FastQCFastQC Report
Mon 6 Feb 2017
SRR3203976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682104
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.036.311521
TCAACGC33750.030.049244
ATCAACG35000.028.9760553
CAACGCA35850.028.5776985
TATCAAC37300.027.8366932
AACGCAG36950.027.5402266
GGTATCA14700.025.8132921
CGTGCCG3750.023.91674210
ACGCAGA43750.023.1019977
CGCAGAG44800.021.6365628
TCAATCG2451.8189894E-1221.11957245
GCACCGT6150.020.753276
GTACATG66150.020.7027821
TACATGG65350.020.1640472
ACATGGG68850.018.788293
AGAGTAC47050.018.76896511
TCGCAAT3150.018.61685849
GTATAAT11800.018.4173261
CAATCGC3750.018.39749546
AATCGCA3200.018.32562847
Produced by FastQC (version 0.11.5)