##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3203978_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3916134 Sequences flagged as poor quality 0 Sequence length 75 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.665857194876374 35.0 32.0 35.0 27.0 35.0 2 32.784084252479616 35.0 32.0 35.0 27.0 35.0 3 32.74657966249367 35.0 32.0 35.0 30.0 35.0 4 32.88614383471046 35.0 33.0 35.0 30.0 35.0 5 32.922179629195526 35.0 33.0 35.0 30.0 35.0 6 36.46461816679409 39.0 36.0 40.0 27.0 40.0 7 36.28433169038649 39.0 36.0 40.0 27.0 40.0 8 36.13967908146146 39.0 36.0 40.0 27.0 40.0 9 36.10619325079275 39.0 36.0 40.0 27.0 40.0 10 36.06367580884617 39.0 36.0 40.0 27.0 40.0 11 36.1397487930699 39.0 36.0 40.0 27.0 40.0 12 36.14268587336388 39.0 36.0 40.0 27.0 40.0 13 36.11852454487002 39.0 36.0 40.0 27.0 40.0 14 36.05942084719266 39.0 36.0 40.0 27.0 40.0 15 36.022951717178216 39.0 36.0 40.0 27.0 40.0 16 36.009204996560385 39.0 36.0 40.0 27.0 40.0 17 35.724993067142236 39.0 36.0 40.0 27.0 40.0 18 35.67280384174801 39.0 35.0 40.0 27.0 40.0 19 35.89104611844232 39.0 36.0 40.0 27.0 40.0 20 35.87706038659555 39.0 36.0 40.0 27.0 40.0 21 35.643548458760606 39.0 36.0 40.0 27.0 40.0 22 35.63783440505355 39.0 35.0 40.0 27.0 40.0 23 35.42445508759404 39.0 35.0 40.0 27.0 40.0 24 35.468982930614736 39.0 35.0 40.0 27.0 40.0 25 35.22514985442275 39.0 35.0 40.0 16.0 40.0 26 35.27021138704651 39.0 35.0 40.0 16.0 40.0 27 35.36825399743727 39.0 35.0 40.0 27.0 40.0 28 35.347915827190796 39.0 35.0 40.0 27.0 40.0 29 35.32098084488427 39.0 35.0 40.0 27.0 40.0 30 35.2539739447118 39.0 35.0 40.0 16.0 40.0 31 35.29009579345344 39.0 35.0 40.0 16.0 40.0 32 35.05750416099143 39.0 35.0 40.0 16.0 40.0 33 35.278047686825836 39.0 35.0 40.0 27.0 40.0 34 35.29927780816489 39.0 35.0 40.0 16.0 40.0 35 35.34307712657432 39.0 35.0 40.0 27.0 40.0 36 35.33602833815186 39.0 35.0 40.0 27.0 40.0 37 35.122652340292746 39.0 35.0 40.0 16.0 40.0 38 35.17451241453944 39.0 35.0 40.0 16.0 40.0 39 35.20457343900898 39.0 35.0 40.0 16.0 40.0 40 35.26907429623195 39.0 35.0 40.0 27.0 40.0 41 35.40108561147295 39.0 35.0 40.0 27.0 40.0 42 35.115093610177794 39.0 35.0 40.0 16.0 40.0 43 35.06913042301412 39.0 35.0 40.0 16.0 40.0 44 35.12645302739896 39.0 35.0 40.0 16.0 40.0 45 34.93966983765111 39.0 34.0 40.0 16.0 40.0 46 34.86878819774809 39.0 34.0 40.0 16.0 40.0 47 34.9636639093555 39.0 34.0 40.0 16.0 40.0 48 34.84674094400243 39.0 34.0 40.0 16.0 40.0 49 34.87235191645638 39.0 34.0 40.0 16.0 40.0 50 34.791804366244875 39.0 34.0 40.0 16.0 40.0 51 34.46733462133829 39.0 34.0 40.0 16.0 40.0 52 34.77950116109408 39.0 34.0 40.0 16.0 40.0 53 34.7883884974314 39.0 34.0 40.0 16.0 40.0 54 34.72329394244426 39.0 34.0 40.0 16.0 40.0 55 34.64077582636345 39.0 34.0 40.0 16.0 40.0 56 34.666380414970476 39.0 34.0 40.0 16.0 40.0 57 34.61089406031561 39.0 34.0 40.0 16.0 40.0 58 34.6582029624114 39.0 34.0 40.0 16.0 40.0 59 34.61913637275946 39.0 34.0 40.0 16.0 40.0 60 34.302385464848754 39.0 34.0 40.0 16.0 