FastQCFastQC Report
Mon 6 Feb 2017
SRR3203981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650261
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57650.042.2353021
GGTATCA23200.038.2710841
ATCAACG78900.029.844173
TCAACGC78650.029.8358844
TATCAAC81000.029.7015862
CAACGCA80150.029.1827965
AACGCAG83550.027.9136096
ACGCAGA96600.024.1340717
CGCAGAG101000.022.8464588
GTACATG77650.022.297281
AGAGTAC100750.022.03412611
GAGTACT73500.020.775112
CAGAGTA109300.020.57973110
TACATGG81600.020.450042
GCAGAGT114450.020.0402589
ACATGGG81750.019.703423
AATCTCG12150.019.69611736
AGTACTT78050.019.47733713
CTCGTAT13700.018.72958639
GTACTTT82050.018.61202814
Produced by FastQC (version 0.11.5)