FastQCFastQC Report
Mon 6 Feb 2017
SRR3203982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734592
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38300.036.656621
GGTATCA18100.028.7776411
TCAACGC45750.028.575084
ATCAACG46000.028.1948243
CAACGCA46550.027.9357955
TATCAAC49250.027.4548572
AACGCAG48250.026.737066
ACGCAGA54800.023.0377437
GTACATG81150.021.721471
CGCAGAG57900.021.6851378
TACATGG81500.020.8654942
AGAGTAC60250.019.92331311
GCGATAC5000.019.31645414
ACATGGG87550.018.675043
CAGAGTA67300.018.45129410
CGATACT5650.017.09420815
TGCGATA5450.017.08860613
GCAGAGT74400.016.6904839
GTGCGAT5650.016.483712
CATGGGG67000.016.4745884
Produced by FastQC (version 0.11.5)