40.0 61 34.5578350996161 39.0 34.0 40.0 16.0 40.0 62 34.278300487164124 39.0 34.0 40.0 16.0 40.0 63 34.448796440571236 39.0 34.0 40.0 16.0 40.0 64 34.4377061663365 39.0 34.0 40.0 16.0 40.0 65 34.38292050272028 39.0 34.0 40.0 16.0 40.0 66 34.39593793266523 39.0 34.0 40.0 16.0 40.0 67 34.37972091864068 39.0 34.0 40.0 16.0 40.0 68 34.297476031208326 39.0 34.0 40.0 16.0 40.0 69 34.18431953554194 39.0 34.0 40.0 16.0 40.0 70 34.10413611995912 39.0 34.0 40.0 16.0 40.0 71 34.06391073441307 39.0 34.0 40.0 16.0 40.0 72 34.07692152515721 39.0 34.0 40.0 16.0 40.0 73 33.91461553664915 39.0 34.0 40.0 16.0 40.0 74 33.841427029820736 39.0 34.0 40.0 16.0 40.0 75 33.782673677662714 39.0 34.0 40.0 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1102.0 3 2142.0 4 1903.0 5 1837.0 6 2350.0 7 3342.0 8 5218.0 9 8631.0 10 8008.0 11 5728.0 12 5865.0 13 6607.0 14 3822.0 15 4390.0 16 5237.0 17 6040.0 18 7323.0 19 10338.0 20 18985.0 21 31199.0 22 42573.0 23 53201.0 24 63584.0 25 72137.0 26 77579.0 27 84569.0 28 91825.0 29 100003.0 30 108418.0 31 117551.0 32 127829.0 33 140634.0 34 157540.0 35 181933.0 36 220633.0 37 300724.0 38 516809.0 39 1318525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15872977740385 18.337232475964942 12.960990343041065 25.54304740359014 2 14.987944743463835 20.001205270299742 39.36637510360983 25.64447488262659 3 18.100383602965824 25.182681460348082 29.554518830675157 27.16241610601094 4 12.011632921689579 16.785341271885184 38.128630497610665 33.07439530881457 5 13.459250518320548 37.93863809951601 34.49306731106358 14.109044071099866 6 31.382622000680094 38.02020806917619 17.4071752978083 13.189994632335415 7 27.672776263529286 32.14494192486774 22.073223234955698 18.109058576647275 8 26.05244083524075 35.2775511809455 20.08754101090508 18.58246697290867 9 26.431279653531075 15.250256074736281 18.985486927611355 39.33297734412129 10 13.409134377340482 29.377505184328705 34.8108553114568 22.40250512687401 11 35.15449862478643 22.842079109444835 23.930324895642855 18.073097370125883 12 23.328159784518885 24.524357813463176 31.026277848682128 21.121204553335815 13 28.063873040419747 21.781699612444175 26.193048611609417 23.961378735526665 14 21.856803265941497 20.556871932019305 27.781314502127525 29.80501029991167 15 23.226953614581515 28.879956240749422 24.165341325218872 23.72774881945019 16 23.34477653828877 28.011693979944642 25.466685359873605 23.17684412189299 17 22.00147591971569 27.565815900479674 26.990396300797343 23.442311879007292 18 22.19846900743957 27.17148957786033 27.89972775687512 22.730313657824983 19 23.099235674190734 26.7663700730408 28.069728703414714 22.064665549353748 20 23.170153323333633 26.35364355217317 28.068030988001127 22.408172136492066 21 23.37121432613896 26.1593996823833 27.93601200512146 22.533373986356278 22 22.78201300040107 26.41077020760227 27.896215276958596 22.91100151503806 23 22.880910307594007 26.13696763519513 27.762852660771575 23.219269396439287 24 22.65108284527783 26.19890851171539 