FastQCFastQC Report
Mon 6 Feb 2017
SRR3203982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734592
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61650.041.8329161
GGTATCA23700.035.647451
TATCAAC80800.030.9674742
TCAACGC80400.030.638664
ATCAACG81650.030.3113943
CAACGCA80800.030.2629875
AACGCAG84300.028.9660036
ACGCAGA96750.024.8369987
CGCAGAG98200.024.2617078
CTCGTAT15200.023.70801739
TATGCCG15050.023.12721343
AGAGTAC99750.022.96910911
GTACATG83000.022.6242471
TCTCGTA13800.022.59886438
GAGTACT71500.022.08804312
CAGAGTA106150.022.08620510
TACATGG81650.022.0209272
AATCTCG13150.021.8802736
CCGTCTT16350.021.40265847
TCGTATG16650.021.22713740
Produced by FastQC (version 0.11.5)