FastQCFastQC Report
Mon 6 Feb 2017
SRR3203978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916134
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36200.036.5904241
TCAACGC43450.029.214054
ATCAACG44000.028.927273
CAACGCA44500.028.4472185
TATCAAC50450.025.9126472
AACGCAG48300.025.7806476
GGTATCA20150.022.4254461
ACGCAGA54000.022.2928417
GTACATG88450.021.8781131
TACATGG90000.020.7725852
CGCAGAG57800.020.5288378
ACATGGG89200.020.1854953
AGAGTAC59250.019.56071311
CATGGGG62700.017.2191054
CCTATAC5850.017.0992033
ATACCGT3054.7293724E-1116.963886
CAGAGTA70250.016.8906310
GCAGAGT71700.016.4047269
GAGTACT26950.015.9987112
GTATATA17750.015.7409711
Produced by FastQC (version 0.11.5)