##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3203983_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1634773 Sequences flagged as poor quality 0 Sequence length 75 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50572954165502 35.0 31.0 35.0 15.0 35.0 2 31.486204506680743 35.0 31.0 35.0 15.0 35.0 3 31.402096193171772 35.0 31.0 35.0 15.0 35.0 4 31.68852311605342 35.0 31.0 35.0 15.0 35.0 5 31.623159301016106 35.0 31.0 35.0 15.0 35.0 6 35.14615240158725 39.0 34.0 40.0 16.0 40.0 7 34.79088350492698 39.0 34.0 40.0 16.0 40.0 8 34.77498588489044 39.0 34.0 40.0 16.0 40.0 9 34.79446198340687 39.0 34.0 40.0 16.0 40.0 10 34.94498074044531 39.0 34.0 40.0 16.0 40.0 11 35.04287567754055 39.0 34.0 40.0 16.0 40.0 12 34.61761541204803 39.0 34.0 40.0 16.0 40.0 13 34.7003229194512 39.0 34.0 40.0 16.0 40.0 14 34.67724509763741 39.0 34.0 40.0 16.0 40.0 15 34.7380822903241 39.0 34.0 40.0 16.0 40.0 16 34.89355586371931 39.0 34.0 40.0 16.0 40.0 17 34.93186515803723 39.0 34.0 40.0 16.0 40.0 18 34.986281275748986 39.0 34.0 40.0 16.0 40.0 19 35.03025312994526 39.0 34.0 40.0 16.0 40.0 20 34.987578703587594 39.0 34.0 40.0 16.0 40.0 21 35.01233382249401 39.0 34.0 40.0 16.0 40.0 22 35.07034860497451 39.0 34.0 40.0 16.0 40.0 23 35.0899244115238 39.0 34.0 40.0 16.0 40.0 24 35.10155783096491 39.0 34.0 40.0 16.0 40.0 25 35.12661696761569 39.0 34.0 40.0 16.0 40.0 26 35.134486561742825 39.0 34.0 40.0 16.0 40.0 27 35.130739864189096 39.0 34.0 40.0 16.0 40.0 28 34.997501793827034 39.0 34.0 40.0 16.0 40.0 29 35.08233008497204 39.0 34.0 40.0 16.0 40.0 30 35.10523968771199 39.0 34.0 40.0 16.0 40.0 31 35.097255092908924 39.0 34.0 40.0 16.0 40.0 32 35.039289858591985 39.0 34.0 40.0 16.0 40.0 33 34.92003782788191 39.0 34.0 40.0 16.0 40.0 34 34.958983907857544 39.0 34.0 40.0 16.0 40.0 35 34.93955858091613 39.0 34.0 40.0 16.0 40.0 36 34.95361924866633 39.0 34.0 40.0 16.0 40.0 37 34.916169400889295 39.0 34.0 40.0 16.0 40.0 38 34.93366724309736 39.0 34.0 40.0 16.0 40.0 39 34.88171507603808 39.0 34.0 40.0 16.0 40.0 40 34.81649378843424 39.0 34.0 40.0 16.0 40.0 41 34.771671051577194 39.0 34.0 40.0 16.0 40.0 42 34.78403912959169 39.0 34.0 40.0 16.0 40.0 43 34.70555606191196 39.0 34.0 40.0 16.0 40.0 44 34.57459047831106 39.0 34.0 40.0 16.0 40.0 45 34.58893069557669 39.0 34.0 40.0 16.0 40.0 46 34.54495639455753 39.0 34.0 40.0 16.0 40.0 47 34.469150762827624 39.0 34.0 40.0 16.0 40.0 48 34.5014524952394 39.0 34.0 40.0 16.0 40.0 49 34.441450280864686 39.0 34.0 40.0 16.0 40.0 50 34.342159431309426 39.0 34.0 40.0 16.0 40.0 51 34.37564726111821 39.0 34.0 40.0 16.0 40.0 52 34.403572850787235 39.0 34.0 40.0 16.0 40.0 53 34.34569325527153 39.0 34.0 40.0 16.0 40.0 54 34.36387253765508 39.0 34.0 40.0 16.0 40.0 55 34.35276885537014 39.0 34.0 40.0 16.0 40.0 56 34.28736405604937 39.0 34.0 40.0 16.0 40.0 57 34.17475759631459 39.0 34.0 40.0 16.0 40.0 58 34.19661751203378 39.0 34.0 40.0 16.0 40.0 59 34.132936499440596 39.0 34.0 40.0 16.0 40.0 60 34.0844753369428 39.0 34.0 40.0 16.0 40.0 61 34.05505045654657 39.0 34.0 40.0 16.0 40.0 62 34.00916824537719 39.0 34.0 40.0 16.0 40.0 63 33.96353193990848 39.0 34.0 40.0 16.0 40.0 64 33.945397312042715 39.0 34.0 40.0 16.0 40.0 65 33.86929500303712 39.0 34.0 40.0 16.0 40.0 66 33.81671094396592 39.0 34.0 40.0 16.0 40.0 67 33.81162155235008 39.0 34.0 40.0 16.0 40.0 68 33.77531253574655 39.0 34.0 40.0 16.0 40.0 69 33.73330731544991 39.0 34.0 40.0 16.0 40.0 70 33.60206646427363 39.0 34.0 40.0 16.0 40.0 71 33.502488724734256 39.0 31.0 40.0 16.0 40.0 72 