FastQCFastQC Report
Mon 6 Feb 2017
SRR3203979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4413684
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68350.041.4315951
GGTATCA27500.039.5011751
TATCAAC96500.028.7252242
TCAACGC96100.028.4449984
ATCAACG97150.028.3238053
CAACGCA97950.027.6168065
AACGCAG99950.026.996556
ACGCAGA115200.023.3820427
CGCAGAG119800.022.5411578
GTACATG94200.022.1204811
GAGTACT89250.021.25415812
TACATGG94050.020.9436192
AGAGTAC123300.020.93984611
AGTACTT97450.019.95853213
CAGAGTA133600.019.85190810
TATGCCG13050.019.80135243
GTACTTT99150.019.13304514
GCAGAGT138750.019.1157119
ACATGGG101150.018.7996033
TCGTATG14900.018.14433340
Produced by FastQC (version 0.11.5)