##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936712
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.599140397475423	31.0	30.0	31.0	28.0	34.0
2	30.78684270085149	31.0	31.0	33.0	27.0	34.0
3	30.626102793601447	31.0	30.0	34.0	27.0	34.0
4	34.41922277071288	35.0	35.0	37.0	30.0	37.0
5	34.156672488448955	35.0	35.0	37.0	30.0	37.0
6	34.47219743101402	35.0	35.0	37.0	30.0	37.0
7	34.27597062917951	35.0	35.0	37.0	30.0	37.0
8	34.463614216536136	35.0	35.0	37.0	30.0	37.0
9	35.754050337777244	37.0	35.0	39.0	30.0	39.0
10	35.440496118337336	37.0	35.0	39.0	30.0	39.0
11	35.69411729539068	37.0	35.0	39.0	30.0	39.0
12	35.40893358897932	37.0	35.0	39.0	30.0	39.0
13	35.592927175054875	37.0	35.0	39.0	30.0	39.0
14	36.1332672155369	38.0	34.0	40.0	29.0	41.0
15	36.28774265729488	38.0	34.0	40.0	30.0	41.0
16	35.98877029439144	38.0	34.0	40.0	29.0	41.0
17	36.20041485536643	38.0	34.0	40.0	30.0	41.0
18	36.222049039619435	38.0	35.0	40.0	30.0	41.0
19	36.309921299182676	38.0	35.0	40.0	30.0	41.0
20	36.254150688792286	38.0	34.0	40.0	29.0	41.0
21	36.18054748951652	38.0	34.0	40.0	29.0	41.0
22	36.04479818770337	38.0	34.0	40.0	29.0	41.0
23	35.77942206355849	38.0	34.0	40.0	27.0	41.0
24	35.60658345361221	38.0	34.0	40.0	27.0	41.0
25	35.4383887470215	38.0	34.0	40.0	27.0	41.0
26	35.43130118969331	38.0	34.0	40.0	27.0	41.0
27	35.26258124161962	38.0	34.0	40.0	27.0	41.0
28	35.098262859875824	37.0	33.0	40.0	27.0	41.0
29	34.977186157538284	37.0	33.0	40.0	26.0	41.0
30	34.63044350878392	37.0	33.0	40.0	25.0	41.0
31	34.29539709110164	37.0	33.0	39.0	25.0	40.0
32	34.07472307390105	36.0	33.0	39.0	24.0	40.0
33	33.8077658874873	36.0	33.0	39.0	24.0	40.0
34	33.577413335155306	35.0	32.0	39.0	23.0	40.0
35	33.30069541118294	35.0	31.0	39.0	22.0	40.0
36	33.13245266421269	35.0	31.0	39.0	21.0	40.0
37	32.827096268650344	35.0	31.0	39.0	19.0	40.0
38	32.57645039243652	35.0	31.0	39.0	18.0	40.0
39	32.298564553459336	35.0	30.0	39.0	16.0	40.0
40	32.01393277763069	35.0	30.0	39.0	15.0	40.0
41	31.722747226468755	35.0	30.0	39.0	13.0	40.0
42	31.35877195979127	35.0	28.0	39.0	10.0	40.0
43	30.91785949149792	35.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	0.0
13	5.0
14	11.0
15	24.0
16	59.0
17	154.0
18	302.0
19	602.0
20	1242.0
21	2202.0
22	3676.0
23	5891.0
24	8962.0
25	12389.0
26	16918.0
27	22281.0
28	28394.0
29	35390.0
30	42397.0
31	50382.0
32	59334.0
33	69644.0
34	81893.0
35	97524.0
36	118722.0
37	129903.0
38	107074.0
39	41332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.711804695573456	26.014185790296267	18.130439238527956	18.14357027560232
2	23.726182647387883	27.26846672189531	29.50512003689501	19.500230593821794
3	24.193455405717017	24.010581694266754	34.05625208174978	17.739710818266445
4	18.90869338708162	20.273573948022445	30.650509441535927	30.167223223360008
5	10.79734219269103	42.89621569916901	33.08327426145923	13.223167846680731
6	31.234680456746577	38.269073098241506	11.723347197431014	18.7728992475809
7	22.368454765178626	37.02835022931274	20.530002818368935	20.073192187139696
8	28.401152115057776	36.42400225469515	17.4800792559506	17.694766374296474
9	23.787674333199533	14.212479396014995	22.728330586135336	39.27151568465014
10	17.718679807667673	30.988286687904072	30.9672556773053	20.325777827122955
11	32.755852385792004	22.53862446515044	22.80444789860704	21.90107525045051
12	19.133202094133523	31.182156308449127	31.194860319927574	18.489781277489772
13	33.87978375423823	21.075421260750367	27.781965000982154	17.262829984029242
14	18.607320072765162	25.01868236982125	30.9582881397911	25.41570941762249
15	22.270238878118352	31.008463647311018	27.764883977145587	18.956413497425036
16	18.90954743827345	28.63356079563409	30.594248819274227	21.86264294681823
17	20.396770832443696	28.137997591575637	30.01488184201761	21.450349733963055
18	21.332170400293794	24.369283194834697	32.94897471154421	21.3495716933273
19	21.69813133599228	23.631703234291866	36.20088138082997	18.46928404888589
20	21.725033948534875	23.222505957007066	36.25180418314274	18.800655911315324
21	20.686294186473535	23.975885864598723	37.58273620920838	17.75508373971936
22	18.267087429220506	24.38412233429272	36.98853009249375	20.36026014399303
23	20.094329954137454	24.587386517947884	38.01050909991545	17.307774427999213