28.02049936000593 23.12950928300085 25 22.840404250344235 26.31241069340885 27.9620170949053 22.88516796134161 26 22.82958481708873 26.486002336817897 27.923734197811324 22.760678648282045 27 22.802878909314252 26.69785717252226 27.73675536364661 22.762508554516874 28 22.737506286254153 26.64743455435619 27.658005206467383 22.957053952922273 29 23.19602442018065 26.367598735549336 27.634345303170466 22.80203154109955 30 22.81200049943607 26.407475856290368 27.904055647909953 22.876467996363612 31 22.881988584330266 26.346593314778232 27.72265928634241 23.048758814549096 32 22.84379605328066 26.454760666282013 27.67636550156333 23.025077778874003 33 22.749610134699424 26.542928858253457 27.774440764881035 22.933020242166084 34 23.146890825436564 26.48808951374052 27.705488489320988 22.659531171501925 35 23.12916674573408 26.338269362241405 27.75853376708433 22.774030124940186 36 23.02271950031295 26.57140091755551 27.525968369499992 22.87991121263155 37 23.076197965695485 26.276876669804512 27.75605390894053 22.890871455559473 38 22.904934550365073 26.379846490973506 27.670008473822083 23.04521048483934 39 23.13095948857967 26.607282868837046 27.377449947746097 22.88430769483719 40 23.128528395604373 26.202356502668977 27.951290277814323 22.717824823912327 41 22.885263035354296 26.40276889576999 27.54046648447368 23.171501584402037 42 23.24726873708012 26.260724756485924 27.548159837886 22.943846668547955 43 22.98002485094449 26.036341226976162 27.824698731809644 23.158935190269705 44 22.98338418314713 26.612421126838758 27.383160555866148 23.021034134147964 45 22.967974012055574 26.452348170767664 27.372105008114822 23.207572809061936 46 23.33618868608563 26.364819729470497 27.02246532746264 23.276526256981235 47 23.02620176011667 26.440619907307223 27.2272983203921 23.305880012184 48 22.920980437402253 26.658128861882417 27.08460887624029 23.33628182447504 49 23.220075530170572 26.29056669588828 27.175054666039845 23.314303107901303 50 23.331856503181143 26.458914542606127 27.199406544102533 23.0098224101102 51 22.972211191324064 26.75673053664493 26.710086006511606 23.560972265519396 52 23.07443807117167 26.64976376273102 27.028527944915233 23.247270221182077 53 23.06315503938377 26.621103094142097 27.16259462641443 23.153147240059706 54 22.879672432934132 26.642693967340726 27.15940178500463 23.31823181472052 55 22.946754606230694 26.55159171039027 27.145042482002147 23.356611201376893 56 23.044741954660505 26.879952687091297 26.97647837518156 23.098826983066637 57 23.103885474828235 26.66808818703803 26.75432425220155 23.47370208593218 58 22.923446212667415 26.63998777502351 27.209264522031113 23.227301490277966 59 23.0262821887864 26.83347123055394 27.062150788057078 23.07809579260258 60 23.069228631553717 26.89828106130182 26.960575672456383 23.071914634688078 61 23.14027638321844 26.742129492963763 27.177981068495193 22.939613055322607 62 22.93069528700005 27.012139222307873 26.983190443152637 23.073975047539445 63 