FastQCFastQC Report
Mon 6 Feb 2017
SRR3203977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339601
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46850.040.4141161
GGTATCA19850.032.9450651
TATCAAC63100.029.9341342
ATCAACG61650.029.8065153
TCAACGC61650.029.5196954
CAACGCA61850.029.3552915
AACGCAG64600.028.1056486
TATGCCG16150.026.22682843
CTCGTAT15350.024.57828339
AATCTCG14550.024.54254236
ACGCAGA75450.024.0099967
CGCAGAG77800.022.9772268
TCGTATG17650.022.9442940
TCTCGTA15100.022.92360338
ATCTCGT16100.022.71253637
GAGTACT52900.022.2953112
AGAGTAC80450.021.55058511
CGTATGC19800.021.15178541
GCCGTCT19650.020.25209446
TGCCGTC20700.019.72633245
Produced by FastQC (version 0.11.5)