FastQCFastQC Report
Mon 6 Feb 2017
SRR3203978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916134
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63350.042.2090871
GGTATCA24700.033.1821941
ATCAACG84750.030.9247883
TCAACGC84450.030.7388044
TATCAAC85800.030.5841562
CAACGCA85100.030.3713465
AACGCAG89650.028.7538436
CTCGTAT16350.025.02079439
ACGCAGA102750.024.943057
TATGCCG16550.024.5687343
CGCAGAG106800.023.7418888
AATCTCG14700.022.8908636
TCGTATG18400.022.60939840
AGAGTAC109450.022.52378311
TCTCGTA15250.022.28031238
GTACATG91050.021.9415191
CCGTCTT18050.021.89200247
GAGTACT77650.021.7374812
GACGTAG19050.021.4773326
CAGAGTA118900.021.1783910
Produced by FastQC (version 0.11.5)