FastQCFastQC Report
Mon 6 Feb 2017
SRR3203980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665227
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37500.035.507741
TCAACGC44050.029.0524754
ATCAACG44900.028.348833
CAACGCA46700.027.7732125
TATCAAC47750.026.7290462
AACGCAG48600.026.4035196
GGTATCA18750.025.5729311
ACGCAGA55750.022.336627
GTACATG83200.021.1452981
CGCAGAG58150.020.8808448
TACATGG82600.019.961952
AGAGTAC57800.019.45561211
TCAATCG3550.018.4620745
ACATGGG84900.018.1616343
GCGATAC4200.018.06876614
CGAATCG3750.017.47766337
CAGAGTA65850.017.39151610
GAGTACT25650.017.3483212
GCAGAGT72450.016.4737649
CGCCCAA6300.016.42637650
Produced by FastQC (version 0.11.5)