24	19.45902262381607	24.58268923639283	35.669448026714726	20.28884011307638
25	19.65940438469882	25.836329629597998	35.89790672052883	18.606359265174355
26	19.124875095013195	26.955990742085078	35.449102819222986	18.470031343678738
27	18.20431466662112	26.41580336325359	35.46148656150449	19.918395408620793
28	18.537608144232166	26.996344660898973	35.513903953402966	18.952143241465894
29	18.817523422353936	24.862818027312557	34.79970364423644	21.519954906097073
30	19.392299874454473	26.1285218936023	36.48218449213846	17.996993739804765
31	20.10500559403531	26.03948705685419	35.697738472444044	18.157768876666466
32	19.819966008762567	25.676301787529145	35.41942454030695	19.084307663401347
33	19.083667125007473	25.955256258060107	35.73414240449573	19.226934212436696
34	18.124781149382095	26.311715874249504	36.45869808436318	19.104804892005227
35	17.538902031787785	28.34190231362468	35.31629785889366	18.802897795693873
36	20.879950294220638	25.396279753008393	34.464381795044794	19.259388157726175
37	19.63570446412558	27.030720221370068	34.194821887623945	19.138753426880406
38	19.938785880825698	24.680584854256164	35.52041609374066	19.86021317117748
39	18.631019993338402	24.877870679568534	36.56566799613969	19.92544133095338
40	17.432679414804124	25.280128790919726	35.424335334659965	21.86285645961619
41	19.39934579678706	24.571586570899058	35.35974771327793	20.669319919035946
42	18.863962455909608	26.785500772916325	33.588445541425756	20.76209122974831
43	19.205155907045068	26.811869603464032	32.402808974369925	21.580165515120978
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	150.0
2	171.0
3	262.5
4	354.0
5	354.0
6	789.5
7	1225.0
8	1550.5
9	1876.0
10	3362.5
11	4849.0
12	4849.0
13	11173.0
14	17497.0
15	30460.0
16	43423.0
17	37514.5
18	31606.0
19	31606.0
20	38079.5
21	44553.0
22	33789.0
23	23025.0
24	23779.5
25	24534.0
26	24534.0
27	24482.5
28	24431.0
29	24804.0
30	25177.0
31	26585.5
32	27994.0
33	27994.0
34	30705.5
35	33417.0
36	36155.0
37	38893.0
38	40133.5
39	41374.0
40	41374.0
41	43435.5
42	45497.0
43	47655.5
44	49814.0
45	54883.0
46	59952.0
47	59952.0
48	86531.5
49	113111.0
50	99483.0
51	85855.0
52	73092.0
53	60329.0
54	60329.0
55	50808.5
56	41288.0
57	34634.0
58	27980.0
59	24525.0
60	21070.0
61	21070.0
62	18282.0
63	15494.0
64	13369.0
65	11244.0
66	9381.5
67	7519.0
68	7519.0
69	6316.0
70	5113.0
71	4072.0
72	3031.0
73	2563.5
74	2096.0
75	2096.0
76	1631.0
77	1166.0
78	940.0
79	714.0
80	566.5
81	419.0
82	419.0
83	327.5
84	236.0
85	173.5
86	111.0
87	92.5
88	74.0
89	74.0
90	59.0
91	44.0
92	31.0
93	18.0
94	11.0
95	4.0
96	4.0
97	3.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	936712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.28178442878544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7330733865042	37.364706987475024
2	10.650637570220326	9.858610241081205
3	3.44788121361929	4.787222851943613
4	1.5109091144754787	2.797102797105649
5	0.8186830781081678	1.894505686824837
6	0.5151865164304532	1.4306250776430698
7	0.34094581029247983	1.1045706345697892
8	0.2519603115442706	0.9328938258801243
9	0.1929465410291588	0.80369191963722
>10	1.308592763590571	11.868316061917232
>50	0.13473267898154	4.378889008765544
>100	0.07963516968427738	7.226674873216683
>500	0.007407922759906394	2.446248194045843
>1k	0.006018937242423945	4.828318801010683
>5k	6.944927587412244E-4	2.0498257028944056
>10k+	6.944927587412244E-4	6.227797335989082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23643	2.5240415410499706	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22841	2.43842290906917	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11643	1.2429647533073134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7604	0.8117756578329305	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5783	0.6173722552929823	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5745	0.6133155121317971	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3755	0.4008702781644732	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3253	0.3472785658772387	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2772	0.29592873796855385	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2754	0.29400712278693986	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2424	