33.466691094115205 39.0 34.0 40.0 16.0 40.0 73 33.41416820561631 38.0 34.0 40.0 16.0 40.0 74 33.35068171544306 38.0 31.0 40.0 16.0 40.0 75 33.24455811296125 38.0 31.0 40.0 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4960.0 3 1187.0 4 1219.0 5 1231.0 6 1184.0 7 1243.0 8 1347.0 9 1495.0 10 1582.0 11 1719.0 12 1865.0 13 2193.0 14 2606.0 15 2892.0 16 3440.0 17 3949.0 18 4741.0 19 7990.0 20 14141.0 21 19251.0 22 22902.0 23 26003.0 24 28688.0 25 30950.0 26 33778.0 27 36164.0 28 39123.0 29 42094.0 30 46457.0 31 51129.0 32 56285.0 33 63033.0 34 71763.0 35 84663.0 36 105957.0 37 146682.0 38 255732.0 39 413135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.665284160145646 15.079353427392714 11.778099139175586 26.477263273286063 2 16.56462395696528 18.46366437419752 37.41907897916102 27.55263268967618 3 20.032873065557112 22.60980576508176 26.907772516428885 30.44954865293224 4 13.427124132830675 15.193974943310174 34.27900999098957 37.09989093286958 5 15.585038412060879 36.71060141071574 32.09216203105875 15.612198146164635 6 35.66219897196736 33.96844699539324 16.247515710132234 14.12183832250716 7 31.608669827554042 30.06172722451374 19.190370773189915 19.139232174742304 8 28.31928347238424 33.563375465584514 18.374355338631112 19.742985723400132 9 28.254503836312445 14.943726132007317 16.50186294977957 40.29990708190066 10 15.394247397039221 28.184035337016212 31.787532580976073 24.634184684968492 11 37.61665992770862 21.622023363488385 21.390431576738788 19.37088513206421 12 25.63866665280134 23.157160046073674 27.939169536076264 23.265003765048725 13 30.0931688986789 20.138698155646072 23.5218589981606 26.246273947514425 14 23.979843073013807 19.00661437398342 24.87213821123789 32.141404341764876 15 25.504764270024033 26.657951899132172 21.85973220746856 25.977551623375234 16 25.86787278723101 25.92781994809065 22.963922208159786 25.240385056518548 17 24.886513295729742 25.699959566251707 24.28649115198257 25.127035986035985 18 24.79671489558489 25.627044244063242 24.70532606056009 24.870914799791773 19 25.888181417236524 24.96670791602259 24.679940273053198 24.465170393687686 20 26.01217416730029 24.695905792425005 24.65100659235258 24.640913447922127 21 26.221316354013673 24.428101026870397 24.595953077277397 24.75462954183853 22 25.86383552945883 24.262145264204875 24.5364952809962 25.337523925340093 23 25.698919666522507 24.315608344400108 24.489883304899212 25.495588684178173 24 25.15070899751831 24.388584837160877 24.706121278000065 25.754584887320746 25 25.643132104579657 24.291140115477805 24.532825046657855 25.532902733284686 26 25.356303291037964 24.804850581701558 24.67058117549042 25.168264951770063 27 25.486780121766138 24.838372055325113 24.326741388559757 25.34810643434899 28 25.60360355947624 24.79124751878742 24.225206548319512 25.37994237341683 29 25.661238593982162 24.890367041785005 24.28545125225337 25.162943111979462 30 25.748345488945564 24.67388438639493 24.31878921416001 25.258980910499503 31 25.575232769320266 24.421922799067517 24.383813532521028 25.61903089909119 32 25.380037473092592 24.791026032360456 24.386505037702484 25.442431456844467 33 25.551255027822865 24.61130002423637 24.26740061251919 25.570044335421578 34 25.736616482298448 24.714577935027023 24.30828274523909 25.240522837435435 35 25.679222742240054 24.652597027232527 24.461744841638563 25.206435388888853 36 25.700571271974766 24.796531383867972 24.24409994537468 25.258797398782583 37 25.622166271504526 24.530882594025343 24.36462087679505 25.48233025767508 