FastQCFastQC Report
Mon 6 Feb 2017
SRR3203977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339601
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31450.035.537911
TCAACGC36750.029.0972794
CAACGCA37500.028.975265
ATCAACG38250.028.1365723
AACGCAG39600.027.177376
TATCAAC42250.025.9626162
GGTATCA14450.025.7824081
ACGCAGA45750.023.4486227
CGCAGAG48650.021.767258
GTACATG72500.021.268511
TACATGG72600.020.430542
AGAGTAC50050.020.0556611
TGCGATA4050.019.58938613
ACATGGG75050.018.9362753
GCGATAC4250.018.66753414
GTGCGAT4500.018.3969912
CAGAGTA58750.017.7902810
GGTGCGA4800.017.24717711
GCAGAGT61450.016.9524759
CATGGGG54800.016.8065574
Produced by FastQC (version 0.11.5)