FastQCFastQC Report
Wed 25 May 2016
SRR2088075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936712
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236432.5240415410499706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT228412.43842290906917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116431.2429647533073134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76040.8117756578329305No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG57830.6173722552929823No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA57450.6133155121317971No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG37550.4008702781644732No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG32530.3472785658772387No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.29592873796855385No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.29400712278693986No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA24240.25877751112401676No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT22380.23892082091400557No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20760.22162628427947972No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18650.19910068409500467No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16860.17999128867784336No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT16420.17529400712278695No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA15530.16579268761369556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC15340.163764316033103No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.16344404683616737No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14570.1555440733117543No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC14490.15469002211992586No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA14490.15469002211992586No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG13770.14700356139346993No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13030.13910358786905688No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG12960.138356293076207No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.1376089982833571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC11150.11903338486108858No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA11070.11817933366926013No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10820.11551042369479626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10300.10995909094791141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC10180.10867801416016877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC10100.10782396296834032No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT9780.10440775820102657No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG9650.10301992501430535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9580.10227263022145547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.031.4625851
GCTCGGT250.005495830429.612
CAATCGT701.9221261E-723.78571514
GTATCAA74700.021.6700132
CCGAATT1251.8189894E-1120.7214
TGTACCC450.003825287620.5555553
TCACGTA450.003825287620.55555525
CGAATTA1303.274181E-1119.92307715
CGGGATA759.262769E-619.73333424
CCCGAAT1500.019.73333413
TCGTTTC851.2448036E-619.58823617
GCCCCGA1355.638867E-1119.18518411
GATACTG2800.019.16071536
AGCCGTC801.6161503E-518.512
ATCTCGT500.00703393418.57
CGAGTAG500.00703393418.53
CGACTAT500.00703393418.536
TATACCG1103.8493454E-818.55
TACGTTA609.2350744E-418.519
CCGACCG1750.017.971439
Produced by FastQC (version 0.11.5)