25 May 2016
SRR2088076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5195864
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT669051.2876587993835096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT608991.1720668593327308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT509990.9815306944138645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244000.4696042852545794No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG180650.34768038578376953No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG174760.33634444627496024No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA163590.31484657797047805No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA154060.2965050663373791No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG149500.28772885510475255No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA139390.2682710709903108No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA94170.1812403096000973No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA87100.16763333297407323No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC81640.1571249747876388No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA79410.15283309955764815No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75140.144615024565693No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA71430.13747472990055168No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA62460.12021099859426652No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61310.11799769970884534No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG60190.11584213905521776No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG58970.11349411762894487No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56690.10910601201263158No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG56110.10798973953128872No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55220.1062768386547454No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT52280.100618491938973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154750.028.9305321
GTATTGG18450.021.3577231
ATTGGAC18950.020.4036943
TTGGACC29600.017.3125024
GTATCAA264950.016.8765812
GGACCCT30200.016.8460276
TATACCG3650.016.7260285
CAATGCG3150.015.85714219
TATTGGA24200.015.7479332
GTACTAG4900.015.4795921
TAGGTCG3000.015.41666621
TGGACCC32950.015.3839165
GACCCTC32600.015.0950927
ACGTTTA3600.014.38888926
CGAACTA5550.014.33333324
TGCGACG3100.014.3225822
TTTTTAC32450.014.1386751
TAATACT10500.014.0952384
TAGTACT10300.014.0097084
ATGCGAC4400.013.87521
Produced by FastQC (version 0.11.5)