38 25.637004040955198 24.72390476411857 24.38177545110542 25.25731574382081 39 25.78394868021109 24.789068057933566 24.262755166020398 25.164228095834947 40 25.895583056485517 24.421188752199846 24.556559228712487 25.126668962602146 41 25.401723915395895 24.536401823623795 24.64265549769478 25.419218763285535 42 25.56113638490717 24.61989903123572 24.449660287148657 25.36930429670845 43 25.36991419593325 24.31416999689865 24.63641696604601 25.67949884112209 44 25.4557232725947 24.623737738291275 24.472400909244392 25.44813807986963 45 25.51406597049966 24.75132450553934 24.269847396653088 25.464762127307907 46 25.588538088821007 24.623815812734627 24.13004634899934 25.65759974944503 47 25.37220763983746 24.740804992497427 24.27554161953984 25.611445748125274 48 25.302871166664932 24.828798651309572 24.4187106328654 25.449619549160097 49 25.56862634751125 24.48890457574232 24.26716125113395 25.675307825612485 50 25.67231211259817 24.635774228652487 24.441801869254924 25.25011178949442 51 25.45937907743089 24.74930586183546 24.281347548663078 25.50996751207057 52 25.58032699441144 24.741431200023488 24.083539682695037 25.594702122870032 53 25.446745621853147 24.693549600646453 24.362676760663753 25.497028016836648 54 25.256188782014423 24.702703562418794 24.442660937694985 25.5984467178718 55 25.319498785133852 24.433184808912443 24.36326577552014 25.88405063043356 56 25.215871609916512 24.944823975022757 24.342903702543726 25.496400712517005 57 25.416757403515128 24.549048058313005 24.212975083865317 25.821219454306554 58 25.52561167925362 24.39052290180467 24.37700411436038 25.70686130458134 59 25.4791361449505 24.647395803066495 24.269482157986012 25.603985893996995 60 25.395200943515107 24.661994766133777 24.54234256498595 25.400461725365165 61 25.63345129332889 24.417290875891197 24.479258106053887 25.469999724726026 62 25.322787346038677 24.765208029386844 24.377379958342125 25.534624666232347 63 25.143798482094848 24.519116955283152 24.47115863986792 25.86592592275408 64 25.407463413321196 24.432267236295747 24.409389092386107 25.75088025799695 65 25.361110924198055 24.539087699809205 24.322968630619908 25.77683274537283 66 25.274005325646847 24.68614102259026 24.37997444227827 25.65987920948462 67 25.44335877881274 24.537892027891946 24.098494130860193 25.92025506243512 68 25.297970059850933 24.805472225348804 24.47043177825302 25.426125936547244 69 25.588216762533726 24.521128578498335 24.118248345750274 25.772406313217665 70 25.1753979755443 24.338430462632232 24.504943075565038 25.981228486258427 71 25.38224896665082 24.419400599363932 24.420807556952724 25.777542877032516 72 25.087214583660856 24.704345161158116 24.40399544642917 25.80444480875186 73 25.26130667098941 24.436172783244075 24.343621254431064 25.958899291335452 74 25.202662197046628 24.687289721912965 24.24146959149467 25.86857848954574 75 25.295458382576395 24.554182051300874 24.34564838127956 25.80471118484317 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 188.0 1 201.0 2 250.0 3 313.5 4 341.0 5 316.5 6 291.5 7 300.0 8 309.0 9 393.0 10 558.5 11 617.5 12 595.0 13 627.5 14 695.5 15 803.5 16 876.0 17 984.5 18 1216.5 19 1446.5 20 1553.0 21 1822.0 22 2375.0 23 2985.0 24 3311.0 25 3691.0 26 4435.5 27 5192.5 28 5585.0 29 6182.0 30 7355.5 31 8610.0 32 9288.0 33 10029.5 34 11741.5 35 13847.5 36 14983.0 37 16393.5 38 19837.0 39 24873.5 40 27877.0 41 32428.0 42 42552.5 43 53811.5 44 59497.0 45 66355.0 46 79293.5 47 90981.5 48 96589.0 49 100803.0 50 107845.5 51 112012.0 52 113350.0 53 112036.5 54 109613.5 55 104519.0 56 100534.0 57 96939.5 58 86725.0 59 72237.0 60 64369.0 61 58881.5 62 46880.0 63 34826.5 64 29287.0 65 24552.0 66 16710.0 67 11782.5 68 9962.0 69 8356.0 70 5564.5 71 3552.5 72 2726.0 73 2427.0 74 1876.5 75 1274.0 76 923.0 77 709.0 78 374.0 79 193.0 80 133.0 81 95.0 82 42.5 83 20.5 84 13.0 85 9.5 86 5.5 87 4.0 88 3.0 89 1.