FastQCFastQC Report
Mon 6 Feb 2017
SRR3203979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4413684
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42800.034.33311
GGTATCA20250.027.765771
TCAACGC52050.027.1706734
CAACGCA51900.026.9833585
ATCAACG52150.026.9201453
AACGCAG54600.025.6490156
TATCAAC57000.025.1136912
GTACATG98200.021.9541021
ACGCAGA64200.021.222647
TACATGG97450.020.6005332
CGCAGAG68050.019.9712568
ACATGGG98950.019.242463
AGAGTAC70200.018.27860811
TCAATCG4150.017.4547445
CAGAGTA80350.016.52768910
CATGGGG74750.015.9200784
GCAGAGT83600.015.7201259
GTATTAG12450.015.5155011
CGCACGA4300.015.24131535
TCGCAAT5000.015.17765649
Produced by FastQC (version 0.11.5)