22.827359041518843 26.831542871618165 27.208863877157192 23.1322342097058 64 22.97356855865818 26.77197776488424 27.038084340324133 23.216369336133447 65 22.93962031614549 26.712493266900943 27.06116312504158 23.286723291911983 66 22.911344109033223 27.017966536967364 26.917091194374272 23.15359815962514 67 22.894540190695682 26.82578064224818 26.970919598177325 23.308759568878816 68 22.972138847864986 26.86542693473697 27.09728610614752 23.06514811125052 69 23.175339700669557 26.790582537416306 26.80627523139031 23.227802530523828 70 22.901187929520102 26.51663900285955 27.064708646290587 23.51746442132976 71 23.17264296241051 26.478382913728325 26.951974233689807 23.396999890171358 72 22.92114352778197 26.71097024949155 26.996872748723355 23.371013474003117 73 23.080215761191592 26.578647610485667 26.97168134360378 23.36945528471896 74 23.00765415173687 26.685227814788565 26.815587546247006 23.491530487227557 75 23.033620011641567 26.702707150161416 26.956976789942257 23.30669604825476 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 125.0 1 141.5 2 308.5 3 519.5 4 580.0 5 461.0 6 445.5 7 741.0 8 933.0 9 1643.5 10 3052.5 11 3726.0 12 3701.0 13 3658.0 14 3843.5 15 4471.5 16 4871.0 17 5354.0 18 6408.5 19 7816.5 20 8653.0 21 9911.0 22 13611.0 23 18874.5 24 21696.0 25 24920.0 26 32848.5 27 41361.5 28 45170.0 29 51000.0 30 62312.0 31 73935.5 32 80077.0 33 86609.5 34 99359.5 35 111371.5 36 117166.0 37 122581.0 38 132823.5 39 143648.5 40 149646.0 41 156572.0 42 166502.0 43 173553.5 44 177601.0 45 181640.0 46 187121.0 47 190776.0 48 192989.0 49 194813.5 50 194705.5 51 189737.0 52 186701.0 53 183075.5 54 173985.5 55 160407.5 56 152294.0 57 146045.5 58 129367.0 59 106896.5 60 94856.0 61 86327.0 62 67919.0 63 50308.5 64 42577.0 65 35496.0 66 23695.0 67 16289.5 68 13604.0 69 11539.5 70 7673.0 71 4720.0 72 3569.0 73 3185.5 74 2380.5 75 1566.0 76 1173.0 77 942.5 78 508.0 79 251.0 80 198.0 81 166.5 82 92.5 83 34.5 84 19.0 85 13.0 86 4.0 87 2.0 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08447106253258954 2 0.0 3 0.04264409746959629 4 0.052858252552134326 5 0.003089781912467755 6 0.049921682965904636 7 0.0 8 0.0181811960469177 9 0.007277585496308349 10 1.2767693853172544E-4 11 3.830308155951762E-4 12 0.0015321232623807049 13 0.0013533755484362894 14 0.0020683664042139517 15 0.004136732808427903 16 0.006971160843832208 17 0.06746449432016371 18 0.41650770887819466 19 0.020121885512599923 20 0.017440669803433693 21 0.42044015858497186 22 0.4229170911924873 23 1.1289705612729288 24 0.7302610176260567 25 1.7117391795071362 26 1.1287152073958653 27 1.0093372698687022 28 0.4277943502443992 29 1.0129377595352969 30 1.0153380859796932 31 0.4278964917952246 32 1.1301962598828335 33 0.6068229534535846 34 0.4278454210198119 35 0.4215381802563446 36 1.0650554858439472 37 1.1248082930767946 38 1.0083669251358611 39 0.6026862206451566 40 1.0053792847742187 41 0.41469469635104417 42 1.0049707185709171 43 0.7188977700967332 44 1.1138280763630664 45 