FastQCFastQC Report
Mon 6 Feb 2017
SRR3203983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634773
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17400.042.425221
TCAACGC24350.030.0319274
TATCAAC24500.029.5664752
CAACGCA24850.029.5664755
ATCAACG24950.029.3097173
GGTATCA8450.028.9841941
AACGCAG25350.027.8947336
TATACCG809.575152E-525.8706655
CGCGTAA700.001335390624.6387310
GCGTAAC750.001995323522.99614711
GTACATG48800.022.8319131
ACGCAGA31100.021.9609497
TACATGG49700.021.7237242
GTATTAC1605.7303623E-821.5595471
ACATGGG50900.020.872733
GTATAGA2650.020.8273351
TATCGTT850.004124915720.29071637
CGCAGAG33650.019.8867138
AGAGTAC32800.019.45558211
GTATAAA3200.019.4035931
Produced by FastQC (version 0.11.5)