FastQCFastQC Report
Mon 6 Feb 2017
SRR3203980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665227
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61150.041.8930741
GGTATCA23900.034.87141
TATCAAC85050.029.461972
TCAACGC84650.029.3455834
ATCAACG85250.029.3127633
CAACGCA84950.028.9103455
AACGCAG87700.027.8882686
ACGCAGA101300.024.2005837
CTCGTAT19750.023.69261239
CGCAGAG104900.023.305058
TCGTATG19950.023.10696640
TATGCCG19550.023.08045843
TCTCGTA18200.023.04412738
TCAATCG3800.021.8865645
AGAGTAC107550.021.66238811
GCCGTCT20700.021.42838746
CCGTCTT21400.020.88939347
ATCTCGT20300.020.81355537
GTACATG83050.020.522881
ATGCCGT21650.020.36329344
Produced by FastQC (version 0.11.5)