FastQCFastQC Report
Mon 6 Feb 2017
SRR3203983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634773
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32100.045.1314281
GGTATCA11150.042.594961
TCAACGC43000.032.8703464
ATCAACG43750.032.708053
TATCAAC43800.032.6707152
CAACGCA43400.032.002045
AACGCAG46400.030.2271256
ACGCAGA52300.026.4167987
CGCAGAG55350.024.6529678
AGAGTAC53700.023.58118611
GAGTACT35750.022.60962912
CAGAGTA58450.022.17546810
CTCGTAT11200.021.65619539
AGTACTT37300.021.48710313
GCAGAGT61850.021.3963229
GTACATG44100.020.99661
TATGCCG12100.020.7850143
TCGTATG12250.020.6485240
GTACTTT38850.020.3659714
TACTTTT39050.020.08623915
Produced by FastQC (version 0.11.5)