0.4050933905734584 46 0.404914642859514 47 0.406983009263728 48 0.404327328942268 49 0.0021194371796266417 50 0.0022726495058647124 51 1.2953846829551798 52 0.4039187627389665 53 0.4033569842094269 54 0.4050933905734584 55 0.5952043520471976 56 0.0019917602410949166 57 0.0035494188911819667 58 0.40657444306042645 59 0.004443157460904045 60 1.1289194904975162 61 0.0031153173001741002 62 0.5886162220189606 63 0.002681215709166234 64 0.40389322735126015 65 0.5886672927943732 66 0.010469508959601486 67 0.01470838331885477 68 0.010086478144006308 69 0.41443934247398073 70 0.6016903405246092 71 0.02410540599478976 72 0.6102446954062348 73 0.01772155906820349 74 1.0198067788283036 75 0.06567701718071955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3916134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.170065781753166 #Duplication Level Percentage of deduplicated Percentage of total 1 74.47308395996566 42.576311089612645 2 13.248436659662618 15.148279906766044 3 5.102667532702388 8.751595155210351 4 2.520664160226849 5.764261434155063 5 1.36778470236334 3.9098170704693924 6 0.833247442914051 2.8582086674324345 7 0.5658277618015153 2.2643887254334354 8 0.378102423240727 1.7292912327130112 9 0.2616143241349992 1.3460857308222118 >10 1.1763976802738347 11.46710098196227 >50 0.05314808605992461 2.070441753697407 >100 0.018300145313110673 1.8151187430537725 >500 6.344811843963208E-4 0.2323306793897824 >1k 9.064015647871994E-5 0.06676882928217581 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5535387706345085E-5 0.0 4 0.0 0.0 0.0 2.5535387706345085E-5 0.0 5 0.0 0.0 0.0 2.5535387706345085E-5 0.0 6 0.0 0.0 0.0 2.5535387706345085E-5 0.0 7 0.0 0.0 0.0 2.5535387706345085E-5 2.5535387706345085E-5 8 0.0 0.0 0.0 2.5535387706345085E-5 2.5535387706345085E-5 9 0.0 0.0 0.0 5.107077541269017E-5 2.5535387706345085E-5 10 0.0 0.0 0.0 5.107077541269017E-5 2.5535387706345085E-5 11 0.0 0.0 0.0 5.107077541269017E-5 2.5535387706345085E-5 12 0.0 0.0 0.0 7.660616311903525E-5 5.107077541269017E-5 13 0.0 0.0 0.0 1.0214155082538034E-4 5.107077541269017E-5 14 0.0 0.0 0.0 1.0214155082538034E-4 5.107077541269017E-5 15 0.0 0.0 0.0 1.0214155082538034E-4 1.0214155082538034E-4 16 0.0 0.0 0.0 1.0214155082538034E-4 1.0214155082538034E-4 17 0.0 0.0 0.0 1.0214155082538034E-4 1.0214155082538034E-4 18 0.0 0.0 0.0 1.0214155082538034E-4 1.0214155082538034E-4 19 0.0 0.0 0.0 1.0214155082538034E-4 1.0214155082538034E-4 20 0.0 0.0 0.0 1.0214155082538034E-4 1.0214155082538034E-4 21 0.0 0.0 0.0 1.2767693853172542E-4 1.0214155082538034E-4 22 0.0 0.0 0.0 1.2767693853172542E-4 1.0214155082538034E-4 23 0.0 0.0 0.0 1.2767693853172542E-4 1.0214155082538034E-4 24 0.0 0.0 0.0 1.2767693853172542E-4 1.0214155082538034E-4 25 0.0 0.0 0.0 1.532123262380705E-4 1.0214155082538034E-4 26 0.0 0.0 0.0 1.787477139444156E-4 1.0214155082538034E-4 27 0.0 0.0 0.0 2.2981848935710577E-4 1.0214155082538034E-4 28 0.0 0.0 0.0 5.617785295395918E-4 1.0214155082538034E-4 29 0.0 0.0 0.0 0.0013023047730235993 