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003731404910651204 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0916946878863304 29 0.0 30 0.0 31 0.0 32 0.0 33 0.052912545044480175 34 6.117057230575743E-5 35 0.0 36 0.0 37 6.117057230575743E-5 38 6.728762953633318E-4 39 2.446822892230297E-4 40 0.0 41 3.6702343383454464E-4 42 6.117057230575744E-4 43 1.2234114461151486E-4 44 5.50535150751817E-4 45 7.340468676690893E-4 46 1.2234114461151486E-4 47 0.0 48 1.2234114461151486E-4 49 0.0 50 2.446822892230297E-4 51 7.340468676690893E-4 52 3.058528615287872E-4 53 3.6702343383454464E-4 54 0.002507993464536055 55 0.0015292643076439357 56 3.058528615287872E-4 57 5.50535150751817E-4 58 6.728762953633318E-4 59 4.893645784460594E-4 60 0.00214097003070151 61 0.0023244817476187826 62 0.0017127760245612084 63 0.0012234114461151487 64 0.0015292643076439357 65 0.0015904348799496934 66 0.0025691640368418122 67 0.002202140603007268 68 0.0027526757537590847 69 0.002691505181453327 70 0.003792575482956961 71 0.0024468228922302974 72 7.340468676690893E-4 73 4.893645784460594E-4 74 0.0020186288860899954 75 0.0015292643076439357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 1634773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.35109780765033 #Duplication Level Percentage of deduplicated Percentage of total 1 83.72196858631456 58.89932400664845 2 9.703320278917683 13.652784680021899 3 2.9468669854230325 6.2194598255289435 4 1.280746245511724 3.60407617539105 5 0.6688533048427218 2.3527282133980245 6 0.40803998339755587 1.7223636468860084 7 0.27585621771705754 1.3584751427422839 8 0.17621333184853255 0.9917441075110447 9 0.13592032915052454 0.860592993310492 >10 0.6426582112493617 7.800208852748019 >50 0.030225157501402172 1.4306730536806427 >100 0.009331368126005149 1.107569302133164 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.117057230575743E-5 0.0 5 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 6 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 7 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 8 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 9 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 10 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 11 0.0 0.0 0.0 6.117057230575743E-5 6.117057230575743E-5 12 0.0 0.0 0.0 6.117057230575743E-5 2.446822892230297E-4 13 0.0 0.0 0.0 6.117057230575743E-5 3.6702343383454464E-4 14 0.0 0.0 0.0 6.117057230575743E-5 3.6702343383454464E-4 15 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 16 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 17 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 18 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 19 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 20 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 21 0.0 6.117057230575743E-5 0.0 6.117057230575743E-5 4.2819400614030204E-4 22 0.0 6.117057230575743E-5 0.0 1.2234114461151486E-4 4.893645784460594E-4 23 0.0 6.117057230575743E-5 0.0 3.058528615287872E-4 4.893645784460594E-4 24 0.0 6.117057230575743E-5 0.0 4.893645784460594E-4 4.893645784460594E-4 25 0.0 6.117057230575743E-5 0.0 6.728762953633318E-4 