0.25877751112401676	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2238	0.23892082091400557	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2076	0.22162628427947972	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1865	0.19910068409500467	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1686	0.17999128867784336	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1642	0.17529400712278695	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1553	0.16579268761369556	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1534	0.163764316033103	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1531	0.16344404683616737	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1457	0.1555440733117543	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1449	0.15469002211992586	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	1449	0.15469002211992586	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1377	0.14700356139346993	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1303	0.13910358786905688	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1296	0.138356293076207	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.1376089982833571	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	1115	0.11903338486108858	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1107	0.11817933366926013	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1082	0.11551042369479626	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1030	0.10995909094791141	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	1018	0.10867801416016877	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	1010	0.10782396296834032	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	978	0.10440775820102657	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	965	0.10301992501430535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	958	0.10227263022145547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0675639897855478E-4	0.0
5	2.1351279795710955E-4	0.0	0.0	1.0675639897855478E-4	0.0
6	2.1351279795710955E-4	0.0	0.0	1.0675639897855478E-4	0.0
7	2.1351279795710955E-4	0.0	0.0	1.0675639897855478E-4	0.0
8	2.1351279795710955E-4	0.0	0.0	1.0675639897855478E-4	0.0
9	2.1351279795710955E-4	0.0	0.0	1.0675639897855478E-4	0.0
10	3.202691969356643E-4	0.0	0.0	1.0675639897855478E-4	0.0
11	3.202691969356643E-4	0.0	0.0	1.0675639897855478E-4	0.0
12	3.202691969356643E-4	0.0	0.0	3.202691969356643E-4	0.0
13	3.202691969356643E-4	0.0	0.0	4.270255959142191E-4	0.0
14	3.202691969356643E-4	0.0	0.0	5.337819948927738E-4	0.0
15	3.202691969356643E-4	0.0	0.0	8.540511918284382E-4	0.0
16	3.202691969356643E-4	0.0	0.0	0.0012810767877426573	0.0
17	3.202691969356643E-4	0.0	0.0	0.001921615181613986	0.0
18	3.202691969356643E-4	0.0	0.0	0.0021351279795710954	0.0
19	3.202691969356643E-4	0.0	0.0	0.002882422772420979	0.0
20	3.202691969356643E-4	0.0	0.0	0.005658089145863403	0.0
21	3.202691969356643E-4	0.0	0.0	0.011743203887641026	0.0
22	3.202691969356643E-4	0.0	0.0	0.0167607546396331	0.0
23	3.202691969356643E-4	0.0	0.0	0.020283715805925407	0.0
24	3.202691969356643E-4	0.0	0.0	0.027329638138510023	0.0
25	3.202691969356643E-4	0.0	0.0	0.03085259930480233	0.0
26	3.202691969356643E-4	0.0	0.0	0.03864581643023683	0.0
27	3.202691969356643E-4	0.0	0.0	0.08689970876854358	0.0
28	3.202691969356643E-4	0.0	0.0	0.18180614746047877	0.0
29	3.202691969356643E-4	0.0	0.0	0.300198993927696	0.0
30	3.202691969356643E-4	0.0	0.0	0.4987658960278079	0.0
31	3.202691969356643E-4	0.0	0.0	0.7302137690133147	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5145	0.0	31.462585	1
GCTCGGT	25	0.0054958304	29.6	12
CAATCGT	70	1.9221261E-7	23.785715	14
GTATCAA	7470	0.0	21.670013	2
CCGAATT	125	1.8189894E-11	20.72	14
TGTACCC	45	0.0038252876	20.555555	3
TCACGTA	45	0.0038252876	20.555555	25
CGAATTA	130	3.274181E-11	19.923077	15
CGGGATA	75	9.262769E-6	19.733334	24
CCCGAAT	150	0.0	19.733334	13
TCGTTTC	85	1.2448036E-6	19.588236	17
GCCCCGA	135	5.638867E-11	19.185184	11
GATACTG	280	0.0	19.160715	36
AGCCGTC	80	1.6161503E-5	18.5	12
ATCTCGT	50	0.007033934	18.5	7
CGAGTAG	50	0.007033934	18.5	3
CGACTAT	50	0.007033934	18.5	36
TATACCG	110	3.8493454E-8	18.5	5
TACGTTA	60	9.2350744E-4	18.5	19
CCGACCG	175	0.0	17.97143	9
>>END_MODULE