1.0214155082538034E-4 30 0.0 0.0 0.0 0.002400326444396438 1.0214155082538034E-4 31 0.0 0.0 0.0 0.005822068397046679 1.0214155082538034E-4 32 0.0 0.0 0.0 0.009728982716117477 1.0214155082538034E-4 33 0.0 0.0 0.0 0.012997512342529648 1.0214155082538034E-4 34 0.0 0.0 0.0 0.01631711274435451 1.0214155082538034E-4 35 0.0 0.0 0.0 0.019994208574068202 1.0214155082538034E-4 36 0.0 0.0 0.0 0.024488436810384935 1.0214155082538034E-4 37 0.0 0.0 0.0 0.032685296264121706 1.0214155082538034E-4 38 0.0 0.0 0.0 0.043103734448310504 1.0214155082538034E-4 39 0.0 0.0 0.0 0.0545435881407531 1.0214155082538034E-4 40 0.0 0.0 0.0 0.06641754342420357 1.0214155082538034E-4 41 2.5535387706345085E-5 0.0 0.0 0.08000236968397915 1.0214155082538034E-4 42 2.5535387706345085E-5 0.0 0.0 0.09292327586338976 1.0214155082538034E-4 43 2.5535387706345085E-5 0.0 0.0 0.10658470828628439 1.0214155082538034E-4 44 5.107077541269017E-5 0.0 0.0 0.12075684846330591 1.0214155082538034E-4 45 5.107077541269017E-5 0.0 0.0 0.1364355765150018 1.0214155082538034E-4 46 5.107077541269017E-5 0.0 0.0 0.15323786162577685 1.0214155082538034E-4 47 5.107077541269017E-5 0.0 0.0 0.17141905767269455 1.0214155082538034E-4 48 5.107077541269017E-5 0.0 0.0 0.189804536821263 1.0214155082538034E-4 49 5.107077541269017E-5 0.0 0.0 0.209339108416617 1.0214155082538034E-4 50 5.107077541269017E-5 0.0 0.0 0.2295631354800423 1.0214155082538034E-4 51 5.107077541269017E-5 0.0 0.0 0.25019572874676915 1.0214155082538034E-4 52 5.107077541269017E-5 0.0 0.0 0.2734073961718368 1.0214155082538034E-4 53 5.107077541269017E-5 0.0 0.0 0.29646585127066644 1.2767693853172542E-4 54 5.107077541269017E-5 0.0 0.0 0.31947323559408336 1.2767693853172542E-4 55 5.107077541269017E-5 0.0 0.0 0.3447788048110713 1.2767693853172542E-4 56 5.107077541269017E-5 0.0 0.0 0.36936938317228163 1.2767693853172542E-4 57 5.107077541269017E-5 0.0 0.0 0.3971518849967851 1.2767693853172542E-4 58 5.107077541269017E-5 0.0 0.0 0.4249088514335822 1.2767693853172542E-4 59 5.107077541269017E-5 0.0 0.0 0.4567770152911009 1.2767693853172542E-4 60 5.107077541269017E-5 0.0 0.0 0.4898198069831114 1.532123262380705E-4 61 5.107077541269017E-5 0.0 0.0 0.5243691865497963 1.532123262380705E-4 62 5.107077541269017E-5 0.0 0.0 0.5589951722796003 1.532123262380705E-4 63 5.107077541269017E-5 0.0 0.0 0.5964300506571021 1.532123262380705E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6335 0.0 42.209087 1 GGTATCA 2470 0.0 33.182194 1 ATCAACG 8475 0.0 30.924788 3 TCAACGC 8445 0.0 30.738804 4 TATCAAC 8580 0.0 30.584156 2 CAACGCA 8510 0.0 30.371346 5 AACGCAG 8965 0.0 28.753843 6 CTCGTAT 1635 0.0 25.020794 39 ACGCAGA 10275 0.0 24.94305 7 TATGCCG 1655 0.0 24.56873 43 CGCAGAG 10680 0.0 23.741888 8 AATCTCG 1470 0.0 22.89086 36 TCGTATG 1840 0.0 22.609398 40 AGAGTAC 10945 0.0 22.523783 11 TCTCGTA 1525 0.0 22.280312 38 GTACATG 9105 0.0 21.941519 1 CCGTCTT 1805 0.0 21.892002 47 GAGTACT 7765 0.0 21.73748 12 GACGTAG 1905 0.0 21.47733 26 CAGAGTA 11890 0.0 21.17839 10 >>END_MODULE