4.893645784460594E-4 26 0.0 6.117057230575743E-5 0.0 8.563880122806041E-4 6.728762953633318E-4 27 0.0 6.117057230575743E-5 0.0 0.001101070301503634 6.728762953633318E-4 28 0.0 6.117057230575743E-5 0.0 0.0019574583137842378 7.340468676690893E-4 29 0.0 1.2234114461151486E-4 0.0 0.0028750168983705995 7.340468676690893E-4 30 0.0 1.8351171691727232E-4 0.0 0.004098428344485748 7.340468676690893E-4 31 0.0 1.8351171691727232E-4 0.0 0.010643679581201793 7.340468676690893E-4 32 0.0 1.8351171691727232E-4 0.0 0.020125118288594195 7.340468676690893E-4 33 0.0 1.8351171691727232E-4 0.0 0.02862782783909448 7.340468676690893E-4 34 0.0 1.8351171691727232E-4 0.0 0.03890448398646173 0.0010398997291978765 35 0.0 1.8351171691727232E-4 0.0 0.04918114013382898 0.0010398997291978765 36 0.0 1.8351171691727232E-4 0.0 0.06447378321026834 0.0010398997291978765 37 0.0 1.8351171691727232E-4 0.0 0.08576114237267192 0.0010398997291978765 38 0.0 1.8351171691727232E-4 0.0 0.11506184650712974 0.0010398997291978765 39 0.0 1.8351171691727232E-4 0.0 0.15035726672755179 0.0010398997291978765 40 0.0 1.8351171691727232E-4 0.0 0.18369522863418958 0.0010398997291978765 41 0.0 1.8351171691727232E-4 0.0 0.21746138454696767 0.0010398997291978765 42 0.0 1.8351171691727232E-4 0.0 0.2515945638935803 0.0010398997291978765 43 0.0 1.8351171691727232E-4 0.0 0.28994851272929023 0.0010398997291978765 44 0.0 1.8351171691727232E-4 0.0 0.3276295852696368 0.0010398997291978765 45 0.0 1.8351171691727232E-4 0.0 0.3644542697977028 0.0010398997291978765 46 0.0 1.8351171691727232E-4 0.0 0.4047045063748912 0.0010398997291978765 47 0.0 1.8351171691727232E-4 0.0 0.44544410753052566 0.001101070301503634 48 0.0 1.8351171691727232E-4 0.0 0.4894257490183652 0.0011622408738093913 49 0.0 1.8351171691727232E-4 0.0 0.5340190962292624 0.0012234114461151487 50 0.0 1.8351171691727232E-4 0.0 0.5836896009415374 0.0012234114461151487 51 0.0 1.8351171691727232E-4 0.0 0.6321978647800031 0.0012234114461151487 52 0.0 1.8351171691727232E-4 0.0 0.6834588043722278 0.001529264307643936 53 0.0 1.8351171691727232E-4 0.0 0.7331293090845029 0.001529264307643936 54 0.0 1.8351171691727232E-4 0.0 0.7865923892797348 0.001529264307643936 55 0.0 1.8351171691727232E-4 0.0 0.839749616613438 0.001529264307643936 56 0.0 1.8351171691727232E-4 0.0 0.8940690848209507 0.001529264307643936 57 0.0 1.8351171691727232E-4 0.0 0.9472874827269596 0.001529264307643936 58 0.0 1.8351171691727232E-4 0.0 1.0073569847312134 0.001529264307643936 59 0.0 1.8351171691727232E-4 0.0 1.0731765205322084 0.001529264307643936 60 0.0 1.8351171691727232E-4 0.0 1.138629032899369 0.001529264307643936 61 0.0 1.8351171691727232E-4 0.0 1.2061001741526194 0.0015904348799496934 62 0.0 1.8351171691727232E-4 0.0 1.2755287737196541 0.0019574583137842378 63 0.0 1.8351171691727232E-4 0.0 1.3460584435881924 0.0019574583137842378 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1740 0.0 42.42522 1 TCAACGC 2435 0.0 30.031927 4 TATCAAC 2450 0.0 29.566475 2 CAACGCA 2485 0.0 29.566475 5 ATCAACG 2495 0.0 29.309717 3 GGTATCA 845 0.0 28.984194 1 AACGCAG 2535 0.0 27.894733 6 TATACCG 80 9.575152E-5 25.870665 5 CGCGTAA 70 0.0013353906 24.63873 10 GCGTAAC 75 0.0019953235 22.996147 11 GTACATG 4880 0.0 22.831913 1 ACGCAGA 3110 0.0 21.960949 7 TACATGG 4970 0.0 21.723724 2 GTATTAC 160 5.7303623E-8 21.559547 1 ACATGGG 5090 0.0 20.87273 3 GTATAGA 265 0.0 20.827335 1 TATCGTT 85 0.0041249157 20.290716 37 CGCAGAG 3365 0.0 19.886713 8 AGAGTAC 3280 0.0 19.455582 11 GTATAAA 320 0.0 19.403593 1 >>END_MODULE