FastQCFastQC Report
Mon 6 Feb 2017
SRR3203981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650261
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36400.039.328991
TCAACGC43500.031.8775544
ATCAACG44550.031.2036613
CAACGCA44650.030.8247575
TATCAAC48400.029.2204382
AACGCAG47200.028.9401916
GGTATCA15650.026.8912831
ACGCAGA54950.024.1052557
GTACATG78550.022.0456871
CGCAGAG57750.022.040568
TACATGG80700.020.6880742
AGAGTAC57750.020.48756611
ACATGGG83250.019.0599523
CAGAGTA66500.018.1030610
GAGTACT27800.017.74350712
GCAGAGT69650.017.3338559
CATGGGG67050.017.285774
GTATATA14400.015.810571
ATGGGGG38850.015.0052745
CGGACCG1851.0237038E-414.9164855
Produced by FastQC (version 0.11.5)